anti-Rat (Rattus) PIAS2 antibody for Western Blotting

Recommended PIAS2 Antibody (supplied by: Log in to see )

Antigen
Protein Inhibitor of Activated STAT, 2 (PIAS2) Antibodies
  • MGC83751
  • piasx
  • wu:fi05f09
  • PIAS2
  • 6330408K17Rik
  • AI462206
  • ARIP3
  • AU018068
  • Dib
  • Miz1
  • PIASxalpha
  • PIASxb
  • PIASxbeta
  • SIZ2
  • DIP
  • MIZ
  • MIZ1
  • PIASX
  • PIASX-ALPHA
  • PIASX-BETA
  • ZMIZ4
  • protein inhibitor of activated STAT, 2
  • protein inhibitor of activated STAT 2
  • pias2
  • PIAS2
  • Pias2
Epitope
N-Term
29
18
17
5
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
123
53
45
21
14
11
11
5
4
4
2
1
1
Host
Rabbit
83
34
7
2
Clonality
Polyclonal
Conjugate
This PIAS2 antibody is un-conjugated
4
4
4
4
4
4
Application
Western Blotting (WB)
100
69
49
14
14
4
3
2
1
1
Options
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Catalog No. ABIN2785610
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.2182703 ABIN2780293 WB Rabbit N-Term Log in to see Polyclonal
3.2182703 ABIN2785609 WB Rabbit N-Term Log in to see Polyclonal
3.2182703 ABIN1533433 ELISA WB Rabbit IgG AA 10-59 Log in to see Polyclonal
3.2182703 ABIN3186438 ELISA WB Rabbit IgG N-Term Log in to see Polyclonal
3.2182703 ABIN615949 EIA WB Goat Internal Region, AA 185-196 Log in to see Polyclonal
3.2182703 ABIN2707702 WB Rabbit N-Term Log in to see Polyclonal
3.2182703 ABIN407960 WB Rabbit IgG Log in to see Polyclonal
3.2182703 ABIN1876855 IF IHC WB Rabbit IgG Log in to see Polyclonal
3.2182703 ABIN4345422 ELISA ICC IF IHC IHC (p) WB Rabbit N-Term Log in to see Polyclonal
3.2182703 ABIN2564531 ELISA WB Goat IgG AA 185-196 Log in to see Polyclonal
3.2182703 ABIN2406426 IF IHC WB Rabbit IgG Log in to see Polyclonal
3.2182703 ABIN1031511 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
3.2182703 ABIN1848834 ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN202826 WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN202828 WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN490650 ELISA WB Goat AA 185-196 Log in to see Polyclonal
1 ABIN5554353 EIA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN216786 IHC WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN799244 WB Rabbit AA 51-100 Log in to see Polyclonal
1 ABIN271952 WB Rabbit Log in to see Polyclonal

General

Antigen Protein Inhibitor of Activated STAT, 2 (PIAS2) Antibodies
Epitope N-Term
(29), (18), (17), (5), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(123), (53), (45), (21), (14), (11), (11), (5), (4), (4), (2), (1), (1)
Host Rabbit
(83), (34), (7), (2)
Clonality
Polyclonal
Conjugate This PIAS2 antibody is un-conjugated
(4), (4), (4), (4), (4), (4)
Application Western Blotting (WB)
(100), (69), (49), (14), (14), (4), (3), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-PIAS2 Antibody

Target Details PIAS2 Application Details Handling References for anti-PIAS2 antibody (ABIN2785610) Images
Blocking Peptide Blocking peptide for this product available: ABIN981732
Sequence RELYRRRYPR TLEGLSDLST IKSSVFSLDG GSSPVEPDLA VAGIHSLPST
Predicted Reactivity Cow: 93%, Dog: 92%, Guinea Pig: 92%, Horse: 92%, Human: 100%, Mouse: 92%, Pig: 93%, Rabbit: 92%, Rat: 93%
Characteristics This is a rabbit polyclonal antibody against PIAS2. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human PIAS2

Target Details PIAS2

Product Details anti-PIAS2 Antibody Application Details Handling References for anti-PIAS2 antibody (ABIN2785610) Images back to top
Antigen
Alternative Name PIAS2 (PIAS2 Antibody Abstract)
Background Pias2 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. It plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. It seems to be mostly involved in gene silencing. It binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. This gene encodes a protein involved in the regulation of transcription factors involved in MAP kinase signaling. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1. Two alternatively spliced transcripts encoding different isoforms have been described.
Alias Symbols: MGC102682, MIZ1, PIASX-ALPHA, PIASX-BETA, SIZ2, ZMIZ4, miz, DIP, MIZ, ARIP3, PIASX
Protein Interaction Partner: HIC1, GOLGA2, GFAP, PHC1, TRIM23, ZBTB8A, SUMO1P1, ACY3, TSR2, NAV2, CCHCR1, CCNDBP1, MAPKAPK2, ZBED1, PIAS2, SUMO1, UBE2I, TP53, PRKAB2, MX2, TRIM63, TRIM55, CTBP1, GTF2I, SUMO2, SUMO3, MYC, DNMT3A, TICAM2, RUFY1, PIAS4, C1QA, ADA, PAXIP1, UBC, PLAGL1, P
Protein Size: 572
Molecular Weight 63 kDa
Gene ID 9063
NCBI Accession NM_173206, NP_775298
Research Area Chromatin and Nuclear Signaling, Proteolysis / Ubiquitin, Transcription Factors
Pathways JAK-STAT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling

Application Details

Product Details anti-PIAS2 Antibody Target Details PIAS2 Handling References for anti-PIAS2 antibody (ABIN2785610) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 572 AA

Restrictions For Research Use only

Handling

Product Details anti-PIAS2 Antibody Target Details PIAS2 Application Details References for anti-PIAS2 antibody (ABIN2785610) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-PIAS2 antibody (ABIN2785610)

Product Details anti-PIAS2 Antibody Target Details PIAS2 Application Details Handling Images back to top
Background publications

Tsang, Connell, Brown, Thompson, Reid, Sanderson: "A systematic analysis of human CHMP protein interactions: additional MIT domain-containing proteins bind to multiple components of the human ESCRT III complex." in: Genomics, Vol. 88, Issue 3, pp. 333-46, 2006

Images

Product Details anti-PIAS2 Antibody Target Details PIAS2 Application Details Handling References for anti-PIAS2 antibody (ABIN2785610) back to top
Supplier Images
Western Blotting (WB) image for anti-Protein Inhibitor of Activated STAT, 2 (PIAS2) (N-Term) antibody (ABIN2785610) WB Suggested Anti-PIAS2 Antibody Titration: 0.2-1 ug/ml
Positive Control...