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anti-Mouse (Murine) PIAS1 antibody for Western Blotting

Recommended PIAS1 Antibody (supplied by: Log in to see )

Antigen
Protein Inhibitor of Activated STAT, 1 (PIAS1) Antibodies
  • 2900068C24Rik
  • DDXBP1
  • ddxbp1
  • Ddxbp1
  • GBP
  • gbp
  • GU/RH-II
  • pias
  • pias1-A
  • xPAIS1
  • xPIAS1
  • zmiz3
  • ZMIZ3
Epitope
N-Term
33
30
17
5
3
3
3
2
2
2
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
92
33
17
1
1
Host
Rabbit
87
8
Clonality
Polyclonal
Conjugate
This PIAS1 antibody is un-conjugated
4
4
4
4
4
4
Application
Western Blotting (WB)
85
55
53
18
9
8
6
3
2
2
1
1
Supplier
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Catalog No. ABIN2780997
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13341247 ABIN500489 EIA IF IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 3
0.13341247 ABIN1533432 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN3044474 ICC IHC (p) WB Rabbit IgG AA 636-651, C-Term Log in to see Polyclonal
0.13341247 ABIN615743 EIA IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.13341247 ABIN2499373 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN3186436 ELISA IHC WB Rabbit IgG N-Term Log in to see Polyclonal
0.13341247 ABIN1030583 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.13341247 ABIN2432537 ELISA WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN2499375 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.13341247 ABIN1848832 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.0011728023 ABIN271951 IHC (p) WB Rabbit Log in to see Polyclonal
0.0011728023 ABIN1738080 IHC (p) ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.0011728023 ABIN2957720 IHC WB Rabbit N-Term Log in to see Polyclonal
0.0011728023 ABIN2737375 ICC FACS IP IHC WB Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN407959 IHC (p) IHC WB Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN2861223 IF IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.0011728023 ABIN1887056 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.0011728023 ABIN2564529 IHC WB Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN1585043 IHC ELISA WB Rabbit Log in to see Polyclonal
0.0011728023 ABIN2442926 WB Mouse C-Term Log in to see

Top referenced anti-PIAS1 antibody for Western Blotting

Similar anti-PIAS1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Chicken Cow (Bovine) Dog (Canine) Xenopus laevis Zebrafish (Danio rerio)
Western Blotting (WB) 83 Antibodies 33 Antibodies 18 Antibodies 1 Antibodies 2 Antibodies 2 Antibodies 1 Antibodies 1 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 6 Antibodies 3 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies 8 Antibodies 6 Antibodies
Immunohistochemistry (IHC) 55 Antibodies 21 Antibodies 11 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunofluorescence (IF) 9 Antibodies

General

Antigen Protein Inhibitor of Activated STAT, 1 (PIAS1) Antibodies
Epitope N-Term
(33), (30), (17), (5), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(92), (33), (17), (1), (1)
Host Rabbit
(87), (8)
Clonality
Polyclonal
Conjugate This PIAS1 antibody is un-conjugated
(4), (4), (4), (4), (4), (4)
Application Western Blotting (WB)
(85), (55), (53), (18), (9), (8), (6), (3), (2), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-PIAS1 Antibody

Target Details PIAS1 Application Details Handling References for anti-PIAS1 Antibody (ABIN2780997) Images
Blocking Peptide Blocking peptide for this product available: ABIN981725
Sequence MADSAELKQM VMSLRVSELQ VLLGYAGRNK HGRKHELLTK ALHLLKAGCS
Predicted Reactivity African clawed frog : 100 %, Bovine : 100 %, Chicken : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %
Characteristics This is a rabbit polyclonal antibody against PIAS1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human PIAS1

Target Details PIAS1

Product Details anti-PIAS1 Antibody Application Details Handling References for anti-PIAS1 Antibody (ABIN2780997) Images back to top
Antigen
Alternative Name PIAS1 (PIAS1 Antibody Abstract)
Background PIAS1 is a member of the mammalian PIAS [protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. It inhibits STAT1-mediated gene activation and the DNA binding activity, binds to Gu protein/RNA helicase II/DEAD box polypeptide 21, and interacts with androgen receptor (AR). It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis.This gene encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. It inhibits STAT1-mediated gene activation and the DNA binding activity, binds to Gu protein/RNA helicase II/DEAD box polypeptide 21, and interacts with androgen receptor (AR). It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis.
Molecular Weight 72 kDa
Gene ID 8554
NCBI Accession NM_016166, NP_057250
UniProt O75925
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Pathways JAK-STAT Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-PIAS1 Antibody Target Details PIAS1 Handling References for anti-PIAS1 Antibody (ABIN2780997) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 651 AA

Restrictions For Research Use only

Handling

Product Details anti-PIAS1 Antibody Target Details PIAS1 Application Details References for anti-PIAS1 Antibody (ABIN2780997) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-PIAS1 Antibody (ABIN2780997)

Product Details anti-PIAS1 Antibody Target Details PIAS1 Application Details Handling Images back to top
Background publications

Helbig, Yip, McCartney, Eyre, Beard: "A screening method for identifying disruptions in interferon signaling reveals HCV NS3/4a disrupts Stat-1 phosphorylation." in: Antiviral research, Vol. 77, Issue 3, pp. 169-76, 2008

Images

Product Details anti-PIAS1 Antibody Target Details PIAS1 Application Details Handling References for anti-PIAS1 Antibody (ABIN2780997) back to top
Supplier Images
Western Blotting (WB) image for anti-PIAS1 antibody (Protein Inhibitor of Activated STAT, 1) (N-Term) (ABIN2780997) anti-Protein Inhibitor of Activated STAT, 1 (PIAS1) (N-Term) antibody