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anti-Frog SRC antibody for Western Blotting

Recommended SRC Antibody (supplied by: Log in to see )

Antigen
V-Src Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog (Avian) (SRC) Antibodies
  • AW259666
  • pp60c-src
  • C-src1
  • CG7524
  • D-Src64B
  • D-src
  • DSRC64
  • DSrc
  • DSrc64
  • DSrc64B
  • Dm SRC1
  • DmHD-358
  • Dmel\CG7524
  • Dsrc
  • Dsrc64
  • Dsrc64B
  • HD-358
  • MRE5
  • SRC 64B
  • Src
  • Src1
  • Src64
  • c-src
  • dSrc
  • dsrc
  • src
  • src1
  • src64
  • src64B
  • src64b
  • ASV
  • SRC1
  • c-SRC
  • p60-Src
  • c-Src
  • PP60C-SCR
  • SDR
  • v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
  • proto-oncogene tyrosine-protein kinase src
  • Rous sarcoma oncogene
  • Src oncogene at 64B
  • v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
  • v-src sarcoma viral oncogene
  • SRC
  • AaeL_AAEL002471
  • CpipJ_CPIJ010659
  • Src
  • Src64B
Epitope
pTyr529
59
31
31
27
25
24
18
15
15
11
11
8
8
6
5
5
5
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus), Chicken, Frog
408
238
193
29
17
16
13
11
9
7
6
5
5
4
4
4
1
1
1
1
1
1
1
Host
Rabbit
359
71
1
Conjugate
This SRC antibody is un-conjugated
19
15
15
14
13
13
3
3
2
2
2
2
2
2
2
2
2
2
Application
Western Blotting (WB)
358
199
143
49
37
27
24
20
12
9
5
5
3
2
1
Supplier
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Catalog No. ABIN307102
$ 618.20
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN229346 IHC WB Rabbit Log in to see Polyclonal

Top referenced anti-SRC antibody for Western Blotting

  • Human SRC Primary Antibody for WB - ABIN307102 (10 Publications): Melendez, Turner, Avraham, Steinberg, Schaefer, Sussman: Cardiomyocyte apoptosis triggered by RAFTK/pyk2 via Src kinase is antagonized by paxillin. in The Journal of biological chemistry 2004 (PubMed)

Similar anti-SRC Antibodies

Application / Reactivity Chicken Human Mouse (Murine) Rat (Rattus) Frog
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies 9 Antibodies 6 Antibodies 4 Antibodies
Western Blotting (WB) 338 Antibodies 24 Antibodies 2 Antibodies 197 Antibodies 158 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Intracellular Staining (ICS) 3 Antibodies 3 Antibodies 3 Antibodies 3 Antibodies
Immunoprecipitation (IP) 4 Antibodies 21 Antibodies 10 Antibodies 9 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 50 Antibodies 36 Antibodies

General

Antigen V-Src Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog (Avian) (SRC) Antibodies
Epitope pTyr529
(59), (31), (31), (27), (25), (24), (18), (15), (15), (11), (11), (8), (8), (6), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus), Chicken, Frog
(408), (238), (193), (29), (17), (16), (13), (11), (9), (7), (6), (5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(359), (71), (1)
Conjugate This SRC antibody is un-conjugated
(19), (15), (15), (14), (13), (13), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Western Blotting (WB)
(358), (199), (143), (49), (37), (27), (24), (20), (12), (9), (5), (5), (3), (2), (1)
Pubmed 10 references available
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Product Details anti-SRC Antibody

Target Details SRC Application Details Handling References for anti-SRC Antibody (ABIN307102) Images
Specificity Frog Fyn and Yes (92% homologous) were not tested for crossreactivity.
Clone Polyclonal

