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anti-Human STAT4 antibody for Flow Cytometry

Recommended STAT4 Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 4 (STAT4) Antibodies
  • SLEB11
Epitope
pTyr693
69
29
15
13
13
11
7
6
6
6
5
4
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
270
103
79
8
8
4
3
2
2
2
1
Host
Mouse
181
70
28
2
2
Clonality
Monoclonal
Conjugate
This STAT4 antibody is conjugated to PE
9
9
8
8
7
6
4
3
2
2
2
2
2
2
2
Application
Flow Cytometry (FACS), Intracellular Staining (ICS)
204
106
83
46
37
36
24
22
6
6
4
4
3
2
1
Supplier
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Available images

Catalog No. ABIN2680985
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30391768 ABIN2680987 FACS ICS APC Mouse IgG1 kappa pTyr693 Log in to see 4LURPIE
0.0010469793 ABIN1501176 FACS IF WB Mouse IgG1 Log in to see 1G9
0.0010469793 ABIN1501177 FACS IHC (p) WB Mouse IgG1 Log in to see 1B1
0.0010469793 ABIN1501174 FACS IF IHC (p) WB Mouse IgG1 Log in to see 3B6
0.0010469793 ABIN1501175 FACS IF IHC (p) WB Mouse IgG1 Log in to see 2F3
0.0010469793 ABIN1501178 FACS IHC (p) WB Mouse IgG1 Log in to see 4H6
0.0010469793 ABIN2665369 FACS Mouse IgG2b kappa Log in to see 15A1B41 4
0.0010469793 ABIN445103 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG Ser721 Log in to see EP1900Y
0.0010469793 ABIN2572824 FACS Mouse IgG1 Log in to see 4F9
0.0010469793 ABIN2572822 FACS Mouse IgG1 Log in to see 2G5
0.0010469793 ABIN2572823 FACS Mouse IgG1 Log in to see 2B4
0.0010469793 ABIN2152343 FACS IF IHC WB Mouse IgG1 Log in to see 3B6
0.0010469793 ABIN2152341 FACS IHC WB Mouse IgG1 Log in to see 1B1
0.0010469793 ABIN2152346 FACS WB Mouse IgG1 Log in to see 4H6
0.0010469793 ABIN2152340 FACS IF WB Mouse IgG1 Log in to see 1G9
0.0010469793 ABIN2152344 FACS IHC WB Mouse IgG1 Log in to see 2F3
0.0010469793 ABIN2670163 FACS IHC WB Mouse IgG1 Log in to see OTI1B1
0.0010469793 ABIN2670162 FACS IF WB Mouse IgG1 Log in to see OTI1G9
0.0010469793 ABIN2670166 FACS IF WB Mouse IgG1 Log in to see OTI2G5
0.0010469793 ABIN2670167 FACS IHC WB Mouse IgG1 Log in to see OTI4H6

Similar anti-STAT4 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 15 Antibodies 79 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 4 Antibodies
Flow Cytometry (FACS) 3 Antibodies 26 Antibodies
Gel Shift (GS) 2 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 5 Antibodies
Immunofluorescence (IF) 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 38 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies
Immunoprecipitation (IP) 22 Antibodies
Intracellular Staining (ICS) 5 Antibodies
Western Blotting (WB) 72 Antibodies
Immunochromatography (IC) 1 Antibodies

General

Antigen Signal Transducer and Activator of Transcription 4 (STAT4) Antibodies
Epitope pTyr693
(69), (29), (15), (13), (13), (11), (7), (6), (6), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(270), (103), (79), (8), (8), (4), (3), (2), (2), (2), (1)
Host Mouse
(181), (70), (28), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This STAT4 antibody is conjugated to PE
(9), (9), (8), (8), (7), (6), (4), (3), (2), (2), (2), (2), (2), (2), (2)
Application Flow Cytometry (FACS), Intracellular Staining (ICS)
(204), (106), (83), (46), (37), (36), (24), (22), (6), (6), (4), (4), (3), (2), (1)
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Product Details anti-STAT4 Antibody

Target Details STAT4 Application Details Handling Images
Specificity This 4LURPIE monoclonal antibody recognizes human and mouse signal transducer and activator of transcription 4 (STAT4) when phosphorylated on tyrosine 693 (Y693). STAT proteins are activated by ligand binding to cytokine receptors that associate with Janus kinase (JAK) family members. Following their phosphorylation by JAK proteins, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. STAT4 is activated by IL-12 or type 1 IFN (IFN alpha, IFN beta). STAT4 is required for optimal Th1 differentiation in vivo. Interestingly, although IFN alpha can lead to phosphorylation of STAT4 in mouse T cells, the phosphorylation is much weaker than that induced by IL-12 and is transient. Thus, IFN alpha phosphorylation of STAT4 does not contribute to STAT4-mediated Th1 development or IFN gamma production by Th1 cells in mice. In contrast, IFN alpha does promote Th1 development and function of human CD4+ T cells.

Specificity of this 4LURPIE clone was determined by ELISA and flow cytometry.
Characteristics This 4LURPIE monoclonal antibody recognizes human and mouse signal transducer and activator of transcription 4 (STAT4) when phosphorylated on tyrosine 693 (Y693). STAT proteins are activated by ligand binding to cytokine receptors that associate with Janus kinase (JAK) family members. Following their phosphorylation by JAK proteins, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. STAT4 is activated by IL-12 or type 1 IFN (IFN alpha, IFN beta). STAT4 is required for optimal Th1 differentiation in vivo. Interestingly, although IFN alpha can lead to phosphorylation of STAT4 in mouse T cells, the phosphorylation is much weaker than that induced by IL-12 and is transient. Thus, IFN alpha phosphorylation of STAT4 does not contribute to STAT4-mediated Th1 development or IFN gamma production by Th1 cells in mice. In contrast, IFN alpha does promote Th1 development and function of human CD4+ T cells. Specificity of this 4LURPIE clone was determined by ELISA and flow cytometry.
Synonyms: Synonyms: Signal transducer and activator of transcription 4
Clone 4LURPIE
Isotype IgG1 kappa

Target Details STAT4

Product Details anti-STAT4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name STAT4 (STAT4 Antibody Abstract)
Research Area Chromatin and Nuclear Signaling, Transcription Factors, Signaling, Receptors, Inflammation
Pathways JAK-STAT Signaling

Application Details

Product Details anti-STAT4 Antibody Target Details STAT4 Handling Images back to top
Application Notes Applications reported: This 4LURPIE antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications tested: This 4LURPIE antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10 5 to 10 8 cells/test. Staining Protocol: We recommend using Protocol C: Two-step protocol: Fixation/Methanol. Protocol A: Two- step protocol: intracellular (cytoplasmic) proteins and Protocol B: One-step protocol: intracellular (nuclear) proteins cannot be used. All Protocols can be found in the Flow Cytometry Protocols: "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the Best Protocols Section under the Resources tab online.
Restrictions For Research Use only

Handling

Product Details anti-STAT4 Antibody Target Details STAT4 Application Details Images back to top
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Handling Advice Do not freeze. Light-sensitive material.
Storage 4 °C
Storage Comment Store at 2-8°C.

Images

Product Details anti-STAT4 Antibody Target Details STAT4 Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-STAT4 antibody (Signal Transducer and Activator of Transcription 4) (pTyr693) (PE) (ABIN2680985) anti-Signal Transducer and Activator of Transcription 4 (STAT4) (pTyr693) antibody (PE)