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anti-Human STAT1 antibody for Intracellular Staining

Recommended STAT1 Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) Antibodies
  • IMUP
  • H2RSP
  • IMUP-1
  • IMUP-2
  • 2010005J02Rik
  • AA408197
  • DD6G4-4
  • CANDF7
  • ISGF-3
  • STAT91
  • STAT4
  • XStat1
  • STAT
  • STAT1
  • dZ182H3.3
  • si:by134g18.2
  • si:by51f19.1
  • si:dz182h3.3
  • si:dz199m19.1
  • si:dz73o4.2
  • signal transducer and activator of transcription 1, 91kDa
  • RIKEN cDNA 2200002D01 gene
  • signal transducer and activator of transcription 1
  • signal transducer and activator of transcription 4
  • STAT1
  • 2200002D01Rik
  • Stat1
  • stat1
  • stat4
Epitope
pTyr701
100
77
60
27
23
21
19
17
16
15
15
13
8
6
6
5
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
588
300
245
42
28
27
26
18
17
12
9
9
5
5
4
4
4
3
3
2
1
1
1
Host
Mouse
377
204
20
9
3
3
1
Clonality
Monoclonal
Conjugate
This STAT1 antibody is un-conjugated
17
16
14
14
14
14
6
6
6
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
2
2
1
1
Application
Immunofluorescence (IF), Intracellular Staining (ICS), Immunoprecipitation (IP), Western Blotting (WB)
517
182
164
101
97
52
52
30
15
13
10
5
5
4
2
1
1
Options
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Catalog No. ABIN968750
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN968751 IF ICS IP WB Mouse IgG1 pTyr701 Log in to see 14-P 2
1 ABIN1177186 ICS PE Mouse IgG1, kappa pSer727 Log in to see K51-856 1
1 ABIN1177183 ICS PerCP-Cy5.5 Mouse IgG2a pTyr701 Log in to see 4a 1
1 ABIN1177187 ICS Alexa Fluor 647 Mouse IgG1, kappa pSer727 Log in to see K51-856 2
1 ABIN1177188 ICS Alexa Fluor 488 Mouse IgG1, kappa pSer727 Log in to see K51-856 2
1 ABIN1177191 ICS Alexa Fluor 488 Mouse IgG2a pTyr701 Log in to see 4a 1
1 ABIN1176937 ICS PE Mouse IgG1 AA 1-194, N-Term Log in to see 1-Stat1 1
1 ABIN1176938 ICS Alexa Fluor 647 Mouse IgG1 AA 1-194, N-Term Log in to see 1-Stat1 1
1 ABIN1177182 ICS Pacific Blue Mouse IgG1, kappa pTyr701 Log in to see 14-P 1
1 ABIN1177190 ICS Alexa Fluor 647 Mouse IgG2a pTyr701 Log in to see 4a 6
1 ABIN1177184 ICS PE Mouse IgG2a pTyr701 Log in to see 4a 1
1 ABIN1177185 ICS Alexa Fluor 647 Mouse IgG2a pTyr701 Log in to see 4a 1
1 ABIN1177189 ICS PE Mouse IgG2a pTyr701 Log in to see 4a

Top referenced anti-STAT1 antibody for Intracellular Staining

Similar anti-STAT1 Antibodies

Application / Reactivity Mouse (Murine) Human
BioImaging (BI) 2 Antibodies 2 Antibodies
ELISA 62 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 3 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 15 Antibodies
Immunofluorescence (IF) 26 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 39 Antibodies
Immunohistochemistry (IHC) 116 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 76 Antibodies
Immunoprecipitation (IP) 62 Antibodies
Intracellular Staining (ICS) 14 Antibodies
Simple Western (SimWes) 1 Antibodies
Western Blotting (WB) 235 Antibodies
Dot Blot (DB) 5 Antibodies

