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anti-Mouse (Murine) PIAS1 antibody for Immunofluorescence

Recommended PIAS1 Antibody (supplied by: Log in to see )

Antigen
Protein Inhibitor of Activated STAT, 1 (PIAS1) Antibodies
  • 2900068C24Rik
  • DDXBP1
  • ddxbp1
  • Ddxbp1
  • GBP
  • gbp
  • GU/RH-II
  • pias
  • pias1-A
  • xPAIS1
  • xPIAS1
  • zmiz3
  • ZMIZ3
Epitope
C-Term
34
28
17
5
3
3
3
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
89
30
16
2
2
1
1
1
Host
Rabbit
84
8
Clonality
Polyclonal
Conjugate
This PIAS1 antibody is un-conjugated
4
4
4
4
4
4
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
82
53
52
15
7
7
6
2
2
2
1
1
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Catalog No. ABIN500489
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010850558 ABIN2861223 IF IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.0010850558 ABIN2442929 IF IP IHC WB Rabbit C-Term Log in to see Polyclonal
0.0010850558 ABIN2959866 IF IHC ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.0010850558 ABIN2976281 ICC IF IHC WB Rabbit IgG Log in to see Polyclonal
0.0010850558 ABIN3059655 ICC FACS IF IP IHC WB Rabbit Log in to see Polyclonal

Top referenced anti-PIAS1 antibody for Immunofluorescence

Similar anti-PIAS1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
ELISA 8 Antibodies 13 Antibodies 52 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Flow Cytometry (FACS) 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 5 Antibodies
Immunofluorescence (IF) 5 Antibodies 6 Antibodies
Immunohistochemistry (IHC) 10 Antibodies 20 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies 7 Antibodies
Immunoprecipitation (IP) 2 Antibodies 3 Antibodies
Western Blotting (WB) 17 Antibodies 31 Antibodies

General

Antigen Protein Inhibitor of Activated STAT, 1 (PIAS1) Antibodies
Epitope C-Term
(34), (28), (17), (5), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(89), (30), (16), (2), (2), (1), (1), (1)
Host Rabbit
(84), (8)
Clonality
Polyclonal
Conjugate This PIAS1 antibody is un-conjugated
(4), (4), (4), (4), (4), (4)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(82), (53), (52), (15), (7), (7), (6), (2), (2), (2), (1), (1)
Pubmed 3 references available
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Product Details anti-PIAS1 Antibody

Target Details PIAS1 Application Details Handling References for anti-PIAS1 Antibody (ABIN500489) Images
Specificity This antibody is predicted to not cross-react with other members of the PIAS family.
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse, Rat.
Purification Affinity chromatography purified via peptide column
Immunogen 15 amino acid peptide near the carboxy terminus of Human PIAS1.
Isotype IgG

Target Details PIAS1

Product Details anti-PIAS1 Antibody Application Details Handling References for anti-PIAS1 Antibody (ABIN500489) Images back to top
Antigen
Alternative Name PIAS1 (PIAS1 Antibody Abstract)
Background The PIAS proteins (protein inhibitor of activated STAT) play a crucial role as transcriptional coregulators in various cellular pathways, including the STAT, p53 and the steroid hormone signaling pathway. The PIAS protein family includes at least five evolutionarily conserved genes, including PIAS1. The major function of the PIAS proteins is the control of gene transcription and can also act as small ubiquitin-like-modifier (SUMO) E3 ligases. PIAS1 binds specifically to STAT1, inhibiting STAT1-mediated gene activation and also binds to the Gu/RNA helicase II enzyme, leading to the proteolytic cleavage of Gu/RH-II. PIAS1 is a potent co-activator for CP2c-mediated ?-globin expression in erythroid cells.Synonyms: DDXBP1, DEAD/H box-binding protein 1, E3 SUMO-protein ligase PIAS1, Gu-binding protein, PIAS-1, Protein inhibitor of activated STAT protein 1, RNA helicase II-binding protein
Gene ID 8554
NCBI Accession NP_057250
UniProt O75925
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Pathways JAK-STAT Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-PIAS1 Antibody Target Details PIAS1 Handling References for anti-PIAS1 Antibody (ABIN500489) Images back to top
Application Notes ELISA. Western blot: 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PIAS1 Antibody Target Details PIAS1 Application Details References for anti-PIAS1 Antibody (ABIN500489) Images back to top
Concentration 1.0 mg/mL
Buffer PBS containing 0.02 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-PIAS1 Antibody (ABIN500489)

Product Details anti-PIAS1 Antibody Target Details PIAS1 Application Details Handling Images back to top
Background publications

Kang, Chae, Jeon, Kim, Ha, Shin, Kim, Kim: "PIAS1 regulates CP2c localization and active promoter complex formation in erythroid cell-specific alpha-globin expression." in: Nucleic acids research, Vol. 38, Issue 16, pp. 5456-71, 2010

Shuai, Liu: "Regulation of gene-activation pathways by PIAS proteins in the immune system." in: Nature reviews. Immunology, Vol. 5, Issue 8, pp. 593-605, 2005

Liu, Liao, Rao, Kushner, Chung, Chang, Shuai: "Inhibition of Stat1-mediated gene activation by PIAS1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 18, pp. 10626-31, 1998

Images

Product Details anti-PIAS1 Antibody Target Details PIAS1 Application Details Handling References for anti-PIAS1 Antibody (ABIN500489) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-PIAS1 antibody (Protein Inhibitor of Activated STAT, 1) (C-Term) (ABIN500489) Immunohistochemistry of PIAS1 in rat kidney tissue with PIAS1 Antibody  at 5 μg/ml
Western Blotting (WB) image for anti-PIAS1 antibody (Protein Inhibitor of Activated STAT, 1) (C-Term) (ABIN500489) Western blot analysis of PIAS1 in human kidney tissue lysate with PIAS1 antibody at 1...