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|Antigen||Prolactin Receptor (PRLR) Antibodies|
|Epitope||AA 1-229 Alternatives|
|Reactivity||Rat (Rattus) Alternatives|
|Conjugate||This Prolactin Receptor antibody is un-conjugated Alternatives|
|1 reference available|
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Product Details anti-Prolactin Receptor AntibodyTarget Details Prolactin Receptor Application Details Handling References for anti-Prolactin Receptor antibody (ABIN2001649) Images
|Characteristics||Produced in rabbits immunized with purified, recombinant Rat PRLR (rR PRLR, ABIN2009108 P05710-1, Met 1-Asp 229,. Total IgG was purified by Protein A affinity chromatography.|
|Purification||purified by Protein A affinity chromatography|
|Sterility||0.2 μm filtered|
|Immunogen||Recombinant Rat PRLR protein (ABIN2009108)|
Target Details Prolactin ReceptorProduct Details anti-Prolactin Receptor Antibody Application Details Handling References for anti-Prolactin Receptor antibody (ABIN2001649) Images back to top
|Alternative Name||PRLR (PRLR Antibody Abstract)|
|Background||Prolactin receptor (PRLR) is a single-pass transmembrane receptor belonging to the type â..., cytokine receptor superfamily, and contains two fibronectin type-â...c domains. All class 1 ligands activate their respective receptors by clustering mechanisms. Ligand binding results in the transmembrane PRLR dimerization, followed by phosphorylation and activation of the molecules invloved in the signaling pathways, such as Jak-STAT, Ras/Raf/MAPK. The PRLR contains no intrinsic tyrosine kinase cytoplasmic domain but associates with a cytoplasmic tyrosine kinase, JAK2. PRLR mainly serves as the receptor for the pituitary hormone prolactin (PRL), a secreted hormone that affects reproduction and homeostasis in vertebrates. PRLR can be regulated by an interplay of two different mechanisms, PRL or ovarian steroid hormones independently or in combination in a tissue-specific manner. The role of the hormone prolactin (PRL) in the pathogenesis of breast cancer is mediated by its cognate receptor (PRLR). Ubiquitin-dependent degradation of the PRLR that negatively regulates PRL signaling is triggered by PRL-mediated phosphorylation of PRLR on Ser349 followed by the recruitment of the beta-transducin repeats-containing protein (beta- TrCP) ubiquitin-protein isopeptide ligase. which altered PRLR stability may directly influence the pathogenesis of breast cancer.|
|Pathways||JAK-STAT Signaling, Response to Growth Hormone Stimulus|
Application DetailsProduct Details anti-Prolactin Receptor Antibody Target Details Prolactin Receptor Handling References for anti-Prolactin Receptor antibody (ABIN2001649) Images back to top
ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Rat PRLR. The detection limit for Rat PRLR is approximately 0.039 ng/well.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Prolactin Receptor Antibody Target Details Prolactin Receptor Application Details References for anti-Prolactin Receptor antibody (ABIN2001649) Images back to top
|Buffer||0.2 μm filtered solution in PBS with 5 % trehalose|
|Handling Advice||Avoid repeated freeze-thaw cycles.|
|Storage||4 °C,-20 °C,-80 °C|
This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
|Expiry Date||12 months|
References for anti-Prolactin Receptor antibody (ABIN2001649)Product Details anti-Prolactin Receptor Antibody Target Details Prolactin Receptor Application Details Handling Images back to top
Goffin, Binart, Clément-Lacroix, Bouchard, Bole-Feysot, Edery, Lucas, Touraine, Pezet, Maaskant, Pichard, Helloco, Baran, Favre, Bernichtein, Allamando, Ormandy, Kelly: "From the molecular biology of prolactin and its receptor to the lessons learned from knockout mice models." in: Genetic analysis : biomolecular engineering, Vol. 15, Issue 3-5, pp. 189-201, 2000