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anti-Mouse (Murine) Prolactin antibody for Immunocytochemistry

Recommended Prolactin Antibody (supplied by: Log in to see )

Antigen
Prolactin (PRL) Antibodies
  • AV290867
  • prl
  • PRL
  • Prl1a1
  • PRLB
  • PRLSD1
  • Prol
  • prolactin
  • RATPRLSD1
  • RNPROL
Epitope
AA 1-226
23
22
20
16
14
14
13
11
10
10
9
8
7
7
6
6
5
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
445
71
48
13
13
3
2
2
1
1
1
1
Host
Rabbit
295
225
5
1
1
Clonality
Polyclonal
Conjugate
This Prolactin antibody is un-conjugated
35
29
29
24
21
21
4
4
3
3
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
358
186
80
64
41
38
28
27
27
24
12
11
8
8
8
5
4
4
2
2
2
2
2
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN1078459
$ 356.40
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0009962841 ABIN2751183 CM ICC IF IHC ELISA WB FITC Rabbit IgG AA 1-100, N-Term Log in to see Polyclonal
0.0009962841 ABIN2751184 CM ICC IF IHC ELISA WB FITC Rabbit IgG AA 100-200, C-Term Log in to see Polyclonal
0.0009962841 ABIN3209809 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal
0.0009962841 ABIN2747274 CM ICC IF IHC ELISA WB Rabbit IgG AA 1-100, N-Term Log in to see Polyclonal
0.0009962841 ABIN2747275 CM ICC IF IHC ELISA WB Rabbit IgG AA 100-200, C-Term Log in to see Polyclonal

Similar anti-Prolactin Antibodies

Application / Reactivity Mouse (Murine)
Confocal Microscopy (CM) 4 Antibodies
ELISA 21 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 4 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 13 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Neutralization (Neut) 1 Antibodies
Western Blotting (WB) 35 Antibodies

General

Antigen Prolactin (PRL) Antibodies
Epitope AA 1-226
(23), (22), (20), (16), (14), (14), (13), (11), (10), (10), (9), (8), (7), (7), (6), (6), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(445), (71), (48), (13), (13), (3), (2), (2), (1), (1), (1), (1)
Host Rabbit
(295), (225), (5), (1), (1)
Clonality
Polyclonal
Conjugate This Prolactin antibody is un-conjugated
(35), (29), (29), (24), (21), (21), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(358), (186), (80), (64), (41), (38), (28), (27), (27), (24), (12), (11), (8), (8), (8), (5), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-Prolactin Antibody

Target Details Prolactin Application Details Handling Images
Sequence MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGS-MNSQGSAQKA GTLLLLLISN LLFCQNVQPL PICSAGDCQT SLRELFDRVV ILSHYIHTLY TDMFIEFDKQ YVQDREFMVK VINDCPTSSL ATPEDKEQAL KVPPEVLLNL ILSLVQSSSD PLFQLITGVG GIQEAPEYIL SRAKEIEEQN KQLLEGVEKI ISQAYPEAKG NGIYFVWSQL PSLQGVDEES KILSLRNTIR CLRRDSHKVD NFLKVLRCQI AHQNNC
Specificity It has been selected for its ability to recognize PRL in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen Recombinant PRL expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against PRL.
Isotype IgG

Target Details Prolactin

Product Details anti-Prolactin Antibody Application Details Handling Images back to top
Antigen
Research Area Hormones
Pathways JAK-STAT Signaling

Application Details

Product Details anti-Prolactin Antibody Target Details Prolactin Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant PRL (Met1~Cys226) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-Prolactin Antibody Target Details Prolactin Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-Prolactin Antibody Target Details Prolactin Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Prolactin antibody (PRL) (AA 1-226) (ABIN1078459) anti-Prolactin (PRL) (AA 1-226) antibody