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|Application / Reactivity||Human|
|BioImaging (BI)||2 Antibodies|
|Dot Blot (DB)||5 Antibodies|
|Enzyme Immunoassay (EIA)||9 Antibodies|
|Flow Cytometry (FACS)||9 Antibodies|
|Immunochromatography (IC)||5 Antibodies|
|Immunocytochemistry (ICC)||17 Antibodies|
|Immunofluorescence (IF)||47 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||33 Antibodies|
|Immunohistochemistry (IHC)||155 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||63 Antibodies|
|Immunoprecipitation (IP)||94 Antibodies|
|Intracellular Staining (ICS)||18 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Antigen||Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) Antibodies|
|Conjugate||This STAT1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-STAT1 AntibodyTarget Details STAT1 Application Details Handling References for anti-STAT1 Antibody (ABIN358231) Images
|Specificity||This antibody detects STAT1 pSer727. Predicted to cross react with Mouse (100 % Antigen Homology).|
|Purification||Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S727 of human STAT1.|
Target Details STAT1Product Details anti-STAT1 Antibody Application Details Handling References for anti-STAT1 Antibody (ABIN358231) Images back to top
|Alternative Name||STAT1 (STAT1 Antibody Abstract)|
|Background||In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT1 can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens.Synonyms: Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84|
|Molecular Weight||87335 Da|
|Gene ID||6772, 9606|
|Research Area||Cancer, Transcription Factors, Signaling, Receptors, Inflammation|
|Pathways||JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway|
Application DetailsProduct Details anti-STAT1 Antibody Target Details STAT1 Handling References for anti-STAT1 Antibody (ABIN358231) Images back to top
ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-STAT1 Antibody Target Details STAT1 Application Details References for anti-STAT1 Antibody (ABIN358231) Images back to top
|Buffer||PBS with 0.09 % (W/V) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-STAT1 Antibody (ABIN358231)Product Details anti-STAT1 Antibody Target Details STAT1 Application Details Handling Images back to top
Garcin, Marq, Iseni et al.: "A short peptide at the amino terminus of the Sendai virus C protein acts as an independent element that induces STAT1 instability." in: Journal of virology, Vol. 78, Issue 16, pp. 8799-811, 2004 (PubMed).
Melén, Fagerlund, Nyqvist et al.: "Expression of hepatitis C virus core protein inhibits interferon-induced nuclear import of STATs." in: Journal of medical virology, Vol. 73, Issue 4, pp. 536-47, 2004 (PubMed).
Marg, Shan, Meyer et al.: "Nucleocytoplasmic shuttling by nucleoporins Nup153 and Nup214 and CRM1-dependent nuclear export control the subcellular distribution of latent Stat1." in: The Journal of cell biology, Vol. 165, Issue 6, pp. 823-33, 2004 (PubMed).
Klampfer, Huang, Swaby et al.: "Requirement of histone deacetylase activity for signaling by STAT1." in: The Journal of biological chemistry, Vol. 279, Issue 29, pp. 30358-68, 2004 (PubMed).
Chim, Fung, Cheung et al.: "SOCS1 and SHP1 hypermethylation in multiple myeloma: implications for epigenetic activation of the Jak/STAT pathway." in: Blood, Vol. 103, Issue 12, pp. 4630-5, 2004 (PubMed).
ImagesProduct Details anti-STAT1 Antibody Target Details STAT1 Application Details Handling References for anti-STAT1 Antibody (ABIN358231) back to top