anti-Cow (Bovine) STAT3 antibody for Intracellular Staining

Recommended STAT3 Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) Antibodies
  • 1110034C02Rik
  • AW109958
  • Aprf
  • APRF
  • HIES
  • Xstat3
  • aprf
  • hies
  • stat3
  • wu:fc15d02
  • wu:fl59g06
  • z-Stat3
  • signal transducer and activator of transcription 3 (acute-phase response factor)
  • signal transduction and activation of transcription 3
  • signal transducer and activator of transcription 3
  • signal transducer and activator of transcription 3 (acute-phase response factor), gene 1
  • STAT3
  • stat3
  • Stat3
  • stat3.1
Epitope
pTyr705
126
107
75
28
18
17
16
16
15
13
11
6
6
6
5
5
5
4
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chimpanzee, Cow (Bovine), Human, Mouse (Murine)
623
364
287
40
37
33
29
16
11
10
7
5
5
5
4
3
3
2
1
1
1
1
Host
Mouse
480
148
34
6
2
1
Clonality
Monoclonal
Conjugate
This STAT3 antibody is conjugated to Pacific Blue
28
24
20
14
12
11
10
7
5
5
5
3
3
2
2
2
2
2
2
2
2
2
1
1
1
Application
Intracellular Staining (ICS)
562
244
229
94
87
85
74
59
27
12
11
9
8
6
5
4
3
3
3
2
2
2
2
1
1
1
1
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Catalog No. ABIN1177198
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1177195 ICS PerCP-Cy5.5 Mouse IgG2a, kappa pTyr705 Log in to see 4-P 1

General

Antigen Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) Antibodies
Epitope pTyr705
(126), (107), (75), (28), (18), (17), (16), (16), (15), (13), (11), (6), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chimpanzee, Cow (Bovine), Human, Mouse (Murine)
(623), (364), (287), (40), (37), (33), (29), (16), (11), (10), (7), (5), (5), (5), (4), (3), (3), (2), (1), (1), (1), (1)
Host Mouse
(480), (148), (34), (6), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This STAT3 antibody is conjugated to Pacific Blue
(28), (24), (20), (14), (12), (11), (10), (7), (5), (5), (5), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Intracellular Staining (ICS)
(562), (244), (229), (94), (87), (85), (74), (59), (27), (12), (11), (9), (8), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-STAT3 Antibody

Target Details STAT3 Application Details Handling References for anti-STAT3 antibody (ABIN1177198) Images
Brand BD Phosflow™
Purification Purified from tissue culture supernatant or ascites by affinity chromatography
Immunogen Phosphorylated Human Stat3 Peptide
Clone 4-P
Isotype IgG2a, kappa

Target Details STAT3

Product Details anti-STAT3 Antibody Application Details Handling References for anti-STAT3 antibody (ABIN1177198) Images back to top
Antigen
Alternative Name Stat3 (STAT3 Antibody Abstract)
Background Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat3 is a 92-kDa protein that is activated as a DNA- binding protein through cytokines, such as IL-6, and growth factors, such as EGF. Stat3 activation occurs via tyrosine phosphorylation at Y705. Tyrosine phosphorylation in response to cytokine stimulation is generally mediated by JAK1. Upon activation, Stat3 dimerizes, translocates to the nucleus and binds DNA response elements, thereby regulating gene expression. It has been reported that Stat3 binds to DNA as a homodimer, but it is also capable of binding as a heterodimer with Stat1. In addition to tyrosine phosphorylation, Stat3 is also phosphorylated at S727 via the MAPK pathway. Stat3 is widely expressed and can bind to the sis-inducible element (SIE) site from the c-fos promoter. This site is similar to the GAS element that is present in IFN-gamma induced genes. Thus, phosphorylation of Y705 in Stat3 occurs in response to growth factors and cytokines, and is essential for normal transcription activity. The 4/P-STAT3 monoclonal antibody recognizes the phosphorylated Y705 of Stat3.
Synonyms: Acute-phase response factor, APRF
Research Area Inflammation
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-STAT3 Antibody Target Details STAT3 Handling References for anti-STAT3 antibody (ABIN1177198) Images back to top
Application Notes This antibody conjugate is suitable for intracellular staining of human whole blood (using BD™ Phosflow Lyse/Fix Buffer) and peripheral blood mononuclear cells and cell lines (using BD Cytofix™ Fixation Buffer).
Sample Volume 20 μL
Restrictions For Research Use only

Handling

Product Details anti-STAT3 Antibody Target Details STAT3 Application Details References for anti-STAT3 antibody (ABIN1177198) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody is conjugated to Pacific Blue™ under optimum conditions, and unreacted Pacific Blue™ was removed.

References for anti-STAT3 antibody (ABIN1177198)

Product Details anti-STAT3 Antibody Target Details STAT3 Application Details Handling Images back to top
Product cited in:

Kanai, Konda, Nakajima, Izumi, Kanda, Nanakin, Kubohara, Chiba: "Differentiation-inducing factor-1 (DIF-1) inhibits STAT3 activity involved in gastric cancer cell proliferation via MEK-ERK-dependent pathway." in: Oncogene, Vol. 22, Issue 4, pp. 548-54, 2003

Images

Product Details anti-STAT3 Antibody Target Details STAT3 Application Details Handling References for anti-STAT3 antibody (ABIN1177198) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (pTyr705) antibody (Pacific Blue) (ABIN1177198) anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (pTyr705) antibody (Pacific Blue)