You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human SRC antibody for Intracellular Staining

Recommended SRC Antibody (supplied by: Log in to see )

Antigen
V-Src Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog (Avian) (SRC) Antibodies
  • ASV
  • AW259666
  • c-src
  • c-SRC
  • c-Src
  • C-src1
  • CG7524
  • D-src
  • D-Src64B
  • Dmel\CG7524
  • DmHD-358
  • Dm SRC1
  • dsrc
  • DSrc
  • dSrc
  • Dsrc
  • DSRC64
  • DSrc64
  • Dsrc64
  • Dsrc64B
  • DSrc64B
  • HD-358
  • MRE5
  • p60-Src
  • PP60C-SCR
  • pp60c-src
  • SDR
  • src
  • Src
  • SRC1
  • src1
  • Src1
  • src64
  • Src64
  • SRC 64B
  • src64B
  • src64b
Epitope
pTyr418
76
34
29
27
25
22
19
15
14
13
11
8
6
6
5
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Human, Mouse (Murine), Rat (Rattus)
404
186
149
20
9
7
7
4
4
2
2
2
2
1
1
1
1
1
1
1
1
Host
Mouse
358
69
1
Clonality
Monoclonal
Conjugate
This SRC antibody is conjugated to Alexa Fluor 647
19
15
15
15
13
13
3
2
2
2
2
2
2
2
2
1
1
1
Application
Intracellular Staining (ICS)
349
200
158
37
33
24
23
20
14
7
5
5
2
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Catalog No. ABIN1177180
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008709465 ABIN1177181 ICS Alexa Fluor 488 Mouse IgG1, kappa pTyr418 Log in to see K98-37 3
0.0008709465 ABIN1177179 ICS PE Mouse IgG1, kappa pTyr418 Log in to see K98-37 3
0.0008709465 ABIN2680936 FACS ICS PE Mouse IgG2b kappa pTyr418 Log in to see SC1T2M3
0.0008709465 ABIN2680940 FACS ICS PerCP-eFluor® 710 Mouse IgG2b kappa pTyr418 Log in to see SC1T2M3
0.0008709465 ABIN2680942 FACS ICS EFluor 660 Mouse IgG2b kappa pTyr418 Log in to see SC1T2M3

Top referenced anti-SRC antibody for Intracellular Staining

Similar anti-SRC Antibodies

Application / Reactivity Human Chicken Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 328 Antibodies 14 Antibodies 147 Antibodies 117 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Intracellular Staining (ICS) 3 Antibodies 6 Antibodies 6 Antibodies 3 Antibodies
Immunoprecipitation (IP) 4 Antibodies 20 Antibodies 7 Antibodies 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 34 Antibodies 16 Antibodies 14 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 158 Antibodies 6 Antibodies 88 Antibodies

General

Antigen V-Src Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog (Avian) (SRC) Antibodies
Epitope pTyr418
(76), (34), (29), (27), (25), (22), (19), (15), (14), (13), (11), (8), (6), (6), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Human, Mouse (Murine), Rat (Rattus)
(404), (186), (149), (20), (9), (7), (7), (4), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(358), (69), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This SRC antibody is conjugated to Alexa Fluor 647
(19), (15), (15), (15), (13), (13), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Intracellular Staining (ICS)
(349), (200), (158), (37), (33), (24), (23), (20), (14), (7), (5), (5), (2), (1)
Pubmed 3 references available
Supplier Log in to see

Product Details anti-SRC Antibody

Target Details SRC Application Details Handling References for anti-SRC Antibody (ABIN1177180) Images
Brand BD Phosflow™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human Src Peptide
Clone K98-37
Isotype IgG1, kappa

Target Details SRC

Product Details anti-SRC Antibody Application Details Handling References for anti-SRC Antibody (ABIN1177180) Images back to top
Antigen
Alternative Name Src (SRC Antibody Abstract)
Background The non-receptor protein tyrosine kinases of the Src family have similar domain structures with considerable sequence homologies. They are associated with cell membranes and are activated by a wide variety of cell-surface receptors. Through their participation in cell-signaling cascades that mediate an array of cellular responses, they control essential cellular activities. Src, the prototype of the Src family, is encoded by the SRC protooncogene, which is a homolog of the v-Src gene of the Rous sarcoma virus. Src’s oncogenic potential may be a consequence of its role as a regulator of cell growth, morphology, motility, and adhesion. The protein contains domains that mediate protein-protein interactions and regulation of its tyrosine kinase activity. Mechanisms that regulate Src activity include phosphorylations of serines and tyrosines in most of the domains and interactions with receptor proteins, integrins, and other binding proteins. In particular, autophosphorylation at tyrosine 418 (Y418) activates tyrosine kinase activity. Phosphorylations at other sites are mediated by kinases such as cdc2, CSK, and CHK in signaling cascades and may up- or down-regulate Src activity. The K98-37 monoclonal antibody recognizes the phosphorylated Y418 in the protein kinase domain of activated Src. The orthologous phosphorylation sites in chicken, mouse, and rat Src are Y415, Y423, and Y418, respectively. Because of sequence similarities in this region, it may also recognize the homologous sites of some other Src family members: Lyn (pY396), Fyn (pY420), Hck (pY410), Lck (pY394), and Yes (pY425).
Synonyms: c-Src, p60-Src, SRC, SRC1
Research Area Signaling
Pathways JAK-STAT Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-SRC Antibody Target Details SRC Handling References for anti-SRC Antibody (ABIN1177180) Images back to top
Application Notes Either BD Cytofix™ fixation buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation. Any of the three BD™ Phosflow permeabilization buffers may be used.
Sample Volume 20 μL
Restrictions For Research Use only

Handling

Product Details anti-SRC Antibody Target Details SRC Application Details References for anti-SRC Antibody (ABIN1177180) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-SRC Antibody (ABIN1177180)

Product Details anti-SRC Antibody Target Details SRC Application Details Handling Images back to top
Product cited in:

Alvarez, Kantarjian, Cortes: "The role of Src in solid and hematologic malignancies: development of new-generation Src inhibitors." in: Cancer, Vol. 107, Issue 8, pp. 1918-29, 2006

Frame: "Newest findings on the oldest oncogene; how activated src does it." in: Journal of cell science, Vol. 117, Issue Pt 7, pp. 989-98, 2004

Arias-Salgado, Lizano, Sarkar, Brugge, Ginsberg, Shattil: "Src kinase activation by direct interaction with the integrin beta cytoplasmic domain." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 23, pp. 13298-302, 2003