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anti-Human STAT1 antibody for Immunoprecipitation

Recommended STAT1 Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) Antibodies
  • 2010005J02Rik
  • AA408197
  • CANDF7
  • DD6G4-4
  • dZ182H3.3
  • H2RSP
  • IMUP
  • IMUP-1
  • IMUP-2
  • ISGF-3
  • si:by51f19.1
  • si:by134g18.2
  • si:dz73o4.2
  • si:dz182h3.3
  • si:dz199m19.1
  • STAT
  • STAT1
  • STAT4
  • STAT91
  • XStat1
Reactivity
Human
553
240
178
29
17
16
15
14
7
5
4
3
2
2
2
2
2
2
1
1
Host
Mouse
369
182
20
6
3
3
1
Clonality
Monoclonal
Conjugate
This STAT1 antibody is un-conjugated
16
15
15
13
13
12
6
6
5
5
5
5
5
5
5
5
5
5
5
5
4
4
3
3
3
3
3
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
492
163
162
88
87
55
33
31
18
15
10
6
5
2
2
1
Supplier
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Catalog No. ABIN2169570
$ 392.86
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.51226807 ABIN950042 IP WB Rabbit AA 1-712, full length Log in to see Polyclonal
0.0008941957 ABIN500815 EIA IF IP WB Rabbit IgG C-Term Log in to see Polyclonal 4
0.0008941957 ABIN120730 IHC (p) IP WB Mouse IgG2b AA 721-733 Log in to see SM1 1
0.0008941957 ABIN120731 IP WB Mouse IgG1 pSer727 Log in to see PSM1 1
0.0008941957 ABIN967804 BI IP WB Mouse IgG2b AA 592-731 Log in to see 42-Stat1 5
0.0008941957 ABIN967805 BI IP WB Mouse IgG2b AA 592-731 Log in to see 42-Stat1 5
0.0008941957 ABIN968750 IF ICS IP WB Mouse IgG1 pTyr701 Log in to see 14-P 2
0.0008941957 ABIN968751 IF ICS IP WB Mouse IgG1 pTyr701 Log in to see 14-P 2
0.0008941957 ABIN968779 IF IP WB Mouse IgG2a pTyr701 Log in to see 4a 2
0.0008941957 ABIN968780 IF IP WB Mouse IgG2a pTyr701 Log in to see 4a 2
0.0008941957 ABIN967758 IF IP IHC WB Mouse IgG1 AA 1-194 Log in to see 1-Stat1 5
0.0008941957 ABIN967759 IF IP IHC WB Mouse IgG1 AA 1-194 Log in to see 1-Stat1 5
0.0008941957 ABIN94471 IHC (p) IP WB Mouse IgG2b AA 721-733 Log in to see SM1 5
0.0008941957 ABIN94473 IP WB Mouse IgG1 Ser727, pSer727 Log in to see PSM1 4
0.0008941957 ABIN967760 IF IP WB Rabbit Ig AA 1-194 Log in to see Polyclonal 5
0.0008941957 ABIN967761 IF IP WB Rabbit Ig AA 1-194 Log in to see Polyclonal 5
0.0008941957 ABIN271824 IHC (p) IP WB Rabbit Log in to see Polyclonal
0.0008941957 ABIN239822 IHC (p) IP WB Mouse IgG2b Log in to see SM1
0.0008941957 ABIN94469 IP WB Mouse IgG1 AA 8-23 Log in to see SM2 3
0.0008941957 ABIN2469995 IP IHC WB Mouse IgG2b AA 721-733 Log in to see SM1

Top referenced anti-STAT1 antibody for Immunoprecipitation

Similar anti-STAT1 Antibodies

Application / Reactivity Human
BioImaging (BI) 2 Antibodies
Dot Blot (DB) 5 Antibodies
ELISA 146 Antibodies
Enzyme Immunoassay (EIA) 9 Antibodies
Flow Cytometry (FACS) 15 Antibodies
Immunochromatography (IC) 5 Antibodies
Immunocytochemistry (ICC) 30 Antibodies
Immunofluorescence (IF) 55 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies
Immunohistochemistry (IHC) 162 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 88 Antibodies
Immunoprecipitation (IP) 94 Antibodies
Intracellular Staining (ICS) 18 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies

