anti-Human STAT3 antibody for Immunohistochemistry (Frozen Sections)

Recommended STAT3 Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) Antibodies
  • 1110034C02Rik
  • AW109958
  • Aprf
  • APRF
  • HIES
  • Xstat3
  • aprf
  • hies
  • stat3
  • wu:fc15d02
  • wu:fl59g06
  • z-Stat3
  • signal transducer and activator of transcription 3 (acute-phase response factor)
  • signal transduction and activation of transcription 3
  • signal transducer and activator of transcription 3
  • signal transducer and activator of transcription 3 (acute-phase response factor), gene 1
  • STAT3
  • stat3
  • Stat3
  • stat3.1
Epitope
pTyr705
124
107
76
18
17
17
16
16
15
13
11
6
6
5
5
5
5
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine)
606
351
275
40
37
32
29
16
11
10
7
5
5
5
4
3
3
2
1
1
1
1
1
Host
Mouse
477
135
34
5
2
1
Clonality
Monoclonal
Conjugate
This STAT3 antibody is un-conjugated
28
24
19
14
12
11
9
6
4
4
4
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
555
244
228
91
84
83
62
55
27
12
11
8
8
6
5
3
3
3
2
2
2
2
2
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN126898
$ 555.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN126899 EIA IHC (fro) WB Mouse IgG1 phosphorylated Log in to see 23G5
1 ABIN126896 EIA IHC (fro) IF IP WB Biotin Mouse IgG1 pTyr705 Log in to see 9-00E-012
1 ABIN2476595 IHC (fro) ELISA WB Goat IgG C-Term Log in to see Polyclonal 1
1 ABIN1174971 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 335-546 Log in to see Polyclonal
1 ABIN640506 IHC (fro) ELISA WB Goat C-Term Log in to see

General

Antigen Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) Antibodies
Epitope pTyr705
(124), (107), (76), (18), (17), (17), (16), (16), (15), (13), (11), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine)
(606), (351), (275), (40), (37), (32), (29), (16), (11), (10), (7), (5), (5), (5), (4), (3), (3), (2), (1), (1), (1), (1), (1)
Host Mouse
(477), (135), (34), (5), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This STAT3 antibody is un-conjugated
(28), (24), (19), (14), (12), (11), (9), (6), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(555), (244), (228), (91), (84), (83), (62), (55), (27), (12), (11), (8), (8), (6), (5), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Supplier Log in to see

Product Details anti-STAT3 Antibody

Target Details STAT3 Application Details Handling Images
Specificity The STAT proteins serve as both cytoplasmic signal transducers and nuclear activators of transcription. STAT3 is activated by tyrosine phosphorylation at residue Tyr 705 in cells treated with interleukin 6 or EGF. Activated STAT3 can bind to DNA either as homodimer or as heterodimer with STAT1. AM00148PU-N specifically recognizes STAT3 phosphorylated at Tyr 705 in human, mouse and dog. The antibody does not crossreact with the non-phosphorylated form of STAT3 nor with unrelated tyrosine-phosphorylated proteins. AM00148PU-N is particularly suitable for Western blot and ELISA applications.
Characteristics Synonyms: STAT-3, Acute-phase response factor, APRF, Signal transducer and activator oftranscription 3
Purification Size exclusion chromatography
Components incl. positive Control
Immunogen Synthetic phosphopeptide conjuagted to KLH
Clone 9-00E-012
Isotype IgG1

Target Details STAT3

Product Details anti-STAT3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name STAT3 (STAT3 Antibody Abstract)
Background The STAT proteins serve as both cytoplasmic signal transducers and nuclear activators of transcription. STATs are mediators involved in cytokine signalling. In response to a specific cytokine signal, STAT proteins are phosphorylated on conserved tyrosine residues. Phosphorylated STAT proteins dimerize via their SH2 domains and move to the nucleus. The STAT dimers bind to specific DNA elements resulting in transcriptional regulation of downstream target genes. STAT3 is activated by tyrosine phosphorylation at residue Tyr 705 in cells treated with interleukin 6 or EGF. Activated STAT3 can bind to DNA either as homodimer or as heterodimer with STAT1.Synonyms: APRF, Acute-phase response factor, STAT-3, Signal transducer and activator of transcription 3
Molecular Weight 92 kDa
Gene ID 6774
UniProt P40763
Research Area Embryogenesis, Embryonic stem cells, Transcription Factors, Signaling, Receptors, Inflammation
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-STAT3 Antibody Target Details STAT3 Handling Images back to top
Application Notes Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL. Immunoprecipitation: 1 - 10g per 106 pervanadate treated HepG2 cells. Immunocytochemistry: 1 - 10 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-STAT3 Antibody Target Details STAT3 Application Details Images back to top
Reconstitution Restore with 1 mL H2O (15 min, RT).
Buffer 1 mL2 x PBS with 0.09 % Sodium Azide, PEG and Sucrose
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C/-80 °C
Storage Comment Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-STAT3 Antibody Target Details STAT3 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (pTyr705) antibody (ABIN126898) anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (pTyr705) antibody