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|Antigen||Oncostatin M (OSM) Antibodies|
|Epitope||AA 26-220 Alternatives|
|Conjugate||This Oncostatin M antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
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Product Details anti-Oncostatin M AntibodyTarget Details Oncostatin M Application Details Handling Images
|Purpose||Rabbit IgG polyclonal antibody for Oncostatin-M(OSM) detection. Tested with WB, IHC-P, ELISA in Human.|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Oncostatin-M(OSM) detection. Tested with WB, IHC-P, ELISA in Human.
Gene Name: oncostatin M
Protein Name: Oncostatin-M
|Purification||Immunogen affinity purified.|
|Immunogen||E. coli-derived human Oncostatin M recombinant protein (Position: A26-R220). Human Oncostatin M shares 47.1% and 47.6% amino acid (aa) sequence identity with mouse and rat Oncostatin M, respectively.|
Target Details Oncostatin MProduct Details anti-Oncostatin M Antibody Application Details Handling Images back to top
|Alternative Name||OSM (OSM Antibody Abstract)|
OSM (ONCOSTATIN M) is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. This gene encodes a growth regulator which inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. OSM is mapped on 22q12.2. It has the ability to inhibit the growth of human A375 melanoma cells but not normal human fibroblasts. Treatment with recombinant OSM leads to the inhibition of proliferation and changes in cellular morphology of a number of tumor cell lines derived from a wide variety of tissue types. OSM also has the ability to inhibit the proliferation of murine M1 myeloid leukemic cells and can induce their differentiation into macrophage-like cells, a function shared by LIF, CSF3, and IL6. The direction of gene transcription was telomeric to centromeric, with the OSM gene located upstream of the LIF gene.
Synonyms: MGC20461 antibody|ONCM_HUMAN antibody|Oncostatin M antibody|Oncostatin M precursor antibody|Oncostatin-M antibody|OSM antibody
|Pathways||JAK-STAT Signaling, Negative Regulation of Hormone Secretion|
Application DetailsProduct Details anti-Oncostatin M Antibody Target Details Oncostatin M Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
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|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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