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|Application / Reactivity||Rat (Rattus)||Human|
|ELISA||10 Antibodies||67 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||2 Antibodies|
|Flow Cytometry (FACS)||15 Antibodies|
|Functional Studies (Func)||1 Antibodies|
|Immunochromatography (IC)||2 Antibodies|
|Immunocytochemistry (ICC)||9 Antibodies|
|Immunofluorescence (IF)||7 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||13 Antibodies|
|Immunohistochemistry (IHC)||21 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||13 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||34 Antibodies|
|Immunoprecipitation (IP)||6 Antibodies|
|Neutralization (Neut)||1 Antibodies|
|Western Blotting (WB)||48 Antibodies|
|Cytometry by Time of Flight (CyTOF)||2 Antibodies|
|Dot Blot (DB)||1 Antibodies|
|Electron Microscopy (EM)||1 Antibodies|
|Antigen||Prolactin Receptor (PRLR) Antibodies|
|Epitope||AA 591-605, C-Term Alternatives|
|Reactivity||Human, Rat (Rattus) Alternatives|
|Conjugate||This Prolactin Receptor antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-Prolactin Receptor AntibodyTarget Details Prolactin Receptor Application Details Handling Images
Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Rabbit IgG polyclonal antibody for Prolactin receptor(PRLR) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: prolactin receptor
Protein Name: Prolactin receptor
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of mouse Prolactin Receptor(591-605aa QLGRLDYLDPTCFMH), identical to the related rat sequence, and different from the related human sequence by three amino acids.|
Target Details Prolactin ReceptorProduct Details anti-Prolactin Receptor Antibody Application Details Handling Images back to top
|Alternative Name||PRLR (PRLR Antibody Abstract)|
|Background||PRLR(Prolactin Receptor), is a cytokine receptor. By somatic cell hybrid analysis and by in situ hybridization, Arden et al.(1989, 1990) demonstrated that the prolactin receptor gene resides in the same chromosomal region as the growth hormone receptor gene, which has been mapped to 5p13-p12. Cunningham et al.(1990) demonstrated that zinc greatly increases the affinity of GH for the extracellular binding domain of PRLR, although it is not required for binding of GH to the growth hormone receptor or for binding of prolactin to the prolactin receptor. By mutational analysis, they showed that a cluster of 3 residues(histidine-18, histidine-21, and glutamic acid-174) in GH and histidine-188 in PRLR(conserved in all PRL receptors but not GH receptors) are likely zinc-ion ligands.|
|Pathways||JAK-STAT Signaling, Response to Growth Hormone Stimulus|
Application DetailsProduct Details anti-Prolactin Receptor Antibody Target Details Prolactin Receptor Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
HandlingProduct Details anti-Prolactin Receptor Antibody Target Details Prolactin Receptor Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
ImagesProduct Details anti-Prolactin Receptor Antibody Target Details Prolactin Receptor Application Details Handling back to top