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anti-Xenopus laevis STAT3 antibody for Western Blotting

Recommended STAT3 Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) Antibodies
  • 1110034C02Rik
  • APRF
  • Aprf
  • aprf
  • AW109958
  • hies
  • HIES
  • stat3
  • wu:fc15d02
  • wu:fl59g06
  • Xstat3
  • z-Stat3
Epitope
N-Term
112
95
85
28
21
17
16
15
14
13
13
13
8
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
545
266
208
29
23
23
13
11
9
4
4
3
3
2
2
2
1
1
1
1
1
1
Host
Rabbit
465
109
35
4
1
1
Clonality
Polyclonal
Conjugate
This STAT3 antibody is un-conjugated
24
22
18
13
12
11
8
5
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
503
239
216
80
76
70
53
32
23
13
12
12
9
7
3
3
2
2
2
2
1
1
1
1
Supplier
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Catalog No. ABIN2780479
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13341247 ABIN2792239 IHC WB Rabbit Middle Region Log in to see Polyclonal 2

Top referenced anti-STAT3 antibody for Western Blotting

Similar anti-STAT3 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine) Chicken Cow (Bovine) Pig (Porcine) Xenopus laevis Zebrafish (Danio rerio)
Western Blotting (WB) 454 Antibodies 202 Antibodies 158 Antibodies 20 Antibodies 8 Antibodies 15 Antibodies 15 Antibodies 2 Antibodies 3 Antibodies
Proximity Ligation Assay (PLA) 3 Antibodies
Intracellular Staining (ICS) 13 Antibodies 13 Antibodies 4 Antibodies 2 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies 2 Antibodies 2 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 9 Antibodies 54 Antibodies

General

Antigen Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) Antibodies
Epitope N-Term
(112), (95), (85), (28), (21), (17), (16), (15), (14), (13), (13), (13), (8), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(545), (266), (208), (29), (23), (23), (13), (11), (9), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(465), (109), (35), (4), (1), (1)
Clonality
Polyclonal
Conjugate This STAT3 antibody is un-conjugated
(24), (22), (18), (13), (12), (11), (8), (5), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(503), (239), (216), (80), (76), (70), (53), (32), (23), (13), (12), (12), (9), (7), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-STAT3 Antibody

Target Details STAT3 Application Details Handling References for anti-STAT3 Antibody (ABIN2780479) Images
Blocking Peptide Blocking peptide for this product available: ABIN984529
Sequence AQWNQLQQLD TRYLEQLHQL YSDSFPMELR QFLAPWIESQ DWAYAASKES
Predicted Reactivity Bovine : 100 %, Chicken : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %, Zebrafish : 70 %
Characteristics This is a rabbit polyclonal antibody against STAT3. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human STAT3

Target Details STAT3

Product Details anti-STAT3 Antibody Application Details Handling References for anti-STAT3 Antibody (ABIN2780479) Images back to top
Antigen
Alternative Name STAT3 (STAT3 Antibody Abstract)
Background STAT3 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. It mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein.The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described.
Molecular Weight 88 kDa
Gene ID 6774
NCBI Accession NP_003141, NM_003150
UniProt B5BTZ6
Research Area Embryogenesis, Embryonic stem cells, Transcription Factors, Signaling, Receptors, Inflammation
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-STAT3 Antibody Target Details STAT3 Handling References for anti-STAT3 Antibody (ABIN2780479) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 769 AA

Restrictions For Research Use only

Handling

Product Details anti-STAT3 Antibody Target Details STAT3 Application Details References for anti-STAT3 Antibody (ABIN2780479) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-STAT3 Antibody (ABIN2780479)

Product Details anti-STAT3 Antibody Target Details STAT3 Application Details Handling Images back to top
Product cited in:

Deng, Chen, Li, Liu, Niu, Zhou, Li, Ke, Mo et al.: "Bone marrow mesenchymal stromal cells with support of bispecific antibody and ultrasound-mediated microbubbles prevent myocardial fibrosis via the signal transducer and activators of transcription ..." in: Cytotherapy, Vol. 13, Issue 4, pp. 431-40, 2011

Background publications

Ohbayashi, Kawakami, Muromoto, Togi, Ikeda, Kamitani, Sekine, Honjoh, Matsuda: "The IL-6 family of cytokines modulates STAT3 activation by desumoylation of PML through SENP1 induction." in: Biochemical and biophysical research communications, Vol. 371, Issue 4, pp. 823-8, 2008

Images

Product Details anti-STAT3 Antibody Target Details STAT3 Application Details Handling References for anti-STAT3 Antibody (ABIN2780479) back to top
Supplier Images
Western Blotting (WB) image for anti-STAT3 antibody (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor)) (N-Term) (ABIN2780479) WB Suggested Anti-STAT3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Immunohistochemistry (IHC) image for anti-STAT3 antibody (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor)) (N-Term) (ABIN2780479) anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (N-Term) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-STAT3 antibody (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor)) (N-Term) (ABIN2780479) anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (N-Term) antibody (Image 3)