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anti-Human STAT1 antibody for Immunofluorescence

Recommended STAT1 Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) Antibodies
  • 2010005J02Rik
  • AA408197
  • CANDF7
  • DD6G4-4
  • dZ182H3.3
  • H2RSP
  • IMUP
  • IMUP-1
  • IMUP-2
  • ISGF-3
  • si:by51f19.1
  • si:by134g18.2
  • si:dz73o4.2
  • si:dz182h3.3
  • si:dz199m19.1
  • STAT
  • STAT1
  • STAT4
  • STAT91
  • XStat1
Epitope
C-Term
87
75
68
18
18
14
12
11
9
7
6
6
5
5
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
474
213
172
26
16
15
15
14
7
5
4
2
2
2
2
2
2
1
1
Host
Rabbit
354
124
18
3
3
1
Clonality
Polyclonal
Conjugate
This STAT1 antibody is un-conjugated
13
12
12
12
8
8
5
5
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
417
155
143
88
62
47
33
24
18
9
9
6
5
2
2
1
Supplier
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Catalog No. ABIN500815
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3190414 ABIN968750 IF ICS IP WB Mouse IgG1 pTyr701 Log in to see 14-P 2
0.3190414 ABIN968751 IF ICS IP WB Mouse IgG1 pTyr701 Log in to see 14-P 2
0.3190414 ABIN968779 IF IP WB Mouse IgG2a pTyr701 Log in to see 4a 2
0.3190414 ABIN968780 IF IP WB Mouse IgG2a pTyr701 Log in to see 4a 2
0.3190414 ABIN967758 IF IP IHC WB Mouse IgG1 AA 1-194 Log in to see 1-Stat1 5
0.3190414 ABIN967759 IF IP IHC WB Mouse IgG1 AA 1-194 Log in to see 1-Stat1 5
0.3190414 ABIN967760 IF IP WB Rabbit Ig AA 1-194 Log in to see Polyclonal 5
0.3190414 ABIN967761 IF IP WB Rabbit Ig AA 1-194 Log in to see Polyclonal 5
0.3190414 ABIN2705254 IC IF IP IHC WB Rabbit C-Term, pSer727 Log in to see Polyclonal
0.3190414 ABIN1870637 IF IHC WB Rabbit IgG pSer727 Log in to see Polyclonal
0.3190414 ABIN563024 IF ELISA WB Mouse IgG2a kappa AA 613-712, partial Log in to see 1A8
0.3190414 ABIN520534 IF WB Mouse AA 1-712, full length Log in to see Polyclonal
0.3190414 ABIN3023640 IF IHC WB Rabbit pSer727 Log in to see Polyclonal
0.0010822722 ABIN1533008 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal 2
0.0010822722 ABIN196892 IF IHC (p) WB Rabbit pSer727 Log in to see Polyclonal 4
0.0010822722 ABIN362170 IF IHC WB Rabbit IgG pSer727 Log in to see Polyclonal 5
0.0010822722 ABIN1501168 IF IHC (p) WB Mouse IgG1 Log in to see 15H3
0.0010822722 ABIN2452627 IF IHC WB Mouse IgG1 Log in to see 4H9
0.0010822722 ABIN2452618 IF IHC WB Mouse IgG1 Log in to see 15H3
0.0010822722 ABIN1501169 IF IHC (p) WB Mouse IgG1 Log in to see 4H9

Top referenced anti-STAT1 antibody for Immunofluorescence

Similar anti-STAT1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
BioImaging (BI) 2 Antibodies 2 Antibodies
ELISA 38 Antibodies 52 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies 7 Antibodies
Immunochromatography (IC) 2 Antibodies 3 Antibodies
Immunocytochemistry (ICC) 8 Antibodies
Immunofluorescence (IF) 17 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies
Immunohistochemistry (IHC) 77 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 26 Antibodies
Immunoprecipitation (IP) 34 Antibodies
Intracellular Staining (ICS) 4 Antibodies
Western Blotting (WB) 130 Antibodies

