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|Application / Reactivity||Human|
|BioImaging (BI)||2 Antibodies|
|Dot Blot (DB)||5 Antibodies|
|Enzyme Immunoassay (EIA)||9 Antibodies|
|Flow Cytometry (FACS)||15 Antibodies|
|Immunochromatography (IC)||5 Antibodies|
|Immunocytochemistry (ICC)||30 Antibodies|
|Immunofluorescence (IF)||55 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||33 Antibodies|
|Immunohistochemistry (IHC)||162 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||88 Antibodies|
|Immunoprecipitation (IP)||94 Antibodies|
|Intracellular Staining (ICS)||18 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Antigen||Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) Antibodies|
|Conjugate||This STAT1 antibody is un-conjugated Alternatives|
Immunoprecipitation (IP), Western Blotting (WB)
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Product Details anti-STAT1 AntibodyTarget Details STAT1 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of total STAT1.|
|Cross-Reactivity (Details)||Does not cross-react with related proteins.|
|Immunogen||Purified recombinant human STAT1 protein fragments expressed in E.coli|
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|Alternative Name||STAT1 (STAT1 Antibody Abstract)|
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors,STAT family members are phosphorylated by the receptor associated kinases,and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha,interferon-gamma,EGF,PDGF and IL6. This protein mediates the expression of a variety of genes,which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq,Jul 2008]
Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs),cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors,signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor,that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG),which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma),STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF),migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes,inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1,FGFR2,FGFR3 and FGFR4.
Subunit structure: Isoform alphahomodimerizes upon IFN-gamma induced phosphorylation. Heterodimer with STAT2 upon IFN-alpha/beta induced phosphorylation. The heterodimer STAT1:STAT2 forms the interferon-stimulated gene factor 3 complex (ISGF3) with IRF9. Interacts (phosphorylated at Ser 727) with PIAS1 (dimethylated on arginine),the interaction results in release of STAT1 from its target gene. Interacts with IFNAR1,the interaction requires the phosphorylation of IFNAR1 at 'Tyr-466'. Interacts with IFNAR2,NMI,PTK2/FAK1 and SRC. Interacts with ERBB4 (phosphorylated). Interacts with Sendai virus C', C, Y1 and Y2 proteins,Nipah virus P,V and W proteins,and rabies virus phosphoprotein preventing activation of ISRE and GAS promoter. Interacts with HCV core protein,the interaction results in STAT1 degradation.
Subcellular location: Cytoplasm. Nucleus. Note: Translocated into the nucleus upon tyrosine phosphorylation and dimerization,in response to IFN-gamma and signaling by activated FGFR1,FGFR2,FGFR3 or FGFR4.
Sequence similarities: Belongs to the transcription factor STAT family. Contains 1 SH2 domain.
|Molecular Weight||91 kDa|
|Research Area||Cancer, Transcription Factors, Signaling, Receptors, Inflammation|
|Pathways||JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway|
Application DetailsProduct Details anti-STAT1 Antibody Target Details STAT1 Handling Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-STAT1 Antibody Target Details STAT1 Application Details Images back to top
|Buffer||PBS (pH 7.4) containing with 0.02 % sodium azide, 0.1 mg/mL BSA and 50 % glycerol.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
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