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anti-Human STAT1 antibody for Enzyme Immunoassay

Recommended STAT1 Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) Antibodies
  • 2010005J02Rik
  • AA408197
  • CANDF7
  • DD6G4-4
  • dZ182H3.3
  • H2RSP
  • IMUP
  • IMUP-1
  • IMUP-2
  • ISGF-3
  • si:by51f19.1
  • si:by134g18.2
  • si:dz73o4.2
  • si:dz182h3.3
  • si:dz199m19.1
  • STAT
  • STAT1
  • STAT4
  • STAT91
  • XStat1
Epitope
pSer727
86
75
69
18
18
14
12
11
9
7
6
6
5
5
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
474
214
173
26
16
15
15
14
7
5
4
2
2
2
2
2
2
1
1
Host
Rabbit
354
124
18
3
3
1
Clonality
Polyclonal
Conjugate
This STAT1 antibody is un-conjugated
13
12
12
12
8
8
5
5
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
417
155
143
89
61
48
33
24
18
9
9
6
5
2
2
1
Supplier
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Catalog No. ABIN358231
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000880999 ABIN500815 EIA IF IP WB Rabbit IgG C-Term Log in to see Polyclonal 4
0.000880999 ABIN401411 EIA WB Rabbit IgG C-Term Log in to see Polyclonal 1
0.000880999 ABIN784192 EIA IHC (p) WB Rabbit IgG pSer727 Log in to see Polyclonal
0.000880999 ABIN784212 EIA IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
0.000880999 ABIN318124 EIA IHC (p) IP WB Rabbit pTyr701 Log in to see Polyclonal
0.000880999 ABIN126895 EIA WB Mouse IgG1 phosphorylated Log in to see 12C5
0.000880999 ABIN1526107 EIA WB Mouse IgG1 Log in to see AT1F4
0.000880999 ABIN2732775 EIA WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-STAT1 antibody for Enzyme Immunoassay

Similar anti-STAT1 Antibodies

Application / Reactivity Human
BioImaging (BI) 2 Antibodies
Dot Blot (DB) 5 Antibodies
ELISA 127 Antibodies
Enzyme Immunoassay (EIA) 9 Antibodies
Flow Cytometry (FACS) 9 Antibodies
Immunochromatography (IC) 5 Antibodies
Immunocytochemistry (ICC) 23 Antibodies
Immunofluorescence (IF) 48 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies
Immunohistochemistry (IHC) 155 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 63 Antibodies
Immunoprecipitation (IP) 94 Antibodies
Intracellular Staining (ICS) 18 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies

General

Antigen Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) Antibodies
Epitope pSer727
(86), (75), (69), (18), (18), (14), (12), (11), (9), (7), (6), (6), (5), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(474), (214), (173), (26), (16), (15), (15), (14), (7), (5), (4), (2), (2), (2), (2), (2), (2), (1), (1)
Host Rabbit
(354), (124), (18), (3), (3), (1)
Clonality
Polyclonal
Conjugate This STAT1 antibody is un-conjugated
(13), (12), (12), (12), (8), (8), (5), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(417), (155), (143), (89), (61), (48), (33), (24), (18), (9), (9), (6), (5), (2), (2), (1)
Pubmed 5 references available
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Product Details anti-STAT1 Antibody

Target Details STAT1 Application Details Handling References for anti-STAT1 Antibody (ABIN358231) Images
Specificity This antibody detects STAT1 pSer727. Predicted to cross react with Mouse (100 % Antigen Homology).
Purification Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S727 of human STAT1.
Isotype Ig

Target Details STAT1

Product Details anti-STAT1 Antibody Application Details Handling References for anti-STAT1 Antibody (ABIN358231) Images back to top
Antigen
Alternative Name STAT1 (STAT1 Antibody Abstract)
Background In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT1 can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens.Synonyms: Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84
Molecular Weight 87335 Da
Gene ID 6772, 9606
UniProt P42224
Research Area Cancer, Transcription Factors, Signaling, Receptors, Inflammation
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-STAT1 Antibody Target Details STAT1 Handling References for anti-STAT1 Antibody (ABIN358231) Images back to top
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-STAT1 Antibody Target Details STAT1 Application Details References for anti-STAT1 Antibody (ABIN358231) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-STAT1 Antibody (ABIN358231)

Product Details anti-STAT1 Antibody Target Details STAT1 Application Details Handling Images back to top
Background publications

Garcin, Marq, Iseni, Martin, Kolakofsky: "A short peptide at the amino terminus of the Sendai virus C protein acts as an independent element that induces STAT1 instability." in: Journal of virology, Vol. 78, Issue 16, pp. 8799-811, 2004

Melén, Fagerlund, Nyqvist, Keskinen, Julkunen: "Expression of hepatitis C virus core protein inhibits interferon-induced nuclear import of STATs." in: Journal of medical virology, Vol. 73, Issue 4, pp. 536-47, 2004

Klampfer, Huang, Swaby, Augenlicht: "Requirement of histone deacetylase activity for signaling by STAT1." in: The Journal of biological chemistry, Vol. 279, Issue 29, pp. 30358-68, 2004

Marg, Shan, Meyer, Meissner, Brandenburg, Vinkemeier: "Nucleocytoplasmic shuttling by nucleoporins Nup153 and Nup214 and CRM1-dependent nuclear export control the subcellular distribution of latent Stat1." in: The Journal of cell biology, Vol. 165, Issue 6, pp. 823-33, 2004

Chim, Fung, Cheung, Liang, Kwong: "SOCS1 and SHP1 hypermethylation in multiple myeloma: implications for epigenetic activation of the Jak/STAT pathway." in: Blood, Vol. 103, Issue 12, pp. 4630-5, 2004

Images

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Supplier Images
 image for anti-STAT1 antibody (Signal Transducer and Activator of Transcription 1, 91kDa) (pSer727) (ABIN358231) anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pSer727) antibody
 image for anti-STAT1 antibody (Signal Transducer and Activator of Transcription 1, 91kDa) (pSer727) (ABIN358231) anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pSer727) antibody (Image 2)