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|Antigen||Suppressor of Cytokine Signaling 6 (SOCS6) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This SOCS6 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Product Details anti-SOCS6 AntibodyTarget Details SOCS6 Application Details Handling Images
|Specificity||This antibody recognizes SOCS-6.|
Species reactivity (expected):Mouse.
Species reactivity (tested):Human.
|Immunogen||Synthetic peptide corresponding to C-terminal of human SOCS-6 protein.|
Target Details SOCS6Product Details anti-SOCS6 Antibody Application Details Handling Images back to top
|Alternative Name||SOCS6 / CIS4 (SOCS6 Antibody Abstract)|
|Background||The eight members of the recently identified Suppressor of Cytokines Signaling (SOCS) family are SOCS1, SOCS2, SOCS3, SOCS4, SOCS5, SOCS6, SOCS7, and CIS. Structurally the SOCS proteins are composed of an N- terminal region of variable length and amino acid composition, a central SH2 domain, and a C-terminal motif called the SOCS box. The SOCS proteins appear to form part of a classical negative feedback loop that regulates cytokine signal transduction. Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways. SOCS1 and SOCS6 interact with the insulin receptor (IR) when expressed in human hepatoma cells (HepG2) or in rat hepatoma cells overexpressing human IR. SOCS1 and SOCS6 inhibit insulin-dependent activation of ERK1/2 and protein kinase B in vivo and IR- directed phosphorylation of IRS1 in vitro. These results suggest that SOCS proteins may be inhibitors of IR signalling and could mediate cytokine-induced insulin resistance and contribute to the pathogenesis of type II diabetes.Synonyms: CIS-4, Cytokine-inducible SH2 protein 4, SOCS-4, SOCS-6, SOCS4, Suppressor of cytokine signaling 4, Suppressor of cytokine signaling 6|
|Molecular Weight||60-67 kDa|
|Gene ID||9306, 9606|
|Pathways||JAK-STAT Signaling, Carbohydrate Homeostasis|
Application DetailsProduct Details anti-SOCS6 Antibody Target Details SOCS6 Handling Images back to top
Western Blot: 1-2 μg/mL. Immunohistochemistry on paraffin sections: 1/100 for 30 min at RT, Staining offormalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Positive Control: A431 and HeLa cells. Placenta
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-SOCS6 Antibody Target Details SOCS6 Application Details Images back to top
|Buffer||PBS, pH 7.6 with 1 % BSA and 0.09 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!|