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anti-Human PIAS2 antibody for Western Blotting

Recommended PIAS2 Antibody (supplied by: Log in to see )

Antigen
Protein Inhibitor of Activated STAT, 2 (PIAS2) Antibodies
  • 6330408K17Rik
  • AI462206
  • ARIP3
  • AU018068
  • Dib
  • DIP
  • MGC83751
  • MIZ
  • MIZ1
  • Miz1
  • PIAS2
  • piasx
  • PIASX
  • PIASX-ALPHA
  • PIASX-BETA
  • PIASxalpha
  • PIASxb
  • PIASxbeta
  • SIZ2
  • wu:fi05f09
  • ZMIZ4
Epitope
N-Term
31
19
18
5
3
2
2
2
1
1
1
1
1
1
Reactivity
Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
112
44
32
13
6
3
2
2
2
1
1
Host
Rabbit
78
31
6
2
Clonality
Polyclonal
Conjugate
This PIAS2 antibody is un-conjugated
4
4
4
4
4
4
Application
Western Blotting (WB)
89
64
45
12
9
4
2
1
1
1
Supplier
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Catalog No. ABIN2785610
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12303223 ABIN2776422 WB Rabbit C-Term Log in to see Polyclonal 1
0.12303223 ABIN2776423 WB Rabbit C-Term Log in to see Polyclonal 1
0.12303223 ABIN783596 EIA WB Rabbit N-Term Log in to see Polyclonal 1
0.12303223 ABIN2779873 IHC WB Rabbit C-Term Log in to see Polyclonal
0.12303223 ABIN615949 EIA WB Goat Internal Region, AA 185-196 Log in to see Polyclonal
0.12303223 ABIN1533433 ELISA WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN2707702 WB Rabbit N-Term Log in to see Polyclonal
0.12303223 ABIN2461199 ELISA WB Rabbit Log in to see Polyclonal
0.12303223 ABIN2461198 ELISA WB Rabbit Log in to see Polyclonal
0.12303223 ABIN2785609 WB Rabbit N-Term Log in to see Polyclonal
0.12303223 ABIN2463619 ELISA WB Rabbit Log in to see Polyclonal
0.12303223 ABIN1876855 IHC WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN2499378 ELISA WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN948767 IP WB Rabbit AA 1-572, full length Log in to see Polyclonal
0.12303223 ABIN563927 IF PLA RNAi ELISA WB Mouse IgG2a kappa AA 385-473, partial Log in to see 1F7
0.12303223 ABIN3186438 ELISA WB Rabbit IgG N-Term Log in to see Polyclonal
0.12303223 ABIN522503 PLA WB Rabbit AA 1-572, full length Log in to see Polyclonal
0.12303223 ABIN522502 IF WB Mouse AA 1-572, full length Log in to see Polyclonal
0.12303223 ABIN1031511 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.12303223 ABIN2406426 IF IHC WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-PIAS2 antibody for Western Blotting

Similar anti-PIAS2 Antibodies

Application / Reactivity Human Chicken Dog (Canine) Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 86 Antibodies 3 Antibodies 8 Antibodies 38 Antibodies 26 Antibodies
RNA Interference (RNAi) 1 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 10 Antibodies 3 Antibodies 3 Antibodies 3 Antibodies
Immunohistochemistry (IHC) 44 Antibodies 15 Antibodies 3 Antibodies 12 Antibodies
Immunofluorescence (IF) 12 Antibodies 4 Antibodies 4 Antibodies
Immunocytochemistry (ICC) 1 Antibodies

General

Antigen Protein Inhibitor of Activated STAT, 2 (PIAS2) Antibodies
Epitope N-Term
(31), (19), (18), (5), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(112), (44), (32), (13), (6), (3), (2), (2), (2), (1), (1)
Host Rabbit
(78), (31), (6), (2)
Clonality
Polyclonal
Conjugate This PIAS2 antibody is un-conjugated
(4), (4), (4), (4), (4), (4)
Application Western Blotting (WB)
(89), (64), (45), (12), (9), (4), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-PIAS2 Antibody

Target Details PIAS2 Application Details Handling References for anti-PIAS2 Antibody (ABIN2785610) Images
Blocking Peptide Blocking peptide for this product available: ABIN981732
Sequence RELYRRRYPR TLEGLSDLST IKSSVFSLDG GSSPVEPDLA VAGIHSLPST
Predicted Reactivity Human : 100 %, Bovine : 90 %, Dog : 90 %, Guinea pig : 90 %, Horse : 90 %, Mouse : 90 %, Rabbit : 90 %, Rat : 90 %, Chicken : 80 %
Characteristics This is a rabbit polyclonal antibody against PIAS2. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human PIAS2

Target Details PIAS2

Product Details anti-PIAS2 Antibody Application Details Handling References for anti-PIAS2 Antibody (ABIN2785610) Images back to top
Antigen
Alternative Name PIAS2 (PIAS2 Antibody Abstract)
Background Pias2 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. It plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. It seems to be mostly involved in gene silencing. It binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. This gene encodes a protein involved in the regulation of transcription factors involved in MAP kinase signaling. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1. Two alternatively spliced transcripts encoding different isoforms have been described.
Molecular Weight 63 kDa
Gene ID 9063
NCBI Accession NP_775298, NM_173206
Research Area Chromatin and Nuclear Signaling, Proteolysis / Ubiquitin, Transcription Factors
Pathways JAK-STAT Signaling

Application Details

Product Details anti-PIAS2 Antibody Target Details PIAS2 Handling References for anti-PIAS2 Antibody (ABIN2785610) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 572 AA

Restrictions For Research Use only

Handling

Product Details anti-PIAS2 Antibody Target Details PIAS2 Application Details References for anti-PIAS2 Antibody (ABIN2785610) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-PIAS2 Antibody (ABIN2785610)

Product Details anti-PIAS2 Antibody Target Details PIAS2 Application Details Handling Images back to top
Background publications

Tsang, Connell, Brown, Thompson, Reid, Sanderson: "A systematic analysis of human CHMP protein interactions: additional MIT domain-containing proteins bind to multiple components of the human ESCRT III complex." in: Genomics, Vol. 88, Issue 3, pp. 333-46, 2006

Images

Product Details anti-PIAS2 Antibody Target Details PIAS2 Application Details Handling References for anti-PIAS2 Antibody (ABIN2785610) back to top
Supplier Images
Western Blotting (WB) image for anti-PIAS2 antibody (Protein Inhibitor of Activated STAT, 2) (N-Term) (ABIN2785610) WB Suggested Anti-PIAS2 Antibody Titration: 0.2-1 ug/ml
Positive Control...