anti-Cow (Bovine) PIAS1 antibody for Western Blotting

Recommended PIAS1 Antibody (supplied by: Log in to see )

Antigen
Protein Inhibitor of Activated STAT, 1 (PIAS1) Antibodies
  • 2900068C24Rik
  • Ddxbp1
  • GBP
  • DDXBP1
  • GU/RH-II
  • ZMIZ3
  • ddxbp1
  • gbp
  • pias
  • pias1-A
  • xPAIS1
  • xPIAS1
  • zmiz3
  • protein inhibitor of activated STAT, 1
  • protein inhibitor of activated STAT 1
  • LOC100232805
  • Pias1
  • PIAS1
  • pias1
Epitope
N-Term
32
27
18
5
3
3
3
3
2
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
86
36
19
4
3
3
3
2
2
2
1
1
1
1
Host
Rabbit
81
8
Clonality
Polyclonal
Conjugate
This PIAS1 antibody is un-conjugated
4
4
4
4
4
4
Application
Western Blotting (WB)
84
56
51
18
8
6
5
3
2
2
1
1
Options
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Catalog No. ABIN2780997
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1873004 ABIN2780998 WB Rabbit Middle Region Log in to see Polyclonal 1
1 ABIN468444 WB Rabbit N-Term Log in to see Polyclonal
1 ABIN468445 WB Rabbit C-Term Log in to see Polyclonal

Top referenced anti-PIAS1 antibody for Western Blotting

Similar anti-PIAS1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Cow (Bovine) Dog (Canine) Guinea Pig Horse (Equine) Rabbit Zebrafish
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies 2 Antibodies 2 Antibodies
Western Blotting (WB) 82 Antibodies 36 Antibodies 20 Antibodies 4 Antibodies 3 Antibodies 2 Antibodies 5 Antibodies 3 Antibodies 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 6 Antibodies 2 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies 13 Antibodies 8 Antibodies 1 Antibodies

General

Antigen Protein Inhibitor of Activated STAT, 1 (PIAS1) Antibodies
Epitope N-Term
(32), (27), (18), (5), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
(86), (36), (19), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(81), (8)
Clonality
Polyclonal
Conjugate This PIAS1 antibody is un-conjugated
(4), (4), (4), (4), (4), (4)
Application Western Blotting (WB)
(84), (56), (51), (18), (8), (6), (5), (3), (2), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-PIAS1 Antibody

Target Details PIAS1 Application Details Handling ProductDetails: References for anti-PIAS1 antibody (ABIN2780997) Images
Blocking Peptide Blocking peptide for this product available: ABIN981725
Sequence MADSAELKQM VMSLRVSELQ VLLGYAGRNK HGRKHELLTK ALHLLKAGCS
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%
Characteristics This is a rabbit polyclonal antibody against PIAS1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human PIAS1

Target Details PIAS1

Product Details anti-PIAS1 Antibody Application Details Handling ProductDetails: References for anti-PIAS1 antibody (ABIN2780997) Images back to top
Antigen
Alternative Name PIAS1 (PIAS1 Antibody Abstract)
Background PIAS1 is a member of the mammalian PIAS [protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. It inhibits STAT1-mediated gene activation and the DNA binding activity, binds to Gu protein/RNA helicase II/DEAD box polypeptide 21, and interacts with androgen receptor (AR). It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis.This gene encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. It inhibits STAT1-mediated gene activation and the DNA binding activity, binds to Gu protein/RNA helicase II/DEAD box polypeptide 21, and interacts with androgen receptor (AR). It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: DDXBP1, GBP, GU/RH-II, MGC141878, MGC141879, ZMIZ3
Protein Interaction Partner: STAT1, TRIM63, TRIM55, CEP250, PRDM1, SUMO1, L3MBTL2, CBS, SHFM1, SUMO2, SUMO3, SOX2, CHUK, SPOP, YWHAZ, UBC, SKIL, SGTA, ATXN1, SMAD7, SMAD4, SMAD1, HTT, PRPF40A, PPP1CC, BARD1, PAXIP1, NR1H2, PPARGC1A, NR1H3, PPARGC1B, RAD54L2, ZMYND11, UBD, PLAG1, MYB,
Protein Size: 651
Molecular Weight 72 kDa
Gene ID 8554
NCBI Accession NM_016166, NP_057250
UniProt O75925
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Pathways JAK-STAT Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C

Application Details

Product Details anti-PIAS1 Antibody Target Details PIAS1 Handling ProductDetails: References for anti-PIAS1 antibody (ABIN2780997) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 651 AA

Restrictions For Research Use only

Handling

Product Details anti-PIAS1 Antibody Target Details PIAS1 Application Details ProductDetails: References for anti-PIAS1 antibody (ABIN2780997) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

ProductDetails: References for anti-PIAS1 antibody (ABIN2780997)

Product Details anti-PIAS1 Antibody Target Details PIAS1 Application Details Handling Images back to top
Background publications

Helbig, Yip, McCartney, Eyre, Beard: "A screening method for identifying disruptions in interferon signaling reveals HCV NS3/4a disrupts Stat-1 phosphorylation." in: Antiviral research, Vol. 77, Issue 3, pp. 169-76, 2008

Images

Product Details anti-PIAS1 Antibody Target Details PIAS1 Application Details Handling ProductDetails: References for anti-PIAS1 antibody (ABIN2780997) back to top
Supplier Images
Western Blotting (WB) image for anti-PIAS1 antibody (Protein Inhibitor of Activated STAT, 1) (N-Term) (ABIN2780997) anti-Protein Inhibitor of Activated STAT, 1 (PIAS1) (N-Term) antibody