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Human Oncostatin M ELISA Kit

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Antigen
Oncostatin M (OSM) ELISA Kits
  • OncoM
  • OSM
Epitope
AA 26-220
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
19
11
10
6
5
4
3
3
3
2
2
1
Methode Type
Sandwich ELISA
Detection Range
15.6-1000 pg/mL
Minimum Detection Limit
15.6 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN1672746
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32670403 ABIN414913 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see 1
0.32670403 ABIN1116292 Sandwich ELISA Serum, Plasma, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.32670403 ABIN2708349 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.001210097 ABIN3032974 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 1.4-1000 pg/mL 1.4 pg/mL Log in to see
0.001210097 ABIN365749 Sandwich ELISA Log in to see
0.001210097 ABIN511452 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 50-1000 pg/mL 50 pg/mL Log in to see
0.001210097 ABIN456039 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.001210097 ABIN625069 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine Log in to see 2
0.001210097 ABIN1029426 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.001210097 ABIN771036 Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids Log in to see
0.001210097 ABIN2042626 Sandwich ELISA 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.001210097 ABIN2116282 0.625-40 ng/mL 0.625 ng/mL Log in to see
0.001210097 ABIN2685137 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum, Urine Log in to see
0.001210097 ABIN2537371 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Culture Supernatant, Biological Fluids 15.62-1000 pg/mL 15.62 pg/mL Log in to see
0.001210097 ABIN2950058 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.001210097 ABIN2099605 Sandwich ELISA Log in to see

Top referenced Oncostatin M ELISA Kit for ELISA

Similar Oncostatin M ELISA Kits

Application / Reactivity Human
ELISA 17 ELISA Kits

General

Antigen Oncostatin M (OSM) ELISA Kits
Epitope AA 26-220
Reactivity Human
Kits with alternative reactivity to:
(19), (11), (10), (6), (5), (4), (3), (3), (3), (2), (2), (1)
Methode Type Sandwich ELISA
Detection Range 15.6-1000 pg/mL
Minimum Detection Limit 15.6 pg/mL
Application ELISA
Pubmed 3 references available
Supplier Log in to see

Product Details Oncostatin M ELISA Kit

Target details Application Details Handling References for Oncostatin M Kit (ABIN1672746) Images
Purpose For quantitative detection of human OSM in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant human OSM
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- human OSM antibod - 1
  • Lyophilized recombinant human OSM standar - 10ng/tubex2
  • Biotinylated anti- human OSM antibod - 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: A26R-220

Target details

Product Details Oncostatin M ELISA Kit Application Details Handling References for Oncostatin M Kit (ABIN1672746) Images back to top
Antigen
Alternative Name OSM (OSM ELISA Kit Abstract)
Background OSM(Oncostatin M) is a protein that in humans is encoded by the OSM gene. OSM is a pleiotropic cytokine that belongs to the interleukin 6 group of cytokines. The OSM gene is mapped on 22q12.2. Of these cytokines it most closely resembles leukemia inhibitory factor(LIF) in both structure and function. Treatment with recombinant OSM leads to the inhibition of proliferation and changes in cellular morphology of a number of tumor cell lines derived from a wide variety of tissue types. OSM signals through cell surface receptors that contain the protein gp130. The type I receptor is composed of gp130 and LIFR, the type II receptor is composed of gp130 and OSMR.
Gene ID 5008
UniProt P13725
Pathways JAK-STAT Signaling

Application Details

Product Details Oncostatin M ELISA Kit Target details Handling References for Oncostatin M Kit (ABIN1672746) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human OSM ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for OSM has been precoated onto 96-well plates. Standards(E.coli, A26R-220) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for OSM is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human OSM amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human OSM standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human OSM standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 104, Standard deviation: 5.72, CV(%): 5.5
  • Sample 2: n=16, Mean(pg/ml): 351, Standard deviation: 16.15, CV(%): 4.6
  • Sample 3: n=16, Mean(pg/ml): 627, Standard deviation: 42.64, CV(%): 6.8,
  • Sample 1: n=24, Mean(pg/ml): 135, Standard deviation: 8.37, CV(%): 6.2
  • Sample 2: n=24, Mean(pg/ml): 392, Standard deviation: 20.78, CV(%): 5.3
  • Sample 3: n=24, Mean(pg/ml): 658, Standard deviation: 47.4, CV(%): 7.2
Restrictions For Research Use only

Handling

Product Details Oncostatin M ELISA Kit Target details Application Details References for Oncostatin M Kit (ABIN1672746) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for Oncostatin M Kit (ABIN1672746)

Product Details Oncostatin M ELISA Kit Target details Application Details Handling Images back to top
Background publications

Tanaka, Miyajima: "Oncostatin M, a multifunctional cytokine." in: Reviews of physiology, biochemistry and pharmacology, Vol. 149, pp. 39-52, 2003

Auguste, Guillet, Fourcin, Olivier, Veziers, Pouplard-Barthelaix, Gascan: "Signaling of type II oncostatin M receptor." in: The Journal of biological chemistry, Vol. 272, Issue 25, pp. 15760-4, 1997

Rose, Bruce: "Oncostatin M is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 19, pp. 8641-5, 1991

Images

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Supplier Images
ELISA image for Oncostatin M ELISA Kit (OSM) (ABIN1672746) Human OSM/Oncostatin M PicoKine ELISA Kit standard curve