General

Antigen Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) Antibodies
Epitope C-Term
(87), (75), (68), (18), (18), (14), (12), (11), (9), (7), (6), (6), (5), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(474), (213), (172), (26), (16), (15), (15), (14), (7), (5), (4), (2), (2), (2), (2), (2), (2), (1), (1)
Host Rabbit
(354), (124), (18), (3), (3), (1)
Clonality
Polyclonal
Conjugate This STAT1 antibody is un-conjugated
(13), (12), (12), (12), (8), (8), (5), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(417), (155), (143), (88), (62), (47), (33), (24), (18), (9), (9), (6), (5), (2), (2), (1)
Pubmed 4 references available
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Product Details anti-STAT1 Antibody

Target Details STAT1 Application Details Handling References for anti-STAT1 Antibody (ABIN500815) Images
Specificity This antibody detects STAT1 alpha (C-term) at a 91 kDa band. No reaction to the 84 kDa STAT1a.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification DEAE purified
Immunogen STAT1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human STAT1 alpha. Remarks: The sequences differ from the murine corresponding sequences by four amino acids.
Isotype IgG

Target Details STAT1

Product Details anti-STAT1 Antibody Application Details Handling References for anti-STAT1 Antibody (ABIN500815) Images back to top
Antigen
Alternative Name STAT1 (STAT1 Antibody Abstract)
Background STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6), Stat1, Stat3, Stat5alpha, and Stat5beta have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-alpha, IFN-beta, IFN-gamma and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). STAT1 has two forms, the 91 kDa STAT1alpha and the 84 kDa STAT1beta which are encoded by the same gene with splicing variant.Synonyms: Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84
Gene ID 6772
NCBI Accession NP_009330
Research Area Cancer, Transcription Factors, Signaling, Receptors, Inflammation
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-STAT1 Antibody Target Details STAT1 Handling References for anti-STAT1 Antibody (ABIN500815) Images back to top
Application Notes ELISA. Western blot: 1: 1000 - 1: 2000. Immunoprecipitation: 2 - 4 μg. Immunoflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-STAT1 Antibody Target Details STAT1 Application Details References for anti-STAT1 Antibody (ABIN500815) Images back to top
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

References for anti-STAT1 Antibody (ABIN500815)

Product Details anti-STAT1 Antibody Target Details STAT1 Application Details Handling Images back to top
Background publications

Leonard, OShea: "Jaks and STATs: biological implications." in: Annual review of immunology, Vol. 16, pp. 293-322, 1998

Darnell: "STATs and gene regulation." in: Science (New York, N.Y.), Vol. 277, Issue 5332, pp. 1630-5, 1997

Schindler, Darnell: "Transcriptional responses to polypeptide ligands: the JAK-STAT pathway." in: Annual review of biochemistry, Vol. 64, pp. 621-51, 1995

Schindler, Fu, Improta, Aebersold, Darnell: "Proteins of transcription factor ISGF-3: one gene encodes the 91-and 84-kDa ISGF-3 proteins that are activated by interferon alpha." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 16, pp. 7836-9, 1992

Images

Product Details anti-STAT1 Antibody Target Details STAT1 Application Details Handling References for anti-STAT1 Antibody (ABIN500815) back to top
Supplier Images
Immunofluorescence (IF) image for anti-STAT1 antibody (Signal Transducer and Activator of Transcription 1, 91kDa) (C-Term) (ABIN500815) Immunocytochemistry of STAT1 alpha in HeLa cells with this product at 10 μg/ml.
Western Blotting (WB) image for anti-STAT1 antibody (Signal Transducer and Activator of Transcription 1, 91kDa) (C-Term) (ABIN500815) Western blot analysis of STAT1 alpha in whole cell lysates from HeLa (H), Jurkat (J),...