General

Antigen Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) Antibodies
Reactivity Human
(553), (240), (178), (29), (17), (16), (15), (14), (7), (5), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1)
Host Mouse
(369), (182), (20), (6), (3), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This STAT1 antibody is un-conjugated
(16), (15), (15), (13), (13), (12), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1)
Application Immunoprecipitation (IP), Western Blotting (WB)
(492), (163), (162), (88), (87), (55), (33), (31), (18), (15), (10), (6), (5), (2), (2), (1)
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Product Details anti-STAT1 Antibody

Target Details STAT1 Application Details Handling Images
Specificity This antibody detects endogenous levels of total STAT1.
Cross-Reactivity Monkey
Cross-Reactivity (Details) Does not cross-react with related proteins.
Purification Affinity purified
Immunogen Purified recombinant human STAT1 protein fragments expressed in E.coli
Clone 6C2-F7-D12
Isotype IgG2a

Target Details STAT1

Product Details anti-STAT1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name STAT1 (STAT1 Antibody Abstract)
Background The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors,STAT family members are phosphorylated by the receptor associated kinases,and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha,interferon-gamma,EGF,PDGF and IL6. This protein mediates the expression of a variety of genes,which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq,Jul 2008]
Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs),cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors,signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor,that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG),which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma),STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF),migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes,inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1,FGFR2,FGFR3 and FGFR4.
Subunit structure: Isoform alphahomodimerizes upon IFN-gamma induced phosphorylation. Heterodimer with STAT2 upon IFN-alpha/beta induced phosphorylation. The heterodimer STAT1:STAT2 forms the interferon-stimulated gene factor 3 complex (ISGF3) with IRF9. Interacts (phosphorylated at Ser 727) with PIAS1 (dimethylated on arginine),the interaction results in release of STAT1 from its target gene. Interacts with IFNAR1,the interaction requires the phosphorylation of IFNAR1 at 'Tyr-466'. Interacts with IFNAR2,NMI,PTK2/FAK1 and SRC. Interacts with ERBB4 (phosphorylated). Interacts with Sendai virus C', C, Y1 and Y2 proteins,Nipah virus P,V and W proteins,and rabies virus phosphoprotein preventing activation of ISRE and GAS promoter. Interacts with HCV core protein,the interaction results in STAT1 degradation.
Subcellular location: Cytoplasm. Nucleus. Note: Translocated into the nucleus upon tyrosine phosphorylation and dimerization,in response to IFN-gamma and signaling by activated FGFR1,FGFR2,FGFR3 or FGFR4.
Sequence similarities: Belongs to the transcription factor STAT family. Contains 1 SH2 domain.
Molecular Weight 91 kDa
Gene ID 6772
NCBI Accession NM_007315
UniProt P42224
Research Area Cancer, Transcription Factors, Signaling, Receptors, Inflammation
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-STAT1 Antibody Target Details STAT1 Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-STAT1 Antibody Target Details STAT1 Application Details Images back to top
Format Liquid
Buffer PBS (pH 7.4) containing with 0.02 % sodium azide, 0.1 mg/mL BSA and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Images

Product Details anti-STAT1 Antibody Target Details STAT1 Application Details Handling back to top
Supplier Images
Immunoprecipitation (IP) image for anti-STAT1 antibody (Signal Transducer and Activator of Transcription 1, 91kDa) (ABIN2169570) anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) antibody
Western Blotting (WB) image for anti-STAT1 antibody (Signal Transducer and Activator of Transcription 1, 91kDa) (ABIN2169570) anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) antibody (Image 2)