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anti-Human STAT1 antibody for Flow Cytometry

Recommended STAT1 Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) Antibodies
  • 2010005J02Rik
  • AA408197
  • CANDF7
  • DD6G4-4
  • dZ182H3.3
  • H2RSP
  • IMUP
  • IMUP-1
  • IMUP-2
  • ISGF-3
  • si:by51f19.1
  • si:by134g18.2
  • si:dz73o4.2
  • si:dz182h3.3
  • si:dz199m19.1
  • STAT
  • STAT1
  • STAT4
  • STAT91
  • XStat1
Epitope
pTyr701
96
78
68
27
23
20
18
18
15
14
12
11
7
6
5
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
560
244
180
29
17
16
16
14
7
5
4
3
2
2
2
2
2
2
1
1
Host
Mouse
368
187
20
9
3
3
1
Clonality
Monoclonal
Conjugate
This STAT1 antibody is conjugated to PE
16
15
14
13
13
13
6
6
6
5
5
5
5
5
5
5
5
5
5
5
4
4
3
3
3
3
3
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Intracellular Staining (ICS)
495
166
164
91
89
54
33
32
18
17
10
6
5
2
2
1
1
Supplier
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Catalog No. ABIN2680974
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.29105452 ABIN2680979 FACS ICS EFluor 450 Mouse IgG1 kappa pTyr701 Log in to see KIKSI0803
0.29105452 ABIN2680981 FACS ICS EFluor 660 Mouse IgG1 kappa pTyr701 Log in to see KIKSI0803
0.0010392143 ABIN218690 ICC FACS IF IP WB Rabbit IgG pTyr701 Log in to see Polyclonal
0.0010392143 ABIN222409 FACS Alexa Fluor 488 Rabbit IgG pTyr701 Log in to see
0.0010392143 ABIN4356296 FACS Alexa Fluor 700 Rat IgG2b AA 1-194 Log in to see 246523
0.0010392143 ABIN4356298 FACS Alexa Fluor 750 Rat IgG2b AA 1-194 Log in to see 246523
0.0010392143 ABIN4356297 FACS Alexa Fluor 647 Rat IgG2b AA 1-194 Log in to see 246523
0.0010392143 ABIN4356300 FACS Alexa Fluor 350 Rat IgG2b AA 1-194 Log in to see 246523
0.0010392143 ABIN4356301 FACS Alexa Fluor 405 Rat IgG2b AA 1-194 Log in to see 246523
0.0010392143 ABIN4356299 FACS Alexa Fluor 594 Rat IgG2b AA 1-194 Log in to see 246523
0.0010392143 ABIN4895283 FACS Allophycocyanin Rat IgG2b AA 1-194 Log in to see 246523
0.0010392143 ABIN4895284 FACS Alexa Fluor 488 Rat IgG2b AA 1-194 Log in to see 246523
0.0010392143 ABIN2680977 FACS ICS WB Mouse IgG1 kappa pTyr701 Log in to see KIKSI0803
0.0010392143 ABIN2367411 FACS IP WB Mouse IgG2b Log in to see SM1
0.0010392143 ABIN2367432 FACS Alexa Fluor 488 Rabbit IgG phosphorylated Log in to see 7H64
0.0010392143 ABIN4898979 FACS ICC Rat IgG2b AA 1-194 Log in to see 246523
0.0010392143 ABIN4900633 FACS SimWes WB Rabbit IgG pTyr701 Log in to see Polyclonal 3
0.0010392143 ABIN2367403 IC FACS IF IP ELISA WB Rabbit IgG pTyr701 Log in to see Polyclonal

Top referenced anti-STAT1 antibody for Flow Cytometry

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General

Antigen Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) Antibodies
Epitope pTyr701
(96), (78), (68), (27), (23), (20), (18), (18), (15), (14), (12), (11), (7), (6), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(560), (244), (180), (29), (17), (16), (16), (14), (7), (5), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1)
Host Mouse
(368), (187), (20), (9), (3), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This STAT1 antibody is conjugated to PE
(16), (15), (14), (13), (13), (13), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Intracellular Staining (ICS)
(495), (166), (164), (91), (89), (54), (33), (32), (18), (17), (10), (6), (5), (2), (2), (1), (1)
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Product Details anti-STAT1 Antibody

Target Details STAT1 Application Details Handling Images
Specificity This KIKSI0803 monoclonal antibody recognizes signal transducer and activator of transcription 1 (STAT1) when phosphorylated on tyrosine 701. STAT proteins are activated by ligand binding to receptors, such as cytokine receptors, that associate with Janus kinase (JAK) family members. Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. Phosphorylation of STAT1 on tyrosine 701 by JAK1 and JAK2 is essential for STAT1 dimer formation, nuclear translocation, and DNA binding activity. In response to IFN gamma stimulation, STAT1 homodimerizes or forms heterodimers with STAT3 that can bind to GAS (IFN gamma-activated sequence) promoter elements. In response to either IFN alpha or IFN beta stimulation, STAT1 forms a heterodimer with STAT2 that can bind ISRE (IFN-stimulated response element) promoter elements.

Specificity of this KIKSI0803 clone was determined by ELISA, flow cytometry, and western blotting.
Characteristics This KIKSI0803 monoclonal antibody recognizes signal transducer and activator of transcription 1 (STAT1) when phosphorylated on tyrosine 701. STAT proteins are activated by ligand binding to receptors, such as cytokine receptors, that associate with Janus kinase (JAK) family members. Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. Phosphorylation of STAT1 on tyrosine 701 by JAK1 and JAK2 is essential for STAT1 dimer formation, nuclear translocation, and DNA binding activity. In response to IFN gamma stimulation, STAT1 homodimerizes or forms heterodimers with STAT3 that can bind to GAS (IFN gamma-activated sequence) promoter elements. In response to either IFN alpha or IFN beta stimulation, STAT1 forms a heterodimer with STAT2 that can bind ISRE (IFN-stimulated response element) promoter elements. Specificity of this KIKSI0803 clone was determined by ELISA, flow cytometry, and western blotting.
Synonyms: Synonyms: signal transducer and activator of transcription 1, pSTAT1
Clone KIKSI0803
Isotype IgG1 kappa

Target Details STAT1

Product Details anti-STAT1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name STAT1 (STAT1 Antibody Abstract)
Research Area Cancer, Transcription Factors, Signaling, Receptors, Inflammation
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-STAT1 Antibody Target Details STAT1 Handling Images back to top
Application Notes Applications reported: This KIKSI0803 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications tested: This KIKSI0803 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10 5 to 10 8 cells/test. Staining Protocol: We recommend using Protocol C: Two-step protocol: Fixation/Methanol. Protocol A: Two- step protocol: intracellular (cytoplasmic) proteins and Protocol B: One-step protocol: intracellular (nuclear) proteins cannot be used. All Protocols can be found in the Flow Cytometry Protocols: "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the Best Protocols Section under the Resources tab online.
Restrictions For Research Use only

Handling

Product Details anti-STAT1 Antibody Target Details STAT1 Application Details Images back to top
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Handling Advice Do not freeze. Light-sensitive material.
Storage 4 °C
Storage Comment Store at 2-8°C.

Images

Product Details anti-STAT1 Antibody Target Details STAT1 Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-STAT1 antibody (Signal Transducer and Activator of Transcription 1, 91kDa) (pTyr701) (PE) (ABIN2680974) anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pTyr701) antibody (PE)