We are preparing the requested document. Please wait, this may take a while...!
|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|Dot Blot (DB)||1 Antibodies||1 Antibodies|
|ELISA||12 Antibodies||20 Antibodies|
|Enzyme Immunoassay (EIA)||3 Antibodies||2 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies||2 Antibodies|
|Immunoelectron Microscopy (IEM)||1 Antibodies||1 Antibodies|
|Immunofluorescence (IF)||5 Antibodies||3 Antibodies|
|Immunohistochemistry (IHC)||10 Antibodies||6 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||7 Antibodies||5 Antibodies|
|Immunoprecipitation (IP)||3 Antibodies||3 Antibodies|
|Western Blotting (WB)||33 Antibodies|
|Antigen||Serotonin Receptor 1A (HTR1A) Antibodies|
|Epitope||AA 251-266, Intracellular Alternatives|
|Reactivity||Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Serotonin Receptor 1A antibody is un-conjugated Alternatives|
Immunohistochemistry (IHC), Western Blotting (WB)
|1 reference available|
|Supplier||Log in to see|
Product Details anti-Serotonin Receptor 1A AntibodyTarget Details Serotonin Receptor 1A Application Details Handling References for anti-Serotonin Receptor 1A Antibody (ABIN1742001) Images
|Cross-Reactivity (Details)||Mouse - identical. Will not recognize human 5-Hydroxytryptamine Receptor 1A.|
Confirmed by amino acid analysis and mass-spectrography.
Buffer after reconstitution:
Phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.05 % NaN3.
Antibody concentration after reconstitution:
1 µg peptide per 1 µg antibody.
|Purification||Affinity purified on immobilized antigen.|
|Immunogen||Peptide corresponding to amino acid residues 251-266 of rat 5-Hydroxytryptamine Receptor 1A. 3rd intracellular loop.|
Target Details Serotonin Receptor 1AProduct Details anti-Serotonin Receptor 1A Antibody Application Details Handling References for anti-Serotonin Receptor 1A Antibody (ABIN1742001) Images back to top
|Alternative Name||5-Hydroxytryptamine Receptor 1A (HTR1A Antibody Abstract)|
5-HT1A, Serotonin Receptor 1A
Serotonin [5-hydroxytryptamine (5-HT1)] is an important neurotransmitter in the CNS that exerts its neuronal activity via 5-HT receptors. 5-HT receptors are subdivided into seven families termed 5-HT1 through 5-HT7.1 The 5-HT receptor families belong to the G-Protein Coupled receptor superfamily with the exception of 5-HT3 receptor class which belongs to the ligand-gated ion channels. The 5-HT1 receptor class comprises of five different receptors (5-HT1A, 5-HT1B, 5-HT1D, 5-HT1e AND 5-HT1F) which share 41-63 % overall sequence identity and couple preferentially to Gi/Go to inhibit cAMP formation. The 5-HT1A receptor is the most widespread of all the 5-HT receptors. In the central nervous system, 5-HT1A receptors exist in the cerebral cortex, hippocampus, septum, amygdala and raphe nucelus in high densities, and was also demonstrated in a variety of tissues from various species. Activation of 5-HT1A receptor decreases blood pressure and heart rate, involved in learning, memory, relieving anxiety and depression. It has been designed to recognize 5-Hydroxytryptamine Receptor 1A from rat and mouse samples.
Application DetailsProduct Details anti-Serotonin Receptor 1A Antibody Target Details Serotonin Receptor 1A Handling References for anti-Serotonin Receptor 1A Antibody (ABIN1742001) Images back to top
WB: approx. 1:200,
IHC: approx. 1:100
|Restrictions||For Research Use only|
HandlingProduct Details anti-Serotonin Receptor 1A Antibody Target Details Serotonin Receptor 1A Application Details References for anti-Serotonin Receptor 1A Antibody (ABIN1742001) Images back to top
|Reconstitution||0.2 mL deionized water.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
Avoid multiple freezing and thawing.
Centrifuge all antibody preparations before use (10000 x g 5 min).
|Storage||4 °C/-20 °C|
|Storage Comment||The reconstituted solution can be stored at 4 °C for up to 2 weeks. For longer periods, small aliquots should be stored at -20 °C or below. The further dilutions should be made using a carrier protein such as BSA (1 %).|
References for anti-Serotonin Receptor 1A Antibody (ABIN1742001)Product Details anti-Serotonin Receptor 1A Antibody Target Details Serotonin Receptor 1A Application Details Handling Images back to top
Fink, Göthert: "5-HT receptor regulation of neurotransmitter release." in: Pharmacological reviews, Vol. 59, Issue 4, pp. 360-417, 2007 (PubMed).
ImagesProduct Details anti-Serotonin Receptor 1A Antibody Target Details Serotonin Receptor 1A Application Details Handling References for anti-Serotonin Receptor 1A Antibody (ABIN1742001) back to top