You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human STAT1 antibody for Intracellular Staining

Recommended STAT1 Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) Antibodies
  • 2010005J02Rik
  • AA408197
  • CANDF7
  • DD6G4-4
  • dZ182H3.3
  • H2RSP
  • IMUP
  • IMUP-1
  • IMUP-2
  • ISGF-3
  • si:by51f19.1
  • si:by134g18.2
  • si:dz73o4.2
  • si:dz182h3.3
  • si:dz199m19.1
  • STAT
  • STAT1
  • STAT4
  • STAT91
  • XStat1
Epitope
pSer727
75
72
69
18
18
14
12
11
8
7
6
6
5
5
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
440
213
173
26
16
15
15
14
7
5
4
2
2
2
2
2
2
1
1
Host
Mouse
355
107
3
3
1
Clonality
Monoclonal
Conjugate
This STAT1 antibody is conjugated to PE
11
10
9
8
7
7
5
5
3
3
3
3
3
3
3
1
1
1
1
1
1
Application
Intracellular Staining (ICS)
384
155
142
89
62
48
33
24
17
10
9
6
5
2
2
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN1177186
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008676631 ABIN1177183 ICS PerCP-Cy5.5 Mouse IgG2a pTyr701 Log in to see 4a 1
0.0008676631 ABIN1177187 ICS Alexa Fluor 647 Mouse IgG1, kappa pSer727 Log in to see K51-856 2
0.0008676631 ABIN1177188 ICS Alexa Fluor 488 Mouse IgG1, kappa pSer727 Log in to see K51-856 2
0.0008676631 ABIN968750 IF ICS IP WB Mouse IgG1 pTyr701 Log in to see 14-P 2
0.0008676631 ABIN968751 IF ICS IP WB Mouse IgG1 pTyr701 Log in to see 14-P 2
0.0008676631 ABIN1177182 ICS Pacific Blue Mouse IgG1, kappa pTyr701 Log in to see 14-P 1
0.0008676631 ABIN1177191 ICS Alexa Fluor 488 Mouse IgG2a pTyr701 Log in to see 4a 1
0.0008676631 ABIN1176937 ICS PE Mouse IgG1 AA 1-194, N-Term Log in to see 1-Stat1 1
0.0008676631 ABIN1176938 ICS Alexa Fluor 647 Mouse IgG1 AA 1-194, N-Term Log in to see 1-Stat1 1
0.0008676631 ABIN1177184 ICS PE Mouse IgG2a pTyr701 Log in to see 4a 1
0.0008676631 ABIN1177190 ICS Alexa Fluor 647 Mouse IgG2a pTyr701 Log in to see 4a 6
0.0008676631 ABIN1177185 ICS Alexa Fluor 647 Mouse IgG2a pTyr701 Log in to see 4a 1
0.0008676631 ABIN1177189 ICS PE Mouse IgG2a pTyr701 Log in to see 4a
0.0008676631 ABIN2680977 FACS ICS WB Mouse IgG1 kappa pTyr701 Log in to see KIKSI0803
0.0008676631 ABIN2680974 FACS ICS PE Mouse IgG1 kappa pTyr701 Log in to see KIKSI0803
0.0008676631 ABIN2680979 FACS ICS EFluor 450 Mouse IgG1 kappa pTyr701 Log in to see KIKSI0803
0.0008676631 ABIN2680981 FACS ICS EFluor 660 Mouse IgG1 kappa pTyr701 Log in to see KIKSI0803

Top referenced anti-STAT1 antibody for Intracellular Staining

Similar anti-STAT1 Antibodies

Application / Reactivity Human Mouse (Murine)
Western Blotting (WB) 363 Antibodies 161 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Intracellular Staining (ICS) 18 Antibodies 14 Antibodies
Immunoprecipitation (IP) 94 Antibodies 45 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 63 Antibodies 34 Antibodies
Immunohistochemistry (IHC) 155 Antibodies 86 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies 33 Antibodies
Immunofluorescence (IF) 48 Antibodies

General

Antigen Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) Antibodies
Epitope pSer727
(75), (72), (69), (18), (18), (14), (12), (11), (8), (7), (6), (6), (5), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(440), (213), (173), (26), (16), (15), (15), (14), (7), (5), (4), (2), (2), (2), (2), (2), (2), (1), (1)
Host Mouse
(355), (107), (3), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This STAT1 antibody is conjugated to PE
(11), (10), (9), (8), (7), (7), (5), (5), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1)
Application Intracellular Staining (ICS)
(384), (155), (142), (89), (62), (48), (33), (24), (17), (10), (9), (6), (5), (2), (2), (1)
Pubmed 1 reference available
Supplier Log in to see

Product Details anti-STAT1 Antibody

Target Details STAT1 Application Details Handling References for anti-STAT1 Antibody (ABIN1177186) Images
Brand BD Phosflow™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human Stat1 Peptide
Clone K51-856
Isotype IgG1, kappa

Target Details STAT1

Product Details anti-STAT1 Antibody Application Details Handling References for anti-STAT1 Antibody (ABIN1177186) Images back to top
Antigen
Alternative Name Stat1 (STAT1 Antibody Abstract)
Background Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat1 and Stat2 are components of the ISGF3 (Interferon-Stimulated Gene Factor 3) complex, which is the primary transcription activator induced by the binding of the interferon to a specific cell-surface receptor. Stat1 has two alternatively spliced isoforms, 91-kDa Stat1alpha and 84-kDa Stat1beta, Stat1alpha has 38 additional C-terminal amino acids. In response to the binding of IFNalpha, IFNgamma, EGF, PDGF, or CSF-1 to their respective receptors, the Stat1 subunits become tyrosine-phosphorylated at Y701, and the complex is translocated to the nucleus. This results in the formation of an active complex that includes the DNA-binding p48 subunit. This complex is responsible for modulating the transcription of the interferon-stimulated genes (ISGs). Furthermore, phosphorylation of serine 727 (S727) of Stat1 may occur in response to pathogens, cytokines, UV irradiation, and engagement of T and B cell antigen receptors. This second phosphorylation event is necessary for fully effective activation of transcription and may differentially regulate gene activation by interacting with other regulatory factors, such as p53 in the apoptotic pathway. The K51-856 monoclonal antibody recognizes the phosphorylated S727 in the C-terminal transactivating domain of human Stat1alpha, this site is not present in Stat1beta.
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-STAT1 Antibody Target Details STAT1 Handling References for anti-STAT1 Antibody (ABIN1177186) Images back to top
Application Notes Either BD Cytofix™ fixation buffer or BD Phosflow™ Fix Buffer I may be used for cell fixation. Any of the three BD Phosflow™ permeabilization buffers may be used.
Sample Volume 20 μL
Restrictions For Research Use only

Handling

Product Details anti-STAT1 Antibody Target Details STAT1 Application Details References for anti-STAT1 Antibody (ABIN1177186) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

References for anti-STAT1 Antibody (ABIN1177186)

Product Details anti-STAT1 Antibody Target Details STAT1 Application Details Handling Images back to top
Product cited in:

Heim: "The Jak-STAT pathway: specific signal transduction from the cell membrane to the nucleus." in: European journal of clinical investigation, Vol. 26, Issue 1, pp. 1-12, 1996

Images

Product Details anti-STAT1 Antibody Target Details STAT1 Application Details Handling References for anti-STAT1 Antibody (ABIN1177186) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-STAT1 antibody (Signal Transducer and Activator of Transcription 1, 91kDa) (pSer727) (PE) (ABIN1177186) anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pSer727) antibody (PE)