General

Antigen Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) Antibodies
Epitope pTyr701
(100), (77), (60), (27), (23), (21), (19), (17), (16), (15), (15), (13), (8), (6), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(588), (300), (245), (42), (28), (27), (26), (18), (17), (12), (9), (9), (5), (5), (4), (4), (4), (3), (3), (2), (1), (1), (1)
Host Mouse
(377), (204), (20), (9), (3), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This STAT1 antibody is un-conjugated
(17), (16), (14), (14), (14), (14), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (2), (2), (1), (1)
Application Immunofluorescence (IF), Intracellular Staining (ICS), Immunoprecipitation (IP), Western Blotting (WB)
(517), (182), (164), (101), (97), (52), (52), (30), (15), (13), (10), (5), (5), (4), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-STAT1 Antibody

Target Details STAT1 Application Details Handling References for anti-STAT1 Antibody (ABIN968750) Images
Cross-Reactivity Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human Stat1 (pY701) Peptide
Clone 14-P
Isotype IgG1

Target Details STAT1

Product Details anti-STAT1 Antibody Application Details Handling References for anti-STAT1 Antibody (ABIN968750) Images back to top
Antigen
Alternative Name Stat1 (STAT1 Antibody Abstract)
Background Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat1 and Stat2 are components of the ISGF3 (Interferon-Stimulated Gene Factor 3) complex, which is the primary transcription activator induced by the binding of the interferon to a specific cell-surface receptor. Stat1 has two alternatively spliced isoforms, 91-kDa Stat1alpha and 84-kDa Stat1beta, Stat1alpha has 38 additional C-terminal amino acids. In response to the binding of IFNalpha, IFNgamma, EGF, PDGF, or CSF-1 to their respective receptors, the Stat1 subunits become tyrosine-phosphorylated at Y701, and the complex is translocated to the nucleus. This results in the formation of an active complex that includes the DNA-binding p48 subunit. This complex is responsible for modulating the transcription of the interferon-stimulated genes (ISGs).
The 14/P-STAT1monoclonal antibody recognizes the phosphorylated Y701 in Stat1alpha and Stat1beta.
Molecular Weight 91/84 kDa
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin

Application Details

Product Details anti-STAT1 Antibody Target Details STAT1 Handling References for anti-STAT1 Antibody (ABIN968750) Images back to top
Comment

Related Products: ABIN968533, ABIN968534, ABIN967389, ABIN967758, ABIN967804

Restrictions For Research Use only

Handling

Product Details anti-STAT1 Antibody Target Details STAT1 Application Details References for anti-STAT1 Antibody (ABIN968750) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-STAT1 Antibody (ABIN968750)

Product Details anti-STAT1 Antibody Target Details STAT1 Application Details Handling Images back to top
Background publications

Darnell: "STATs and gene regulation." in: Science (New York, N.Y.), Vol. 277, Issue 5332, pp. 1630-5, 1997

Product cited in:

Fu, Zhang: "Transcription factor p91 interacts with the epidermal growth factor receptor and mediates activation of the c-fos gene promoter." in: Cell, Vol. 74, Issue 6, pp. 1135-45, 1993

Images

Product Details anti-STAT1 Antibody Target Details STAT1 Application Details Handling References for anti-STAT1 Antibody (ABIN968750) back to top
Supplier Images
Western Blotting (WB) image for anti-STAT1 antibody (Signal Transducer and Activator of Transcription 1, 91kDa) (pTyr701) (ABIN968750) Western blot analysis for Stat1 (pY701) (far left figure). A431 cells (Human epitheli...
Immunofluorescence (IF) image for anti-STAT1 antibody (Signal Transducer and Activator of Transcription 1, 91kDa) (pTyr701) (ABIN968750) Immunofluorescence staining for Stat1 (pY701) (middle left figure). A431 cells (Human...
 image for anti-STAT1 antibody (Signal Transducer and Activator of Transcription 1, 91kDa) (pTyr701) (ABIN968750) anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pTyr701) antibody (Image 3)