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anti-Rat (Rattus) STAT3 antibody for Intracellular Staining

Recommended STAT3 Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) Antibodies
  • 1110034C02Rik
  • APRF
  • Aprf
  • aprf
  • AW109958
  • hies
  • HIES
  • stat3
  • wu:fc15d02
  • wu:fl59g06
  • Xstat3
  • z-Stat3
Epitope
pSer727
107
94
83
28
21
17
16
15
14
13
13
8
6
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
519
247
191
30
24
23
12
11
10
5
4
3
2
2
2
2
1
1
1
1
1
Host
Mouse
453
96
34
4
1
1
Clonality
Monoclonal
Conjugate
This STAT3 antibody is conjugated to Alexa Fluor 488
24
22
18
13
12
11
7
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Intracellular Staining (ICS)
485
233
206
74
69
65
46
25
23
12
12
12
9
7
3
3
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN1177194
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00087135227 ABIN1177193 ICS Alexa Fluor 647 Mouse IgG1 pSer727 Log in to see 49-p 2
0.00087135227 ABIN1177195 ICS PerCP-Cy5.5 Mouse IgG2a, kappa pTyr705 Log in to see 4-P 1
0.00087135227 ABIN1177192 ICS PE Mouse IgG1 pSer727 Log in to see 49-p 1

Top referenced anti-STAT3 antibody for Intracellular Staining

Similar anti-STAT3 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 436 Antibodies 190 Antibodies 148 Antibodies
Proximity Ligation Assay (PLA) 3 Antibodies
Intracellular Staining (ICS) 13 Antibodies 13 Antibodies 4 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies 2 Antibodies 2 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 53 Antibodies 49 Antibodies 29 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 61 Antibodies 31 Antibodies 28 Antibodies
Immunohistochemistry (IHC) 196 Antibodies 107 Antibodies 86 Antibodies

General

Antigen Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) Antibodies
Epitope pSer727
(107), (94), (83), (28), (21), (17), (16), (15), (14), (13), (13), (8), (6), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(519), (247), (191), (30), (24), (23), (12), (11), (10), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(453), (96), (34), (4), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This STAT3 antibody is conjugated to Alexa Fluor 488
(24), (22), (18), (13), (12), (11), (7), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Intracellular Staining (ICS)
(485), (233), (206), (74), (69), (65), (46), (25), (23), (12), (12), (12), (9), (7), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-STAT3 Antibody

Target Details STAT3 Application Details Handling References for anti-STAT3 Antibody (ABIN1177194) Images
Brand BD Phosflow™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human Stat3
Clone 49-p
Isotype IgG1

Target Details STAT3

Product Details anti-STAT3 Antibody Application Details Handling References for anti-STAT3 Antibody (ABIN1177194) Images back to top
Antigen
Alternative Name Stat3 (STAT3 Antibody Abstract)
Background The Stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Seven mammalian Stat proteins have been identified: Stat1-4, Stat5a, 5b, and Stat6. Stat3 is a 92-kDa protein that is activated as a DNA binding protein through cytokines, such as IL-6, and growth factors, such as EGF. Stat3 is phophorylated at serine 727 (S727) via the MAPK pathway. The S727 residue is located at a conserved Pro-X-Ser-Pro sequence, which is recognized by the protein kinase ERK. Activation through the S727 residue is thought to lead to initiation of transcription. Upon activation, Stat3 dimerizes, translocates to the nucleus, and binds DNA response elements thereby regulating gene expression. It appears that Stat3 binds to DNA as a homodimer, but it is also capable of binding as a heterodimer with Stat1. In addition to serine phosphorylation, Stat3 is also phosphorylated at tyrosine 705 by JAK1 in response to cytokine stimulation. Stat3 is widely expressed and can bind to the sis-inducible element (SIE) site from the c-fos promoter. This site is similar to the GAS element that is present in IFN-g-induced genes. Thus, phosphorylation of S727 in Stat3 occurs in response to growth factors and cytokines, and is essential for normal transcription activity. The 49/p-Stat3 monoclonal antibody recognizes the S727-phosphorylated form of Stat3 (isoform 1). The fluorochrome-conjugated formats have been evaluated using a human model system. However, the unconjugated form of this antibody (Cat. no. 612542 or 612543) is also effective for western blot analysis of human, mouse, and rat tissue.
Research Area Inflammation
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-STAT3 Antibody Target Details STAT3 Handling References for anti-STAT3 Antibody (ABIN1177194) Images back to top
Sample Volume 20 μL
Restrictions For Research Use only

Handling

Product Details anti-STAT3 Antibody Target Details STAT3 Application Details References for anti-STAT3 Antibody (ABIN1177194) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.

References for anti-STAT3 Antibody (ABIN1177194)

Product Details anti-STAT3 Antibody Target Details STAT3 Application Details Handling Images back to top
Product cited in:

Schuringa, Dekker, Vellenga, Kruijer: "Sequential activation of Rac-1, SEK-1/MKK-4, and protein kinase Cdelta is required for interleukin-6-induced STAT3 Ser-727 phosphorylation and transactivation." in: The Journal of biological chemistry, Vol. 276, Issue 29, pp. 27709-15, 2001

Smith, Crompton: "Expression of v-src in mammary epithelial cells induces transcription via STAT3." in: The Biochemical journal, Vol. 331 ( Pt 2), pp. 381-5, 1998

Fu, Zhang: "Transcription factor p91 interacts with the epidermal growth factor receptor and mediates activation of the c-fos gene promoter." in: Cell, Vol. 74, Issue 6, pp. 1135-45, 1993

Images

Product Details anti-STAT3 Antibody Target Details STAT3 Application Details Handling References for anti-STAT3 Antibody (ABIN1177194) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-STAT3 antibody (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor)) (pSer727) (Alexa Fluor 488) (ABIN1177194) anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (pSer727) antibody (Alexa Fluor 488)