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anti-Human STAT3 antibody for Intracellular Staining

Recommended STAT3 Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) Antibodies
  • 1110034C02Rik
  • APRF
  • Aprf
  • aprf
  • AW109958
  • hies
  • HIES
  • stat3
  • wu:fc15d02
  • wu:fl59g06
  • Xstat3
  • z-Stat3
Epitope
pTyr705
106
94
84
28
21
17
16
15
14
13
13
8
6
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
519
247
192
30
24
23
12
11
10
5
4
3
2
2
2
2
1
1
1
1
1
Host
Mouse
453
96
34
4
1
1
Clonality
Monoclonal
Conjugate
This STAT3 antibody is un-conjugated
24
22
18
13
12
11
7
4
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Intracellular Staining (ICS), Western Blotting (WB)
484
233
206
74
69
65
46
25
23
12
12
12
9
7
3
3
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN968833
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00087032805 ABIN968834 ICS WB Mouse IgG2a, kappa pTyr705 Log in to see 4-P 4
0.00087032805 ABIN1177198 ICS Pacific Blue Mouse IgG2a, kappa pTyr705 Log in to see 4-P 1
0.00087032805 ABIN1177194 ICS Alexa Fluor 488 Mouse IgG1 pSer727 Log in to see 49-p 3
0.00087032805 ABIN1177201 ICS Alexa Fluor 488 Mouse IgG2a, kappa pTyr705 Log in to see 4-P 1
0.00087032805 ABIN1177193 ICS Alexa Fluor 647 Mouse IgG1 pSer727 Log in to see 49-p 2
0.00087032805 ABIN1177200 ICS Alexa Fluor 647 Mouse IgG2a, kappa pTyr705 Log in to see 4-P 3
0.00087032805 ABIN1177195 ICS PerCP-Cy5.5 Mouse IgG2a, kappa pTyr705 Log in to see 4-P 1
0.00087032805 ABIN1177192 ICS PE Mouse IgG1 pSer727 Log in to see 49-p 1
0.00087032805 ABIN1177197 ICS Alexa Fluor 647 Mouse IgG2a, kappa pTyr705 Log in to see 4-P 1
0.00087032805 ABIN1177199 ICS PE Mouse IgG2a, kappa pTyr705 Log in to see 4-P
0.00087032805 ABIN1177196 ICS PE Mouse IgG2a, kappa pTyr705 Log in to see 4-P
0.00087032805 ABIN2680983 FACS ICS APC Mouse IgG2b kappa pTyr705 Log in to see LUVNKLA

Top referenced anti-STAT3 antibody for Intracellular Staining

Similar anti-STAT3 Antibodies

Application / Reactivity Mouse (Murine) Human
Chromatin Immunoprecipitation (ChIP) 1 Antibodies
ELISA 55 Antibodies
Enzyme Immunoassay (EIA) 8 Antibodies
Flow Cytometry (FACS) 11 Antibodies
Gel Shift (GS) 1 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 21 Antibodies
Immunofluorescence (IF) 48 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 107 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 31 Antibodies
Immunoprecipitation (IP) 49 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Intracellular Staining (ICS) 13 Antibodies
Western Blotting (WB) 190 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 5 Antibodies

General

Antigen Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) Antibodies
Epitope pTyr705
(106), (94), (84), (28), (21), (17), (16), (15), (14), (13), (13), (8), (6), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(519), (247), (192), (30), (24), (23), (12), (11), (10), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(453), (96), (34), (4), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This STAT3 antibody is un-conjugated
(24), (22), (18), (13), (12), (11), (7), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Intracellular Staining (ICS), Western Blotting (WB)
(484), (233), (206), (74), (69), (65), (46), (25), (23), (12), (12), (12), (9), (7), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-STAT3 Antibody

Target Details STAT3 Application Details Handling References for anti-STAT3 Antibody (ABIN968833) Images
Cross-Reactivity Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human Stat3 Peptide
Clone 4-P
Isotype IgG2a, kappa

Target Details STAT3

Product Details anti-STAT3 Antibody Application Details Handling References for anti-STAT3 Antibody (ABIN968833) Images back to top
Antigen
Alternative Name Stat3 (STAT3 Antibody Abstract)
Background Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat3 is a 92-kDa protein that is activated as a DNA- binding protein through cytokines, such as IL-6, and growth factors, such as EGF. Stat3 activation occurs via tyrosine phosphorylation at Y705. Tyrosine phosphorylation in response to cytokine stimulation is generally mediated by JAK1. Upon activation, Stat3 dimerizes, translocates to the nucleus and binds DNA response elements, thereby regulating gene expression. It has been reported that Stat3 binds to DNA as a homodimer, but it is also capable of binding as a heterodimer with Stat1. In addition to tyrosine phosphorylation, Stat3 is also phosphorylated at S727 via the MAPK pathway. Stat3 is widely expressed and can bind to the sis-inducible element (SIE) site from the c-fos promoter. This site is similar to the GAS element that is present in IFN-gamma induced genes. Thus, phosphorylation of Y705 in Stat3 occurs in response to growth factors and cytokines, and is essential for normal transcription activity.
The 4/P-STAT3 monoclonal antibody recognizes the phosphorylated Y705 of Stat3.
Molecular Weight 92 kDa
Research Area Signaling, Receptors, Inflammation
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-STAT3 Antibody Target Details STAT3 Handling References for anti-STAT3 Antibody (ABIN968833) Images back to top
Comment

Related Products: ABIN968533, ABIN968534, ABIN967389, ABIN967808

Restrictions For Research Use only

Handling

Product Details anti-STAT3 Antibody Target Details STAT3 Application Details References for anti-STAT3 Antibody (ABIN968833) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-STAT3 Antibody (ABIN968833)

Product Details anti-STAT3 Antibody Target Details STAT3 Application Details Handling Images back to top
Product cited in:

Kanai, Konda, Nakajima, Izumi, Kanda, Nanakin, Kubohara, Chiba: "Differentiation-inducing factor-1 (DIF-1) inhibits STAT3 activity involved in gastric cancer cell proliferation via MEK-ERK-dependent pathway." in: Oncogene, Vol. 22, Issue 4, pp. 548-54, 2003

Smith, Crompton: "Expression of v-src in mammary epithelial cells induces transcription via STAT3." in: The Biochemical journal, Vol. 331 ( Pt 2), pp. 381-5, 1998

Fu, Zhang: "Transcription factor p91 interacts with the epidermal growth factor receptor and mediates activation of the c-fos gene promoter." in: Cell, Vol. 74, Issue 6, pp. 1135-45, 1993

Background publications

Darnell: "STATs and gene regulation." in: Science (New York, N.Y.), Vol. 277, Issue 5332, pp. 1630-5, 1997

Images

Product Details anti-STAT3 Antibody Target Details STAT3 Application Details Handling References for anti-STAT3 Antibody (ABIN968833) back to top
Supplier Images
Western Blotting (WB) image for anti-STAT3 antibody (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor)) (pTyr705) (ABIN968833) Western blot analysis for Stat3 (pY705). A431 cells (Human epithelial carcinoma, ATCC...