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anti-Frog STAT1 antibody for Immunohistochemistry

Recommended STAT1 Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) Antibodies
  • 2010005J02Rik
  • AA408197
  • CANDF7
  • DD6G4-4
  • dZ182H3.3
  • H2RSP
  • IMUP
  • IMUP-1
  • IMUP-2
  • ISGF-3
  • si:by51f19.1
  • si:by134g18.2
  • si:dz73o4.2
  • si:dz182h3.3
  • si:dz199m19.1
  • STAT
  • STAT1
  • STAT4
  • STAT91
  • XStat1
Epitope
AA 1-194
94
78
70
27
23
20
18
18
14
12
11
11
7
6
5
5
4
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
556
241
178
28
17
16
15
13
7
5
3
3
2
2
2
2
2
2
1
1
Host
Mouse
370
184
20
6
3
3
1
Clonality
Monoclonal
Conjugate
This STAT1 antibody is un-conjugated
16
15
15
13
13
12
6
6
5
5
5
5
5
5
5
5
5
5
5
5
4
4
3
3
3
3
3
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
495
164
163
90
90
54
33
31
18
15
10
6
5
2
2
1
Supplier
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Catalog No. ABIN967758
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011340706 ABIN967759 IF IP IHC WB Mouse IgG1 AA 1-194 Log in to see 1-Stat1 5

Top referenced anti-STAT1 antibody for Immunohistochemistry

Similar anti-STAT1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Chicken Dog (Canine) Frog
Western Blotting (WB) 470 Antibodies 187 Antibodies 135 Antibodies 12 Antibodies 25 Antibodies 2 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Intracellular Staining (ICS) 18 Antibodies 14 Antibodies 2 Antibodies 2 Antibodies 2 Antibodies 4 Antibodies
Immunoprecipitation (IP) 94 Antibodies 45 Antibodies 34 Antibodies 8 Antibodies

General

Antigen Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) Antibodies
Epitope AA 1-194
(94), (78), (70), (27), (23), (20), (18), (18), (14), (12), (11), (11), (7), (6), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
(556), (241), (178), (28), (17), (16), (15), (13), (7), (5), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1)
Host Mouse
(370), (184), (20), (6), (3), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This STAT1 antibody is un-conjugated
(16), (15), (15), (13), (13), (12), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(495), (164), (163), (90), (90), (54), (33), (31), (18), (15), (10), (6), (5), (2), (2), (1)
Pubmed 5 references available
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Product Details anti-STAT1 Antibody

Target Details STAT1 Application Details Handling References for anti-STAT1 Antibody (ABIN967758) Images
Cross-Reactivity Chicken, Dog (Canine), Frog, Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human Stat1 aa. 1-194
Clone 1-Stat1
Isotype IgG1

Target Details STAT1

Product Details anti-STAT1 Antibody Application Details Handling References for anti-STAT1 Antibody (ABIN967758) Images back to top
Antigen
Alternative Name Stat1 (STAT1 Antibody Abstract)
Background Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat1 and Stat2 are components of the ISGF3 (Interferon-Stimulated Gene Factor 3) complex, which is the primary transcription activator induced by the binding of the interferon to a specific cell-surface receptor. Stat1 has two alternatively spliced isoforms, 91-kDa Stat1alpha and 84 kDa Stat1beta, Stat1alpha has 38 additional C-terminal amino acids. In response to the binding of IFNalpha, IFNgamma, EGF, PDGF, or CSF-1 to their respective receptors, the Stat1 subunits become tyrosine-phosphorylated at Y701, and the complex is translocated to the nucleus. This results in the formation of an active complex that includes the DNA-binding p48 subunit. This complex is responsible for modulating the transcription of the interferon-stimulated genes (ISGs). Thus, phosphorylation of Y701 in Stat1 occurs in response to growth factors and cytokines, and is essential for normal transcriptional activity of the ISGF3 complex. The 1/Stat1 monoclonal antibody recognizes the N-terminus of human Stat1 (both isoforms), regardless of phosphorylation status. This antibody is routinely tested by western blot analysis.
Molecular Weight 91/84 kDa
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-STAT1 Antibody Target Details STAT1 Handling References for anti-STAT1 Antibody (ABIN967758) Images back to top
Comment

Related Products: ABIN968533, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-STAT1 Antibody Target Details STAT1 Application Details References for anti-STAT1 Antibody (ABIN967758) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-STAT1 Antibody (ABIN967758)

Product Details anti-STAT1 Antibody Target Details STAT1 Application Details Handling Images back to top
Product cited in:

Doan, Ali, Bernstein: "Tyrosine kinase activation by the angiotensin II receptor in the absence of calcium signaling." in: The Journal of biological chemistry, Vol. 276, Issue 24, pp. 20954-8, 2001

Dupuis, Dargemont, Fieschi, Thomassin, Rosenzweig, Harris, Holland, Schreiber, Casanova: "Impairment of mycobacterial but not viral immunity by a germline human STAT1 mutation." in: Science (New York, N.Y.), Vol. 293, Issue 5528, pp. 300-3, 2001

Dumoutier, Louahed, Renauld: "Cloning and characterization of IL-10-related T cell-derived inducible factor (IL-TIF), a novel cytokine structurally related to IL-10 and inducible by IL-9." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 4, pp. 1814-9, 2000

Sadowski, Shuai, Darnell, Gilman: "A common nuclear signal transduction pathway activated by growth factor and cytokine receptors." in: Science (New York, N.Y.), Vol. 261, Issue 5129, pp. 1739-44, 1993

Fu, Schindler, Improta, Aebersold, Darnell: "The proteins of ISGF-3, the interferon alpha-induced transcriptional activator, define a gene family involved in signal transduction." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 16, pp. 7840-3, 1992

Images

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Supplier Images
Western Blotting (WB) image for anti-STAT1 antibody (Signal Transducer and Activator of Transcription 1, 91kDa) (AA 1-194) (ABIN967758) Western blot analysis of Stat1 on a A431 cell lysate. Lane 1: 1:250, lane 2: 1:500, l...
Immunofluorescence (IF) image for anti-STAT1 antibody (Signal Transducer and Activator of Transcription 1, 91kDa) (AA 1-194) (ABIN967758) Immunofluorescent staining of human fibroblast cells.