You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human PIAS1 antibody for Immunocytochemistry

Recommended PIAS1 Antibody (supplied by: Log in to see )

Antigen
Protein Inhibitor of Activated STAT, 1 (PIAS1) Antibodies
  • 2900068C24Rik
  • DDXBP1
  • ddxbp1
  • Ddxbp1
  • GBP
  • gbp
  • GU/RH-II
  • pias
  • pias1-A
  • xPAIS1
  • xPIAS1
  • zmiz3
  • ZMIZ3
Epitope
AA 636-651, C-Term
34
29
17
5
3
3
2
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
91
32
17
2
2
1
1
1
Host
Rabbit
86
8
Clonality
Polyclonal
Conjugate
This PIAS1 antibody is un-conjugated
4
4
4
4
4
4
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
84
54
53
16
9
7
6
3
2
2
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN3044474
$ 200.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010194121 ABIN2626785 ICC IHC (p) WB Rabbit IgG AA 636-651 Log in to see Polyclonal
0.0010194121 ABIN1860208 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 409-645 Log in to see Polyclonal
0.0010194121 ABIN2737375 ICC FACS IP IHC WB Rabbit IgG Log in to see Polyclonal
0.0010194121 ABIN4345417 ELISA ICC IF IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.0010194121 ABIN2976281 ICC IF IHC WB Rabbit IgG Log in to see Polyclonal
0.0010194121 ABIN3032369 ICC IHC (p) WB Rabbit IgG AA 636-651 Log in to see Polyclonal
0.0010194121 ABIN3059655 ICC FACS IF IP IHC WB Rabbit Log in to see Polyclonal

Similar anti-PIAS1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
ELISA 9 Antibodies 14 Antibodies 53 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Flow Cytometry (FACS) 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 6 Antibodies 6 Antibodies
Immunofluorescence (IF) 6 Antibodies 7 Antibodies
Immunohistochemistry (IHC) 11 Antibodies 21 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 6 Antibodies 8 Antibodies
Immunoprecipitation (IP) 2 Antibodies 3 Antibodies
Western Blotting (WB) 18 Antibodies 33 Antibodies

General

Antigen Protein Inhibitor of Activated STAT, 1 (PIAS1) Antibodies
Epitope AA 636-651, C-Term
(34), (29), (17), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(91), (32), (17), (2), (2), (1), (1), (1)
Host Rabbit
(86), (8)
Clonality
Polyclonal
Conjugate This PIAS1 antibody is un-conjugated
(4), (4), (4), (4), (4), (4)
Application Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(84), (54), (53), (16), (9), (7), (6), (3), (2), (2), (1), (1)
Supplier Log in to see

Product Details anti-PIAS1 Antibody

Target Details PIAS1 Application Details Handling Images
Sequence DTASIFGIIP DIISLD
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for E3 SUMO-protein ligase PIAS1(PIAS1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: protein inhibitor of activated STAT, 1
Protein Name: E3 SUMO-protein ligase PIAS1
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human PIAS1(636-651aa DTASIFGIIPDIISLD), identical to the related mouse and rat sequences.
Isotype IgG

Target Details PIAS1

Product Details anti-PIAS1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PIAS1 (PIAS1 Antibody Abstract)
Background E3 SUMO-protein ligase PIAS1 is an enzyme that in humans is encoded by the PIAS1 gene. It is mapped to 15q23. This gene encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1(signal transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. PIAS1 inhibited STAT1-mediated gene activation in response to interferon when expressed in mammalian cells. It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis. On the other hand, PIAS1 also can function as a SUMO ligase, or possibly as a tightly bound regulator of it, toward p53.
UniProt O75925
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Pathways JAK-STAT Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-PIAS1 Antibody Target Details PIAS1 Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Predicted Species: Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions For Research Use only

Handling

Product Details anti-PIAS1 Antibody Target Details PIAS1 Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C,4 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Images

Product Details anti-PIAS1 Antibody Target Details PIAS1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-PIAS1 antibody (Protein Inhibitor of Activated STAT, 1) (AA 636-651) (ABIN3044474) Anti-PIAS1 antibody, IHC(P) IHC(P): Rat Testis Tissue
Immunohistochemistry (IHC) image for anti-PIAS1 antibody (Protein Inhibitor of Activated STAT, 1) (AA 636-651) (ABIN3044474) Anti-PIAS1 antibody, ICC ICC: A549 Cell
Immunohistochemistry (IHC) image for anti-PIAS1 antibody (Protein Inhibitor of Activated STAT, 1) (AA 636-651) (ABIN3044474) Anti-PIAS1 antibody, IHC(P) IHC(P): Human Placenta Tissue
Western Blotting (WB) image for anti-PIAS1 antibody (Protein Inhibitor of Activated STAT, 1) (AA 636-651) (ABIN3044474) anti-Protein Inhibitor of Activated STAT, 1 (PIAS1) (AA 636-651), (C-Term) antibody (Image 4)
Immunohistochemistry (IHC) image for anti-PIAS1 antibody (Protein Inhibitor of Activated STAT, 1) (AA 636-651) (ABIN3044474) Anti-PIAS1 antibody, IHC(P) IHC(P): Rat Brain Tissue
Immunohistochemistry (IHC) image for anti-PIAS1 antibody (Protein Inhibitor of Activated STAT, 1) (AA 636-651) (ABIN3044474) Anti-PIAS1 antibody, IHC(P) IHC(P): Rat Brain Tissue