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Human Prolactin ELISA Kit

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Antigen
Prolactin (PRL) ELISA Kits
  • AV290867
  • prl
  • PRL
  • Prl1a1
  • PRLB
  • PRLSD1
  • Prol
  • prolactin
  • RATPRLSD1
  • RNPROL
Epitope
AA 29-227
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
27
22
17
11
10
8
4
4
3
3
3
2
2
2
1
1
Methode Type
Sandwich ELISA
Detection Range
156-10000 pg/mL
Minimum Detection Limit
156 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN1672760
$ 399.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32693866 ABIN417351 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 1.56-100 ng/mL 1.56 ng/mL Log in to see 2
0.32693866 ABIN2010759 Sandwich ELISA 0.1-100 ng/mL 0.1 ng/mL Log in to see 1
0.32693866 ABIN1029637 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 1.563-100 ng/mL 1.563 ng/mL Log in to see
0.32693866 ABIN2708363 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 156-10000 pg/mL 156 pg/mL Log in to see
0.0012117105 ABIN365109 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Cell Lysate 40-2000 μIU/mL 40 μIU/mL Log in to see 1
0.0012117105 ABIN512290 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 2.5-50 U/mL 2.5 U/mL Log in to see
0.0012117105 ABIN456249 Serum, Plasma, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012117105 ABIN1116668 Sandwich ELISA Serum, Plasma, Biological Fluids 1.56-100 ng/mL 1.56 ng/mL Log in to see
0.0012117105 ABIN649081 Sandwich ELISA Log in to see
0.0012117105 ABIN1326880 Sandwich ELISA Blood, Serum Log in to see 1
0.0012117105 ABIN2042640 Sandwich ELISA 6-10000 pg/mL 6 pg/mL Log in to see
0.0012117105 ABIN2683238 Serum 5-200 ng/mL 5 ng/mL Log in to see
0.0012117105 ABIN2685285 Sandwich ELISA Plasma (heparin), Plasma (citrate), Cell Culture Supernatant, Serum 156-10000 pg/mL 156 pg/mL Log in to see
0.0012117105 ABIN2064402 Serum Log in to see
0.0012117105 ABIN2685284 Sandwich ELISA Serum 0-200 ng/mL Log in to see
0.0012117105 ABIN2685304 Plasma, Serum, Tissue Culture Medium 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.0012117105 ABIN2762392 Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Cell Lysate 40-2000 μIU/mL 40 μIU/mL Log in to see
0.0012117105 ABIN2116317 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.0012117105 ABIN2538213 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 1.56-100 ng/mL 1.56 ng/mL Log in to see
0.0012117105 ABIN577076 Sandwich ELISA Log in to see

Top referenced Prolactin ELISA Kit for ELISA

Similar Prolactin ELISA Kits

Application / Reactivity Human
ELISA 25 ELISA Kits

General

Antigen Prolactin (PRL) ELISA Kits
Epitope AA 29-227
Reactivity Human
Kits with alternative reactivity to:
(27), (22), (17), (11), (10), (8), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1)
Methode Type Sandwich ELISA
Detection Range 156-10000 pg/mL
Minimum Detection Limit 156 pg/mL
Application ELISA
Pubmed 3 references available
Supplier Log in to see

Product Details Prolactin ELISA Kit

Target details Application Details Handling References for Prolactin Kit (ABIN1672760) Images
Purpose For quantitative detection of human Prolactin in cell culture supernates, serum and plasma(heparin, citrate).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate)
Detection Method Colorimetric
Specificity Natural and recombinant human Prolactin
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- human Prolactin antibod - 1
  • Lyophilized recombinant human Prolactin standar - 10ng/tubex2
  • Biotinylated anti- human Prolactin antibod - 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: L29-C227

Target details

Product Details Prolactin ELISA Kit Application Details Handling References for Prolactin Kit (ABIN1672760) Images back to top
Antigen
Alternative Name PRL (PRL ELISA Kit Abstract)
Background Prolactin, also calle PRL, is a protein that in humans is encodes by the PRL gene. This gene is mapped to 6p22.3. This gene encodes the anterior pituitary hormone prolactin. It may play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Prolactin also acts in a cytokine-like manner and as an important regulator of the immune system. Prolactin has important cell cycle related functions as a growth-, differentiating- and anti-apoptotic factor. As a growth factor binding to cytokine like receptors it has also profound influence on hematopoiesis,angiogenesis and is involved in the regulation of blood clotting through several pathways.
Gene ID 5617
UniProt P01236
Pathways JAK-STAT Signaling

Application Details

Product Details Prolactin ELISA Kit Target details Handling References for Prolactin Kit (ABIN1672760) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human Prolactin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Prolactin has been precoated onto 96-well plates. Standards(E.coli, L29-C227) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Prolactin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Prolactin amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Prolactin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Prolactin standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 1491, Standard deviation: 85, CV(%): 5.7
  • Sample 2: n=16, Mean(pg/ml): 2734, Standard deviation: 134, CV(%): 4.9
  • Sample 3: n=16, Mean(pg/ml): 4962, Standard deviation: 248.1, CV(%): 5,
  • Sample 1: n=24, Mean(pg/ml): 1529, Standard deviation: 96.33, CV(%): 6.3
  • Sample 2: n=24, Mean(pg/ml): 2850, Standard deviation: 196.7, CV(%): 6.9
  • Sample 3: n=24, Mean(pg/ml): 5271, Standard deviation: 390, CV(%): 7.4
Restrictions For Research Use only

Handling

Product Details Prolactin ELISA Kit Target details Application Details References for Prolactin Kit (ABIN1672760) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for Prolactin Kit (ABIN1672760)

Product Details Prolactin ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Xia, Qi, Zou, Yao: "Influenza A(H1N1) vaccination during early pregnancy transiently promotes hippocampal neurogenesis and working memory. Involvement of Th1/Th2 balance." in: Brain research, Vol. 1592, pp. 34-43, 2015

Background publications

Ge, Fernández, Moses, Greenspan: "Bone morphogenetic protein 1 processes prolactin to a 17-kDa antiangiogenic factor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 24, pp. 10010-5, 2007

Matsuda, Imaoka, Vomachka, Gudelsky, Hou, Mistry, Bailey, Nieport, Walther, Bader, Horseman: "Serotonin regulates mammary gland development via an autocrine-paracrine loop." in: Developmental cell, Vol. 6, Issue 2, pp. 193-203, 2004

Images

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Supplier Images
ELISA image for Prolactin ELISA Kit (PRL) (ABIN1672760) Human Prolactin PicoKine ELISA Kit standard curve