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anti-Pig (Porcine) Prolactin Receptor antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Prolactin Receptor Antibody (supplied by: Log in to see )

Antigen
Prolactin Receptor (PRLR) Antibodies
  • AI987712
  • DKFZp469D061
  • hPRLrI
  • Pr-1
  • Pr-3
  • PRL-R
  • PRLR
  • prlr
  • Prlr-rs1
  • PRLRb
  • RATPRLR
  • tprlr
Reactivity
Horse (Equine), Human, Pig (Porcine), Rabbit, Rat (Rattus)
94
33
17
1
Host
Mouse
56
45
7
4
Clonality
Monoclonal
Conjugate
This Prolactin Receptor antibody is un-conjugated
3
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
64
31
29
20
19
17
12
11
3
3
2
2
2
1
1
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Available images

Catalog No. ABIN152720
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Similar anti-Prolactin Receptor Antibodies

Application / Reactivity Rat (Rattus) Rabbit
ELISA 9 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 3 Antibodies 1 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 1 Antibodies
Immunofluorescence (IF) 3 Antibodies 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 6 Antibodies 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies 1 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Neutralization (Neut) 1 Antibodies
Western Blotting (WB) 20 Antibodies

General

Antigen Prolactin Receptor (PRLR) Antibodies
Reactivity Horse (Equine), Human, Pig (Porcine), Rabbit, Rat (Rattus)
(94), (33), (17), (1)
Host Mouse
(56), (45), (7), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Prolactin Receptor antibody is un-conjugated
(3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(64), (31), (29), (20), (19), (17), (12), (11), (3), (3), (2), (2), (2), (1), (1)
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Product Details anti-Prolactin Receptor Antibody

Target Details Prolactin Receptor Application Details Handling Images
Cross-Reactivity (Details) Not yet tested in other species.
Purification IgG purified
Immunogen Purified rat liver PRL receptor.
Clone U5
Isotype IgG1

Target Details Prolactin Receptor

Product Details anti-Prolactin Receptor Antibody Application Details Handling Images back to top
Antigen
Alternative Name Prolactin R (PRLR Antibody Abstract)
Background Prolactin (PRL) is a hormone involved in a variety of important functions including iontransport and osmoregulation, stimulation of milk, protein synthesis as well as theregulation of numerous reproductive functions. PRL exerts its influence on different celltypes through a signal transduction pathway which begins with the binding of thehormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a memberof the cytokine receptor family, varies in size (short and long forms) with tissue sourceand species, from ~40 kDa to 100 kDa. The PRL receptor consists of at least threeseparate domains: an extracellular region with 5 cysteines which contains the prolactinbinding site, a single transmembrane domain and a cytoplasmic region, the length ofwhich appears to influence ligand binding and regulate cellular function. Alternate Names: Anti-CLONE SPM213 antibody, anti-hPRL receptor antibody, anti-hPRLrI antibody, anti-PRL R antibody, anti- PRLR antibody.
Gene Symbol: PRLR
Gene ID 5618, 24684
Pathways JAK-STAT Signaling

Application Details

Product Details anti-Prolactin Receptor Antibody Target Details Prolactin Receptor Handling Images back to top
Application Notes This antibody can be used in Immunoprecipitation, Immunofluorescence, and Western Blot. By Western blot, this antibody detects an ~40 kDa protein and an ~100 kDa protein representing both the short and long forms of the PRL receptor, respectively, in various tissues. Immunohistochemical staining of PRL receptor in rat Islets of Langerhans with NB 300-561 results in a staining pattern consistent with cytoplasmic vesicle staining. NB 300-561, after biotinylation, has been used to detect human lymphocyte PRL receptor by Flow Cytometry. NB 300-561 does not inhibit the interaction of prolactin with the receptor. Recommended Starting Dilutions*: ICC: 5 µg/ml WB: 1 µg/ml *Optimal dilutions should be determined by the end user.
Recommended dilutions: Flow Cytometry 1:10-1:1000, Immunocytochemistry/Immunofluorescence 5 µg/mL, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Frozen 1:10-1:2000, Immunohistochemistry-Paraffin 1:10-1:2000, Immunoprecipitation 1:10-1:500, Western Blot 1 µg/mL
Restrictions For Research Use only

Handling

Product Details anti-Prolactin Receptor Antibody Target Details Prolactin Receptor Application Details Images back to top
Format Lyophilized
Reconstitution Reconstitute in 0.1 mL PBS.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-Prolactin Receptor Antibody Target Details Prolactin Receptor Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-Prolactin Receptor antibody (PRLR) (ABIN152720) Figure 1 shows immunohistochemical staining of PRL receptor in rat Islets of Langerha...