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|Antigen||Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This STAT1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|1 reference available|
|Supplier||Log in to see|
Product Details anti-STAT1 AntibodyTarget Details STAT1 Application Details Handling ProductDetails: References for anti-STAT1 antibody (ABIN401411) Images
|Specificity||This antibody is specific for Stat1 protein.|
Species reactivity (expected):Rat, Human.
Species reactivity (tested):Mouse.
|Purification||Affinity chromatography on Protein A|
|Immunogen||Synthetic peptide corresponding to residues near the carboxy terminus of mouse Stat1 protein|
Target Details STAT1Product Details anti-STAT1 Antibody Application Details Handling ProductDetails: References for anti-STAT1 antibody (ABIN401411) Images back to top
|Alternative Name||STAT1 (STAT1 Antibody Abstract)|
|Background||Signal transducer and activator of transcription 1 (Stat1) belongs to a family of cytoplasmic transcription factors that can be activated (phosphorylated) by a cell surface receptor. Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation and DNA binding. Stat1 has two isoforms, Stat1alpha (91 kDa) and the splice variant Stat1beta (84 kDa). Stat1 has been found to be inappropriately activated in many tumors. In addition to tyrosine phosphorylation, Stat1 is phosphorylated through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway at Ser727 in response to IFN-gamma and other cellular stresses. Stat1 is localized in the cytoplasm and upon phosphorylation at Y701 is translocated to the nucleus.Synonyms: Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84|
|Research Area||Cancer, Transcription Factors, Signaling, Receptors, Inflammation|
|Pathways||JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events|
Application DetailsProduct Details anti-STAT1 Antibody Target Details STAT1 Handling ProductDetails: References for anti-STAT1 antibody (ABIN401411) Images back to top
ELISA: 1/25,000. Western Blot: 1/500 - 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-STAT1 Antibody Target Details STAT1 Application Details ProductDetails: References for anti-STAT1 antibody (ABIN401411) Images back to top
|Concentration||2.3 mg/mL (by UV absorbance at 280 nm)|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
ProductDetails: References for anti-STAT1 antibody (ABIN401411)Product Details anti-STAT1 Antibody Target Details STAT1 Application Details Handling Images back to top
Yu, Jove: "The STATs of cancer--new molecular targets come of age." in: Nature reviews. Cancer, Vol. 4, Issue 2, pp. 97-105, 2004