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anti-Human PIAS2 antibody for Enzyme Immunoassay

Recommended PIAS2 Antibody (supplied by: Log in to see )

Antigen
Protein Inhibitor of Activated STAT, 2 (PIAS2) Antibodies
  • 6330408K17Rik
  • AI462206
  • ARIP3
  • AU018068
  • Dib
  • DIP
  • MGC83751
  • MIZ
  • MIZ1
  • Miz1
  • PIAS2
  • piasx
  • PIASX
  • PIASX-ALPHA
  • PIASX-BETA
  • PIASxalpha
  • PIASxb
  • PIASxbeta
  • SIZ2
  • wu:fi05f09
  • ZMIZ4
Epitope
C-Term
30
22
19
5
3
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
120
50
37
14
6
4
2
2
2
1
1
Host
Rabbit
84
32
7
2
Clonality
Polyclonal
Conjugate
This PIAS2 antibody is un-conjugated
4
4
4
4
4
4
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
96
68
48
14
11
3
3
2
1
1
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Catalog No. ABIN356767
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN783596 EIA WB Rabbit N-Term Log in to see Polyclonal 1
0.0008867729 ABIN614937 EIA IHC (p) Goat Internal Region Log in to see Polyclonal
0.0008867729 ABIN615949 EIA WB Goat Internal Region, AA 185-196 Log in to see Polyclonal

Top referenced anti-PIAS2 antibody for Enzyme Immunoassay

Similar anti-PIAS2 Antibodies

Application / Reactivity Human
ELISA 68 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 14 Antibodies
Immunohistochemistry (IHC) 47 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 13 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
RNA Interference (RNAi) 1 Antibodies
Western Blotting (WB) 92 Antibodies

General

Antigen Protein Inhibitor of Activated STAT, 2 (PIAS2) Antibodies
Epitope C-Term
(30), (22), (19), (5), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(120), (50), (37), (14), (6), (4), (2), (2), (2), (1), (1)
Host Rabbit
(84), (32), (7), (2)
Clonality
Polyclonal
Conjugate This PIAS2 antibody is un-conjugated
(4), (4), (4), (4), (4), (4)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(96), (68), (48), (14), (11), (3), (3), (2), (1), (1)
Pubmed 5 references available
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Product Details anti-PIAS2 Antibody

Target Details PIAS2 Application Details Handling References for anti-PIAS2 Antibody (ABIN356767) Images
Specificity This antibody is specific to PIAS2 (PIASx1/2) (C-term).
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PIASx1/2.
Isotype Ig

Target Details PIAS2

Product Details anti-PIAS2 Antibody Application Details Handling References for anti-PIAS2 Antibody (ABIN356767) Images back to top
Antigen
Alternative Name PIAS2 (PIAS2 Antibody Abstract)
Background PIASX functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. This protein plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. PIASX binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha.Synonyms: ARIP3, Androgen receptor-interacting protein 3, DAB2-interacting protein, DIP, E3 SUMO-protein ligase PIAS2, Miz1, Msx-interacting zinc finger protein, PIAS-NY protein, PIASX, Protein inhibitor of activated STAT x, Protein inhibitor of activated STAT2
Gene ID 9063, 9606
UniProt O75928
Research Area Chromatin and Nuclear Signaling, Proteolysis / Ubiquitin, Transcription Factors
Pathways JAK-STAT Signaling

Application Details

Product Details anti-PIAS2 Antibody Target Details PIAS2 Handling References for anti-PIAS2 Antibody (ABIN356767) Images back to top
Application Notes ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PIAS2 Antibody Target Details PIAS2 Application Details References for anti-PIAS2 Antibody (ABIN356767) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-PIAS2 Antibody (ABIN356767)

Product Details anti-PIAS2 Antibody Target Details PIAS2 Application Details Handling Images back to top
Background publications

Sakurai, Itoh, Higashitsuji, Nagao, Nonoguchi, Chiba, Fujita: "A cleaved form of MAGE-A4 binds to Miz-1 and induces apoptosis in human cells." in: The Journal of biological chemistry, Vol. 279, Issue 15, pp. 15505-14, 2004

Arora, Liu, He, Kim, Murphy, Murphy, Modlin, Shuai: "PIASx is a transcriptional co-repressor of signal transducer and activator of transcription 4." in: The Journal of biological chemistry, Vol. 278, Issue 24, pp. 21327-30, 2003

Wu, Cetinkaya, Munoz-Alonso, von der Lehr, Bahram, Beuger, Eilers, Leon, Larsson: "Myc represses differentiation-induced p21CIP1 expression via Miz-1-dependent interaction with the p21 core promoter." in: Oncogene, Vol. 22, Issue 3, pp. 351-60, 2003

Liu, Liao, Rao, Kushner, Chung, Chang, Shuai: "Inhibition of Stat1-mediated gene activation by PIAS1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 18, pp. 10626-31, 1998

Wu, Wu, Ma, Sangiorgi, Wu, Bell, Lyons, Maxson: "Miz1, a novel zinc finger transcription factor that interacts with Msx2 and enhances its affinity for DNA." in: Mechanisms of development, Vol. 65, Issue 1-2, pp. 3-17, 1997

Images

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Supplier Images
 image for anti-PIAS2 antibody (Protein Inhibitor of Activated STAT, 2) (C-Term) (ABIN356767) anti-Protein Inhibitor of Activated STAT, 2 (PIAS2) (C-Term) antibody
 image for anti-PIAS2 antibody (Protein Inhibitor of Activated STAT, 2) (C-Term) (ABIN356767) anti-Protein Inhibitor of Activated STAT, 2 (PIAS2) (C-Term) antibody (Image 2)