We are preparing the requested document. Please wait, this may take a while...!
|Application / Reactivity||Human||Chicken||Frog||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||315 Antibodies||14 Antibodies||2 Antibodies||138 Antibodies||108 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Intracellular Staining (ICS)||3 Antibodies||6 Antibodies||6 Antibodies||3 Antibodies|
|Immunoprecipitation (IP)||4 Antibodies||20 Antibodies||7 Antibodies||7 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||27 Antibodies||10 Antibodies||8 Antibodies||1 Antibodies|
|Immunohistochemistry (IHC)||150 Antibodies||6 Antibodies|
|Antigen||V-Src Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog (Avian) (SRC) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus), Chicken, Frog Alternatives|
|Conjugate||This SRC antibody is un-conjugated Alternatives|
Western Blotting (WB)
|10 references available|
|Supplier||Log in to see|
Product Details anti-SRC AntibodyTarget Details SRC Application Details Handling References for anti-SRC Antibody (ABIN307102) Images
|Specificity||Frog Fyn and Yes (92% homologous) were not tested for crossreactivity.|
Target Details SRCProduct Details anti-SRC Antibody Application Details Handling References for anti-SRC Antibody (ABIN307102) Images back to top
|Alternative Name||Src (SRC Antibody Abstract)|
|Background||Src (also known as pp60src) is a 60 kDa non-receptor tyrosine kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. Tyrosine 529 is located near the carboxyl terminus of Src and acts as a negative regulator, in that Src is held in the inactive form through an intramolecular interaction between the SH2 domain and the carboxyl terminus when tyrosine 529 is phosphorylated by Csk. This conformation blocks phosphorylation of the catalytic domain residue (tyrosine 418 in the human sequence), thereby preventing Src activation. When tyrosine 529 is dephosphorylated, tyrosine 418 can be maximally phosphorylated leading to full activation. Synonyms: pp60c-src, p60-Src, c-Src.|
|Pathways||JAK-STAT Signaling, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-SRC Antibody Target Details SRC Handling References for anti-SRC Antibody (ABIN307102) Images back to top
|Application Notes||Antibody can be used for Western blotting (1:1000 dilution). Can also be used for immunohistochemistry, however optimal dilution not determined. Positive Control: Chick Embryo Fibroblasts (CEF) cells expressing human wild-type Src protein, MDCK cells.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-SRC Antibody Target Details SRC Application Details References for anti-SRC Antibody (ABIN307102) Images back to top
References for anti-SRC Antibody (ABIN307102)Product Details anti-SRC Antibody Target Details SRC Application Details Handling Images back to top
Melendez, Turner, Avraham et al.: "Cardiomyocyte apoptosis triggered by RAFTK/pyk2 via Src kinase is antagonized by paxillin." in: The Journal of biological chemistry, Vol. 279, Issue 51, pp. 53516-23, 2004 (PubMed).
Pankov, Cukierman, Clark et al.: "Specific beta1 integrin site selectively regulates Akt/protein kinase B signaling via local activation of protein phosphatase 2A." in: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 18671-81, 2003 (PubMed).
Gil-Henn, Elson: "Tyrosine phosphatase-epsilon activates Src and supports the transformed phenotype of Neu-induced mammary tumor cells." in: The Journal of biological chemistry, Vol. 278, Issue 18, pp. 15579-86, 2003 (PubMed).
Bock, Herz: "Reelin activates SRC family tyrosine kinases in neurons." in: Current biology : CB, Vol. 13, Issue 1, pp. 18-26, 2003 (PubMed).
Lacalle, Mira, Gomez-Mouton et al.: "Specific SHP-2 partitioning in raft domains triggers integrin-mediated signaling via Rho activation." in: The Journal of cell biology, Vol. 157, Issue 2, pp. 277-89, 2002 (PubMed).
Obergfell, Eto, Mocsai et al.: "Coordinate interactions of Csk, Src, and Syk kinases with [alpha]IIb[beta]3 initiate integrin signaling to the cytoskeleton." in: The Journal of cell biology, Vol. 157, Issue 2, pp. 265-75, 2002 (PubMed).
Manzerra, Behrens, Canzoniero et al.: "Zinc induces a Src family kinase-mediated up-regulation of NMDA receptor activity and excitotoxicity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 20, pp. 11055-61, 2001 (PubMed).
Cheng, Bal, Kennedy et al.: "Attenuation of adhesion-dependent signaling and cell spreading in transformed fibroblasts lacking protein tyrosine phosphatase-1B." in: The Journal of biological chemistry, Vol. 276, Issue 28, pp. 25848-55, 2001 (PubMed).
Ma, Huang, Ali et al.: "Src tyrosine kinase is a novel direct effector of G proteins." in: Cell, Vol. 102, Issue 5, pp. 635-46, 2000 (PubMed).
Sussman, Welch, Walker et al.: "Altered focal adhesion regulation correlates with cardiomyopathy in mice expressing constitutively active rac1." in: The Journal of clinical investigation, Vol. 105, Issue 7, pp. 875-86, 2000 (PubMed).
ImagesProduct Details anti-SRC Antibody Target Details SRC Application Details Handling References for anti-SRC Antibody (ABIN307102) back to top