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anti-Mouse (Murine) STAT3 antibody for Flow Cytometry

Recommended STAT3 Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) Antibodies
  • 1110034C02Rik
  • APRF
  • Aprf
  • aprf
  • AW109958
  • hies
  • HIES
  • stat3
  • wu:fc15d02
  • wu:fl59g06
  • Xstat3
  • z-Stat3
Epitope
pTyr705
106
94
84
28
21
17
16
15
14
13
13
8
6
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
519
247
192
30
24
23
12
11
10
5
4
3
2
2
2
2
1
1
1
1
1
Host
Mouse
453
96
34
4
1
1
Clonality
Monoclonal
Conjugate
This STAT3 antibody is conjugated to APC
24
22
18
13
11
11
7
4
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Intracellular Staining (ICS)
485
233
206
74
69
65
46
24
23
12
12
12
9
7
3
3
2
2
2
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN2680983
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010666476 ABIN218705 FACS IP WB Mouse IgG1 pTyr705 Log in to see
0.0010666476 ABIN222411 FACS Alexa Fluor 647 Rabbit IgG pTyr705 Log in to see
0.0010666476 ABIN218702 ICC FACS IP ELISA WB Rabbit IgG pTyr705 Log in to see Polyclonal
0.0010666476 ABIN2179493 FACS PE Rabbit IgG AA 685-725, pTyr705 Log in to see Polyclonal
0.0010666476 ABIN2367681 FACS ELISA Alexa Fluor 647 Rabbit IgG pTyr705 Log in to see 7H65
0.0010666476 ABIN2367682 FACS IF IP IHC WB Rabbit IgG pTyr705 Log in to see 7H65
0.0010666476 ABIN2367680 FACS IP WB Mouse IgG1 pTyr705 Log in to see 3A27
0.0010666476 ABIN1982857 ICC FACS WB Sheep IgG AA 1-175 Log in to see Polyclonal
0.0010666476 ABIN2560183 FACS IF IHC WB Rabbit IgG Log in to see Polyclonal
0.0010666476 ABIN2969383 ICC FACS IF IHC WB Rabbit IgG Log in to see Polyclonal

Similar anti-STAT3 Antibodies

Application / Reactivity Mouse (Murine) Human
Chromatin Immunoprecipitation (ChIP) 1 Antibodies
ELISA 55 Antibodies
Enzyme Immunoassay (EIA) 8 Antibodies
Flow Cytometry (FACS) 11 Antibodies
Gel Shift (GS) 1 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 21 Antibodies
Immunofluorescence (IF) 48 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 107 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 31 Antibodies
Immunoprecipitation (IP) 49 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Intracellular Staining (ICS) 13 Antibodies
Western Blotting (WB) 190 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 5 Antibodies

General

Antigen Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) Antibodies
Epitope pTyr705
(106), (94), (84), (28), (21), (17), (16), (15), (14), (13), (13), (8), (6), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(519), (247), (192), (30), (24), (23), (12), (11), (10), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(453), (96), (34), (4), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This STAT3 antibody is conjugated to APC
(24), (22), (18), (13), (11), (11), (7), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Intracellular Staining (ICS)
(485), (233), (206), (74), (69), (65), (46), (24), (23), (12), (12), (12), (9), (7), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-STAT3 Antibody

Target Details STAT3 Application Details Handling Images
Specificity This LUVNKLA monoclonal antibody recognizes human and mouse signal transducer and activator of transcription 3 (STAT3) when phosphorylated on tyrosine 705 (Y705). The STAT family represents seven transcription factors (STATs 1, 2, 3, 4, 5A, 5B, and 6) that are involved in many cellular processes including apoptosis, cell differentiation, and proliferation in a cell type- and cytokine-specific manner. STAT proteins are activated by ligand binding to cytokine receptors that associate with Janus kinase (JAK) family members.

Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. STAT3 is activated downstream of numerous cytokines including interferons, IL-5, IL-6, IL-10, and LIF. STAT3 is important for the differentiation of Th17 cells and mediates a variety of cellular processes including cell growth and survival. The importance of STAT3 is highlighted by both loss-of-function and gain-of-function mutations. Deletion of STAT3 in T cells results in decreased IL-6- and IL-2-mediated proliferation, while deletion of STAT3 in neutrophils and macrophages results in increased susceptibility to LPS-induced endotoxic shock and increased production of the pro-inflammatory cytokines IL-6 and TNF alpha. Hyper STAT3 activity is associated with poor prognosis of many different cancers.
Characteristics This LUVNKLA monoclonal antibody recognizes human and mouse signal transducer and activator of transcription 3 (STAT3) when phosphorylated on tyrosine 705 (Y705). The STAT family represents seven transcription factors (STATs 1, 2, 3, 4, 5A, 5B, and 6) that are involved in many cellular processes including apoptosis, cell differentiation, and proliferation in a cell type- and cytokine-specific manner. STAT proteins are activated by ligand binding to cytokine receptors that associate with Janus kinase (JAK) family members. Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. STAT3 is activated downstream of numerous cytokines including interferons, IL-5, IL-6, IL-10, and LIF. STAT3 is important for the differentiation of Th17 cells and mediates a variety of cellular processes including cell growth and survival. The importance of STAT3 is highlighted by both loss-of-function and gain-of-function mutations. Deletion of STAT3 in T cells results in decreased IL-6- and IL-2-mediated proliferation, while deletion of STAT3 in neutrophils and macrophages results in increased susceptibility to LPS-induced endotoxic shock and increased production of the pro-inflammatory cytokines IL-6 and TNF alpha. Hyper STAT3 activity is associated with poor prognosis of many different cancers.
Synonyms: Synonyms: Signal transducer and activator of transcription 3
Clone LUVNKLA
Isotype IgG2b kappa

Target Details STAT3

Product Details anti-STAT3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name STAT3 (STAT3 Antibody Abstract)
Research Area Embryogenesis, Embryonic stem cells, Transcription Factors, Signaling, Receptors, Inflammation
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-STAT3 Antibody Target Details STAT3 Handling Images back to top
Application Notes Applications reported: This LUVNKLA antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications tested: This LUVNKLA antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10 5 to 10 8 cells/test. Staining Protocol: We recommend using Protocol C: Two-step protocol: Fixation/Methanol. Protocol A: Two- step protocol: intracellular (cytoplasmic) proteins and Protocol B: One-step protocol: intracellular (nuclear) proteins cannot be used. All Protocols can be found in the Flow Cytometry Protocols: "Staining Intracellular Page 2 of 2 Anti-Human/Mouse phospho-STAT3 (Y705) APC
Restrictions For Research Use only

Handling

Product Details anti-STAT3 Antibody Target Details STAT3 Application Details Images back to top
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Handling Advice Do not freeze. Light-sensitive material.
Storage 4 °C
Storage Comment Store at 2-8°C.

Images

Product Details anti-STAT3 Antibody Target Details STAT3 Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-STAT3 antibody (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor)) (pTyr705) (APC) (ABIN2680983) anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (pTyr705) antibody (APC)