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anti-Mouse (Murine) STAT3 antibody for Immunofluorescence

Recommended STAT3 Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) Antibodies
  • 1110034C02Rik
  • APRF
  • Aprf
  • aprf
  • AW109958
  • hies
  • HIES
  • stat3
  • wu:fc15d02
  • wu:fl59g06
  • Xstat3
  • z-Stat3
Epitope
pSer727
112
94
84
28
21
17
16
16
14
13
13
13
8
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
544
265
207
30
24
24
13
11
10
5
4
3
3
2
2
2
2
1
1
1
1
1
Host
Mouse
466
108
34
4
1
1
Clonality
Monoclonal
Conjugate
This STAT3 antibody is un-conjugated
24
22
18
13
12
11
8
5
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
502
238
216
79
75
70
52
32
23
13
12
12
9
7
3
3
2
2
2
2
1
1
1
1
Supplier
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Catalog No. ABIN968868
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.32106254 ABIN968869 IF WB Mouse IgG1 pSer727 Log in to see 49-p 4
0.32106254 ABIN1109152 EIA IF IHC (p) WB Mouse IgG1 Log in to see 3B5 2
0.32106254 ABIN2855865 ICC IF WB Rabbit IgG C-Term Log in to see Polyclonal
0.32106254 ABIN388367 IF WB Rabbit Ig pSer727 Log in to see Polyclonal
0.32106254 ABIN2705260 IC IF IP IHC WB Rabbit C-Term Log in to see Polyclonal
0.32106254 ABIN1870642 IF IHC WB Rabbit IgG pSer727 Log in to see Polyclonal
0.32106254 ABIN256912 ICC IF IHC (p) IP IHC WB Rabbit IgG Tyr705 Log in to see EP2147Y
0.32106254 ABIN2503761 IF IHC WB Rabbit Ig Fraction pSer727 Log in to see Polyclonal
0.32106254 ABIN2503759 IF Rabbit Ig Fraction pSer727 Log in to see Polyclonal
0.32106254 ABIN2424805 IF IHC WB Rabbit IgG pSer727 Log in to see Polyclonal
0.0010843406 ABIN2780479 IF IHC WB Rabbit N-Term Log in to see Polyclonal 2
0.0010843406 ABIN374087 EIA IF IHC (p) WB Goat C-Term, Isoform 1 Log in to see Polyclonal 1
0.0010843406 ABIN389664 IF IHC (p) WB Rabbit Ig pSer727 Log in to see Polyclonal 3
0.0010843406 ABIN967808 IF IP IHC WB Mouse IgG1 AA 1-175 Log in to see 84-Stat3 5
0.0010843406 ABIN967809 IF IP IHC WB Mouse IgG1 AA 1-175 Log in to see 84-Stat3 5
0.0010843406 ABIN196692 IF IHC (p) WB Rabbit pSer727 Log in to see Polyclonal 3
0.0010843406 ABIN126898 EIA IHC (fro) IF IP WB Mouse IgG1 pTyr705 Log in to see 9-00E-012
0.0010843406 ABIN126896 EIA IHC (fro) IF IP WB Biotin Mouse IgG1 pTyr705 Log in to see 9-00E-012
0.0010843406 ABIN871611 IF IHC WB Rabbit AA 703-707 Log in to see Polyclonal 3
0.0010843406 ABIN1109149 IF IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal

Top referenced anti-STAT3 antibody for Immunofluorescence

Similar anti-STAT3 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 454 Antibodies 201 Antibodies 157 Antibodies
Proximity Ligation Assay (PLA) 3 Antibodies
Intracellular Staining (ICS) 13 Antibodies 13 Antibodies 4 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies 2 Antibodies 2 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 54 Antibodies 50 Antibodies 30 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 71 Antibodies 37 Antibodies 33 Antibodies
Immunohistochemistry (IHC) 206 Antibodies 114 Antibodies 92 Antibodies

General

Antigen Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) Antibodies
Epitope pSer727
(112), (94), (84), (28), (21), (17), (16), (16), (14), (13), (13), (13), (8), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(544), (265), (207), (30), (24), (24), (13), (11), (10), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(466), (108), (34), (4), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This STAT3 antibody is un-conjugated
(24), (22), (18), (13), (12), (11), (8), (5), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Western Blotting (WB)
(502), (238), (216), (79), (75), (70), (52), (32), (23), (13), (12), (12), (9), (7), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-STAT3 Antibody

