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|Application / Reactivity||Human|
|Enzyme Immunoassay (EIA)||6 Antibodies|
|Flow Cytometry (FACS)||2 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||9 Antibodies|
|Immunofluorescence (IF)||29 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||4 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||29 Antibodies|
|Immunohistochemistry (IHC)||56 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||5 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||31 Antibodies|
|Immunoprecipitation (IP)||9 Antibodies|
|Western Blotting (WB)||138 Antibodies|
|Antigen||Protein-tyrosine Phosphatase 1C (PTPN6) Antibodies|
|Conjugate||This PTPN6 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-PTPN6 AntibodyTarget Details PTPN6 Application Details Handling References for anti-PTPN6 Antibody (ABIN264440) Images
|Specificity||Recognizes a protein of ~50 kDa, which is identified as the SH2-containing protein-tyrosine phosphatase 1 (SHP-1). This antibody is highly specific to SHP-1 and shows no cross-reaction with other protein-tyrosine phosphatase.|
|Characteristics||Synonyms: PTPN-6, HCP, PTP1C, EC=18.104.22.168, Protein-tyrosine phosphatase 1C, PTP-1C, SH-PTP1,Protein-tyrosine phosphatase SHP-1, Hematopoietic cell protein-tyrosine phosphatase,Tyrosine-protein phosphatase non-receptor type 6|
|Immunogen||Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized withrecombinant human SHP1 protein and mouse myeloma NS1 cells. Genename: PTPN6|
Target Details PTPN6Product Details anti-PTPN6 Antibody Application Details Handling References for anti-PTPN6 Antibody (ABIN264440) Images back to top
|Alternative Name||PTPN6 (PTPN6 Antibody Abstract)|
|Background||SHP-1 is known to play a crucial role in the regulation of hematopoiesis and it regulates the transcriptional activity stimulated by the erythropoietin (EPO)-induced JAK/STAT and MAPK pathways and is involved in the signaling machinery responsible for erythroid differentiation and suppression of apoptosis. Furthermore, the observation that SHP-1 associates with RAFTK/Pyk2/CAK activated by beta-chemokine receptor (CCR5) and SHP-1 also associates with the adaptor protein Grb2 and the Src-related kinase Syk indicates the role of SHP-1 in modulating cell migration, proliferation, and immune functions.Synonyms: HCP, Hematopoietic cell protein-tyrosine phosphatase, PTP-1C, PTP1C, PTPN-6, Protein-tyrosine phosphatase 1C, Protein-tyrosine phosphatase SHP-1, SH-PTP1, Tyrosine-protein phosphatase non-receptor type 6|
|Pathways||JAK-STAT Signaling, TLR Signaling, Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2|
Application DetailsProduct Details anti-PTPN6 Antibody Target Details PTPN6 Handling References for anti-PTPN6 Antibody (ABIN264440) Images back to top
Western Blot (1 μg/mL). Immunoprecipitation (2 g/mg of protein lysate). Positive Control: HeLa cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PTPN6 Antibody Target Details PTPN6 Application Details References for anti-PTPN6 Antibody (ABIN264440) Images back to top
|Buffer||PBS containing 0.08 % Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
|Expiry Date||12 months|
References for anti-PTPN6 Antibody (ABIN264440)Product Details anti-PTPN6 Antibody Target Details PTPN6 Application Details Handling Images back to top
Bittorf, Seiler, Zhang, Jaster, Brock: "SHP1 protein tyrosine phosphatase negatively modulates erythroid differentiation and suppression of apoptosis in J2E erythroleukemic cells." in: Biological chemistry, Vol. 380, Issue 10, pp. 1201-9, 2000
Ganju, Brubaker, Chernock, Avraham, Groopman: "Beta-chemokine receptor CCR5 signals through SHP1, SHP2, and Syk." in: The Journal of biological chemistry, Vol. 275, Issue 23, pp. 17263-8, 2000
Xie, Zhang, Siraganian: "Positive regulation of c-Jun N-terminal kinase and TNF-alpha production but not histamine release by SHP-1 in RBL-2H3 mast cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 3, pp. 1521-8, 2000
Daws, Eriksson, Oberg, Ullén, Sentman: "H-2Dd engagement of Ly49A leads directly to Ly49A phosphorylation and recruitment of SHP1." in: Immunology, Vol. 97, Issue 4, pp. 656-64, 1999
Bone, Dechert, Jirik, Schrader, Welham: "SHP1 and SHP2 protein-tyrosine phosphatases associate with betac after interleukin-3-induced receptor tyrosine phosphorylation. Identification of potential binding sites and substrates." in: The Journal of biological chemistry, Vol. 272, Issue 22, pp. 14470-6, 1997