anti-Human PIAS3 antibody for Western Blotting

Recommended PIAS3 Antibody (supplied by: Log in to see )

Antigen
Protein Inhibitor of Activated STAT, 3 (PIAS3) Antibodies
  • PIAS3
  • Pias3l
  • ZMIZ5
  • protein inhibitor of activated STAT, 3
  • protein inhibitor of activated STAT 3
  • PIAS3
  • pias3
  • Pias3
  • LOC100341019
Epitope
C-Term
15
11
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
66
30
27
7
6
4
3
2
2
1
Host
Rabbit
62
4
Clonality
Polyclonal
Application
Immunohistochemistry (IHC), Western Blotting (WB)
59
35
31
15
6
2
2
2
1
1
Options
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Catalog No. ABIN2777574
$ 229.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9476938 ABIN2777575 WB Rabbit Middle Region Log in to see Polyclonal 1
2.9476938 ABIN1882115 IHC (p) WB Rabbit AA 588-619, C-Term Log in to see Polyclonal 7
2.9476938 ABIN1882116 IHC (p) WB Rabbit AA 95-126, N-Term Log in to see Polyclonal 7
2.9476938 ABIN1533434 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
2.9476938 ABIN3186437 ELISA IHC WB Rabbit IgG N-Term Log in to see Polyclonal
2.9476938 ABIN2707703 IHC WB Rabbit N-Term Log in to see Polyclonal
2.9476938 ABIN783253 EIA IHC (p) WB Rabbit C-Term Log in to see Polyclonal
2.9476938 ABIN2460563 IHC ELISA WB Rabbit Log in to see Polyclonal
2.9476938 ABIN4345426 ELISA IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal
2.9476938 ABIN2564532 IF IHC WB Rabbit IgG Log in to see Polyclonal
2.9476938 ABIN564520 RNAi ELISA WB Mouse IgG2a kappa AA 453-550, partial Log in to see 4F12
2.9476938 ABIN1030584 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
2.9476938 ABIN1848836 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN202823 IHC (p) WB Rabbit IgG AA 548-597 Log in to see Polyclonal
1 ABIN5555120 EIA WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN467799 WB Rabbit AA 395-444 Log in to see Polyclonal
1 ABIN271953 IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN2879031 IHC (p) ELISA WB Rabbit IgG AA 10-59 Log in to see Polyclonal
1 ABIN605174 IHC (p) ELISA WB Rabbit C-Term Log in to see Polyclonal
1 ABIN1738082 IHC (p) ELISA WB Rabbit IgG C-Term Log in to see Polyclonal

General

Antigen Protein Inhibitor of Activated STAT, 3 (PIAS3) Antibodies
Epitope C-Term
(15), (11), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(66), (30), (27), (7), (6), (4), (3), (2), (2), (1)
Host Rabbit
(62), (4)
Clonality
Polyclonal
Application Immunohistochemistry (IHC), Western Blotting (WB)
(59), (35), (31), (15), (6), (2), (2), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-PIAS3 Antibody

Target Details PIAS3 Application Details Handling ProductDetails: References for anti-PIAS3 antibody (ABIN2777574) Images
Sequence TSLDEQDALG HFFQYRGTPS HFLGPLAPTL GSSHCSATPA PPPGRVSSIV
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 93%, Pig: 100%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against PIAS3. It was validated on Western Blot and immunohistochemistry.
Purification Protein A purified
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human PIAS3

Target Details PIAS3

Product Details anti-PIAS3 Antibody Application Details Handling ProductDetails: References for anti-PIAS3 antibody (ABIN2777574) Images back to top
Antigen
Alternative Name PIAS3 (PIAS3 Antibody Abstract)
Background PIAS3 is a member of the protein inhibitor of activated STAT (PIAS) family. It also activates TGF-beta/SMAD transcriptional responses.
Alias Symbols: ZMIZ5
Protein Interaction Partner: UBC, HIC1, TRIM63, TRIM55, TAB2, SUMO1, CBS, SUMO2, SUMO3, STAT3, NCOA2, CREM, PRPF40A, SERPINA10, GEMIN4, UBE2I, SKIL, HNRNPK, ZFHX3, SERBP1, SATB1, ERBB4, Bcl11a, ELAVL1, PPP1CA, Grm7, Grm8, Grm6, Grm4, TRIM8, RAC1, C19orf60, CARHSP1, UBA1, PSMC1, TRIM2
Protein Size: 619
Molecular Weight 67 kDa
Gene ID 10401
NCBI Accession NM_006099, NP_006090
UniProt Q9Y6X2
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Pathways JAK-STAT Signaling

Application Details

Product Details anti-PIAS3 Antibody Target Details PIAS3 Handling ProductDetails: References for anti-PIAS3 antibody (ABIN2777574) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 619 AA

Restrictions For Research Use only

Handling

Product Details anti-PIAS3 Antibody Target Details PIAS3 Application Details ProductDetails: References for anti-PIAS3 antibody (ABIN2777574) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

ProductDetails: References for anti-PIAS3 antibody (ABIN2777574)

Product Details anti-PIAS3 Antibody Target Details PIAS3 Application Details Handling Images back to top
Background publications

Jang, Yoon, Shin, Kim, Lee: "PIAS3 suppresses NF-kappaB-mediated transcription by interacting with the p65/RelA subunit." in: The Journal of biological chemistry, Vol. 279, Issue 23, pp. 24873-80, 2004

Images

Product Details anti-PIAS3 Antibody Target Details PIAS3 Application Details Handling ProductDetails: References for anti-PIAS3 antibody (ABIN2777574) back to top
Supplier Images
Western Blotting (WB) image for anti-Protein Inhibitor of Activated STAT, 3 (PIAS3) (C-Term) antibody (ABIN2777574) WB Suggested Anti-PIAS3 Antibody Titration: 1.0-2.0ug/ml
Positive Contro...
Immunohistochemistry (IHC) image for anti-Protein Inhibitor of Activated STAT, 3 (PIAS3) (C-Term) antibody (ABIN2777574) anti-Protein Inhibitor of Activated STAT, 3 (PIAS3) (C-Term) antibody (Image 2)