Target Details SRC

Product Details anti-SRC Antibody Application Details Handling References for anti-SRC Antibody (ABIN307102) Images back to top
Antigen
Alternative Name Src (SRC Antibody Abstract)
Background Src (also known as pp60src) is a 60 kDa non-receptor tyrosine kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. Tyrosine 529 is located near the carboxyl terminus of Src and acts as a negative regulator, in that Src is held in the inactive form through an intramolecular interaction between the SH2 domain and the carboxyl terminus when tyrosine 529 is phosphorylated by Csk. This conformation blocks phosphorylation of the catalytic domain residue (tyrosine 418 in the human sequence), thereby preventing Src activation. When tyrosine 529 is dephosphorylated, tyrosine 418 can be maximally phosphorylated leading to full activation. Synonyms: pp60c-src, p60-Src, c-Src.
UniProt P12931
Pathways JAK-STAT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-SRC Antibody Target Details SRC Handling References for anti-SRC Antibody (ABIN307102) Images back to top
Application Notes Antibody can be used for Western blotting (1:1000 dilution). Can also be used for immunohistochemistry, however optimal dilution not determined. Positive Control: Chick Embryo Fibroblasts (CEF) cells expressing human wild-type Src protein, MDCK cells.
Restrictions For Research Use only

Handling

Product Details anti-SRC Antibody Target Details SRC Application Details References for anti-SRC Antibody (ABIN307102) Images back to top
Format Liquid
Storage -80 °C

References for anti-SRC Antibody (ABIN307102)

Product Details anti-SRC Antibody Target Details SRC Application Details Handling Images back to top
Background publications

Melendez, Turner, Avraham, Steinberg, Schaefer, Sussman: "Cardiomyocyte apoptosis triggered by RAFTK/pyk2 via Src kinase is antagonized by paxillin." in: The Journal of biological chemistry, Vol. 279, Issue 51, pp. 53516-23, 2004

Bock, Herz: "Reelin activates SRC family tyrosine kinases in neurons." in: Current biology : CB, Vol. 13, Issue 1, pp. 18-26, 2003

Gil-Henn, Elson: "Tyrosine phosphatase-epsilon activates Src and supports the transformed phenotype of Neu-induced mammary tumor cells." in: The Journal of biological chemistry, Vol. 278, Issue 18, pp. 15579-86, 2003

Pankov, Cukierman, Clark, Matsumoto, Hahn, Poulin, Yamada: "Specific beta1 integrin site selectively regulates Akt/protein kinase B signaling via local activation of protein phosphatase 2A." in: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 18671-81, 2003

Lacalle, Mira, Gomez-Mouton, Jimenez-Baranda, Martinez-A, Manes: "Specific SHP-2 partitioning in raft domains triggers integrin-mediated signaling via Rho activation." in: The Journal of cell biology, Vol. 157, Issue 2, pp. 277-89, 2002

Obergfell, Eto, Mocsai, Buensuceso, Moores, Brugge, Lowell, Shattil: "Coordinate interactions of Csk, Src, and Syk kinases with [alpha]IIb[beta]3 initiate integrin signaling to the cytoskeleton." in: The Journal of cell biology, Vol. 157, Issue 2, pp. 265-75, 2002

Cheng, Bal, Kennedy, Tremblay: "Attenuation of adhesion-dependent signaling and cell spreading in transformed fibroblasts lacking protein tyrosine phosphatase-1B." in: The Journal of biological chemistry, Vol. 276, Issue 28, pp. 25848-55, 2001

Manzerra, Behrens, Canzoniero, Wang, Heidinger, Ichinose, Yu, Choi: "Zinc induces a Src family kinase-mediated up-regulation of NMDA receptor activity and excitotoxicity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 20, pp. 11055-61, 2001

Sussman, Welch, Walker, Klevitsky, Hewett, Price, Schaefer, Yager: "Altered focal adhesion regulation correlates with cardiomyopathy in mice expressing constitutively active rac1." in: The Journal of clinical investigation, Vol. 105, Issue 7, pp. 875-86, 2000

Ma, Huang, Ali, Lowry, Huang: "Src tyrosine kinase is a novel direct effector of G proteins." in: Cell, Vol. 102, Issue 5, pp. 635-46, 2000

Images

Product Details anti-SRC Antibody Target Details SRC Application Details Handling References for anti-SRC Antibody (ABIN307102) back to top
Supplier Images
Western Blotting (WB) image for anti-SRC antibody (V-Src Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog (Avian)) (pTyr529) (ABIN307102) Western blot analysis using Src [pY529] antibody on CEF cells untransfected (1) or tr...