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anti-Dog (Canine) STAT4 antibody for Enzyme Immunoassay

Recommended STAT4 Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 4 (STAT4) Antibodies
  • SLEB11
Epitope
Internal Region, AA 613-627
69
29
15
13
13
11
7
6
6
6
5
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Pig (Porcine)
265
103
78
7
7
4
3
2
2
2
1
Host
Goat
179
70
25
2
2
Clonality
Polyclonal
Conjugate
This STAT4 antibody is un-conjugated
9
9
9
8
7
6
4
3
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
198
103
81
44
36
34
24
22
7
6
3
3
2
2
1
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Catalog No. ABIN499124
$ 379.50
Plus shipping costs $45.00

Top referenced anti-STAT4 antibody for Enzyme Immunoassay

Similar anti-STAT4 Antibodies

Application / Reactivity Human Dog (Canine) Pig (Porcine)
Western Blotting (WB) 188 Antibodies 8 Antibodies 8 Antibodies
Luminex Assay (LMNX) 6 Antibodies
Intracellular Staining (ICS) 7 Antibodies
Immunoprecipitation (IP) 30 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 36 Antibodies
Immunohistochemistry (IHC) 4 Antibodies 105 Antibodies 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunofluorescence (IF) 44 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunochromatography (IC) 1 Antibodies
Flow Cytometry (FACS) 25 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies 1 Antibodies

General

Antigen Signal Transducer and Activator of Transcription 4 (STAT4) Antibodies
Epitope Internal Region, AA 613-627
(69), (29), (15), (13), (13), (11), (7), (6), (6), (6), (5), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Pig (Porcine)
(265), (103), (78), (7), (7), (4), (3), (2), (2), (2), (1)
Host Goat
(179), (70), (25), (2), (2)
Clonality
Polyclonal
Conjugate This STAT4 antibody is un-conjugated
(9), (9), (9), (8), (7), (6), (4), (3), (2), (2), (2), (2), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(198), (103), (81), (44), (36), (34), (24), (22), (7), (6), (3), (3), (2), (2), (1)
Pubmed 1 reference available
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Product Details anti-STAT4 Antibody

Target Details STAT4 Application Details Handling References for anti-STAT4 Antibody (ABIN499124) Images
Sequence C-DHSESGEVRF HSVEP
Specificity Recognizes STAT4 (aa 613-627).
Cross-Reactivity (Details) Species reactivity (expected):Canine, Pig.
Species reactivity (tested):Human.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
Immunogen Peptide with sequence from the internal region (aa 613-627) of the protein sequence according to Human STAT4 (NP_003142.1.) Genename: STAT4

Target Details STAT4

Product Details anti-STAT4 Antibody Application Details Handling References for anti-STAT4 Antibody (ABIN499124) Images back to top
Antigen
Alternative Name STAT4 (STAT4 Antibody Abstract)
Background The STAT 4 protein is a transcription factor activated by hormone and cytokines. STAT 4 is most similar to STAT 1 (52 % ) and STAT 3 (47 %). Functionally STAT 4 is similar to other STAT family members in that it can be tyrosine phosphorylated by JAK 1 or JAK 2. STAT 4 forms homodimers and heterodimers with related STAT family members. Tyrosine phosphorylated STAT 4 can bind the interferon g activated site (GAS), and serine phosphorylation is also required for maximal transcriptional activity. STAT 4 expression is restricted to the thymus, spleen, and testis.Synonyms: Signal transducer and activator of transcription 4
Gene ID 6775
NCBI Accession NP_003142
UniProt Q14765
Research Area Chromatin and Nuclear Signaling, Transcription Factors, Signaling, Receptors, Inflammation
Pathways JAK-STAT Signaling

Application Details

Product Details anti-STAT4 Antibody Target Details STAT4 Handling References for anti-STAT4 Antibody (ABIN499124) Images back to top
Application Notes Peptide ELISA: Limit Dilution: 1/32000. Western blot: 0.2-0.5 μg/mL. This antibody detects a band of approx. 85 kDa in transfectedHEK293 transiently expressing full-length Human STAT4 (MYC and DYKDDDDK tagged). No bands were observed in mock-transfected HEK293 and the same band was observedusing anti-myc tag antibody.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-STAT4 Antibody Target Details STAT4 Application Details References for anti-STAT4 Antibody (ABIN499124) Images back to top
Concentration 0.5 mg/mL
Buffer Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-STAT4 Antibody (ABIN499124)

Product Details anti-STAT4 Antibody Target Details STAT4 Application Details Handling Images back to top
Background publications

Good, Thieu, Mathur, Yu, Stritesky, Yeh, OMalley, Perumal, Kaplan: "Temporal induction pattern of STAT4 target genes defines potential for Th1 lineage-specific programming." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 6, pp. 3839-47, 2009

Images

Product Details anti-STAT4 Antibody Target Details STAT4 Application Details Handling References for anti-STAT4 Antibody (ABIN499124) back to top
Supplier Images
Western Blotting (WB) image for anti-STAT4 antibody (Signal Transducer and Activator of Transcription 4) (Internal Region) (ABIN499124) HEK293 lysate (10 µg protein in RIPA buffer) overexpressing Human STAT4 with C-termin...