Target Details STAT3 Application Details Handling References for anti-STAT3 Antibody (ABIN968868) Images
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human Stat3
Clone 49-p
Isotype IgG1

Target Details STAT3

Product Details anti-STAT3 Antibody Application Details Handling References for anti-STAT3 Antibody (ABIN968868) Images back to top
Antigen
Alternative Name Stat3 (STAT3 Antibody Abstract)
Background The Stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Seven mammalian Stat proteins have been identified: Stat1-4, Stat5a, 5b, and Stat6. Stat3 is a 92-kDa protein that is activated as a DNA binding protein through cytokines, such as IL-6, and growth factors, such as EGF. Stat3 is phophorylated at serine 727 (S727) via the MAPK pathway. The S727 residue is located at a conserved Pro-X-Ser-Pro sequence, which is recognized by the protein kinase ERK. Activation through the S727 residue is thought to lead to initiation of transcription. Upon activation, Stat3 dimerizes, translocates to the nucleus, and binds DNA response elements thereby regulating gene expression. It appears that Stat3 binds to DNA as a homodimer, but it is also capable of binding as a heterodimer with Stat1. In addition to serine phosphorylation, Stat3 is also phosphorylated at tyrosine 705 by JAK1 in response to cytokine stimulation. Stat3 is widely expressed and can bind to the sis-inducible element (SIE) site from the c-fos promoter. This site is similar to the GAS element that is present in IFN-g-induced genes. Thus, phosphorylation of S727 in Stat3 occurs in response to growth factors and cytokines, and is essential for normal transcription activity.
The 49/p-Stat3 monoclonal antibody recognizes the S727-phosphorylated form of Stat3 (isoform 1). The fluorochrome-conjugated formats have been evaluated using a human model system. However, the unconjugated form of this antibody (ABIN968868 or ABIN968869) is also effective for western blot analysis of human, mouse, and rat tissue.
Molecular Weight 92 kDa
Research Area Signaling, Receptors, Inflammation
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-STAT3 Antibody Target Details STAT3 Handling References for anti-STAT3 Antibody (ABIN968868) Images back to top
Restrictions For Research Use only

Handling

Product Details anti-STAT3 Antibody Target Details STAT3 Application Details References for anti-STAT3 Antibody (ABIN968868) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.

References for anti-STAT3 Antibody (ABIN968868)

Product Details anti-STAT3 Antibody Target Details STAT3 Application Details Handling Images back to top
Product cited in:

Kanai, Konda, Nakajima, Izumi, Kanda, Nanakin, Kubohara, Chiba: "Differentiation-inducing factor-1 (DIF-1) inhibits STAT3 activity involved in gastric cancer cell proliferation via MEK-ERK-dependent pathway." in: Oncogene, Vol. 22, Issue 4, pp. 548-54, 2003

Schuringa, Dekker, Vellenga, Kruijer: "Sequential activation of Rac-1, SEK-1/MKK-4, and protein kinase Cdelta is required for interleukin-6-induced STAT3 Ser-727 phosphorylation and transactivation." in: The Journal of biological chemistry, Vol. 276, Issue 29, pp. 27709-15, 2001

Smith, Crompton: "Expression of v-src in mammary epithelial cells induces transcription via STAT3." in: The Biochemical journal, Vol. 331 ( Pt 2), pp. 381-5, 1998

Fu, Zhang: "Transcription factor p91 interacts with the epidermal growth factor receptor and mediates activation of the c-fos gene promoter." in: Cell, Vol. 74, Issue 6, pp. 1135-45, 1993

Images

Product Details anti-STAT3 Antibody Target Details STAT3 Application Details Handling References for anti-STAT3 Antibody (ABIN968868) back to top
Supplier Images
Western Blotting (WB) image for anti-STAT3 antibody (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor)) (pSer727) (ABIN968868) A431 cells were either left untreated (left lane) or treated (right lane) with 100 ng...
Immunofluorescence (IF) image for anti-STAT3 antibody (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor)) (pSer727) (ABIN968868) A431 cells were serum starved and treated with EGF (100 ng/ml) for 5 minutes, then fi...
Immunofluorescence (IF) image for anti-STAT3 antibody (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor)) (pSer727) (ABIN968868) A431 cells were serum starved and treated with EGF (100 ng/ml) for 5 minutes, then fi...