You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Rat (Rattus) STAT4 antibody for Immunoprecipitation

Recommended STAT4 Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 4 (STAT4) Antibodies
  • SLEB11
Epitope
AA 730-749
69
29
15
13
13
11
7
6
6
6
5
4
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
265
102
77
8
8
4
3
2
2
2
1
Host
Rabbit
178
70
26
2
2
Clonality
Polyclonal
Conjugate
This STAT4 antibody is un-conjugated
9
9
9
8
7
6
4
3
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
198
103
81
44
35
34
24
22
7
6
3
3
2
2
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN117931
$ 456.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089538493 ABIN2506009 IF IP IHC ELISA WB Rabbit IgG Tyr693 Log in to see Polyclonal
0.00089538493 ABIN3182151 ELISA IHC IP WB Rabbit IgG pTyr693 Log in to see Polyclonal
0.00089538493 ABIN3187085 ELISA IHC IP WB Rabbit IgG Tyr1243 Log in to see Polyclonal
0.00089538493 ABIN1845630 IP IHC WB Rabbit pTyr693 Log in to see Polyclonal
0.00089538493 ABIN1845632 IP IHC WB Rabbit Log in to see Polyclonal
0.00089538493 ABIN2858213 IP IHC WB Rabbit pTyr693 Log in to see Polyclonal
0.00089538493 ABIN2859043 IP IHC WB Rabbit Log in to see Polyclonal
0.00089538493 ABIN184420 IHC (fro) IP WB Rabbit Log in to see Polyclonal

Top referenced anti-STAT4 antibody for Immunoprecipitation

Similar anti-STAT4 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 10 Antibodies 15 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 1 Antibodies
Immunofluorescence (IF) 4 Antibodies 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies 22 Antibodies
Immunohistochemistry (IHC) 31 Antibodies 37 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies 11 Antibodies
Immunoprecipitation (IP) 9 Antibodies
Western Blotting (WB) 53 Antibodies
Flow Cytometry (FACS) 3 Antibodies
Gel Shift (GS) 2 Antibodies

General

Antigen Signal Transducer and Activator of Transcription 4 (STAT4) Antibodies
Epitope AA 730-749
(69), (29), (15), (13), (13), (11), (7), (6), (6), (6), (5), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(265), (102), (77), (8), (8), (4), (3), (2), (2), (2), (1)
Host Rabbit
(178), (70), (26), (2), (2)
Clonality
Polyclonal
Conjugate This STAT4 antibody is un-conjugated
(9), (9), (9), (8), (7), (6), (4), (3), (2), (2), (2), (2), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
(198), (103), (81), (44), (35), (34), (24), (22), (7), (6), (3), (3), (2), (2), (1)
Pubmed 8 references available
Supplier Log in to see

Product Details anti-STAT4 Antibody

Target Details STAT4 Application Details Handling References for anti-STAT4 Antibody (ABIN117931) Images
Sequence Peptide sequence: R-E-N-L-S-P-T-T-I-E-T-A-M-N-S-P-Y-S-A-E Protein sequence: Mouse STAT4 protein is composed of 749 amino acids (85.9 kDa) 1 msqwnqvqql eikfleqvdq fyddnfpmei rhllaqwiet dwevasnne tmatillqnl 61 liqldeqlgr vskeknllli hnlkrirkvl qgkfhgnpmh vavvisnclr eerrilaaan 121 mpiqgpleks lqsssvserq rnvehkvsai knsvqmteqd tkyledlqde fdyryktiqt 181 mdqgdknsil vnqevltllq emlnsldfkr kealskmtqi vnetdllmns mlleelqdwk 241 krqqiacigg plhngldqlq ncftllaesl fqlrqqlekl qeqstkmtye gdpipaqrah 301 lleratfliy nlfknsfvve qpcmpthpq rpmvlktliq tvklrllik lpelnyqvkv 361 kasidknvst lsnrrfvlcg thvkamssee ssngslsvef rhlqpkemkc stgskgnegc 421 hmvteelhsi tfetqiclyg ltinletssl pvvmisnvsq lpnawasiiw ynvstndsqn 481 lvffnnppsv tlgqllevms wqfssyvgrg lnseqlnmla ekltvqsnyn dghltwakfc 541 kehlpgktft fwtwleaild likkhilplw idgyimgfvs kekerlllkd kmpgtfllrf 601 seshlggitf twvdqsenge vrfhsvepyn kgrlsalafa dilrdykvim aenipenplk 661 ylypdipkdk afgkhyssqp cevsrpterg dkgyvpsvfi pistirsdst epqspsdllp 721 mspsayavlr enlspttiet amnspysae
Specificity This antibody was prepared from whole rabbit antiserum by delipidation and defibrination. Reactivity occurs against mouse STAT4 protein. Cross reactivity with closely related STAT family members has not been determined. Cross reactivity is expected against human STAT4 protein based on a 95 % homology of the amino acid sequence of the immunogen between human and mouse. A single amino acid change is reported (from N to K). Cross reactivity is expected against rat STAT4.
Purification Delipidation and defibrination.
Immunogen This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 730-749 of Mouse STAT -4. Swiss Prot accession number P42228.

Target Details STAT4

Product Details anti-STAT4 Antibody Application Details Handling References for anti-STAT4 Antibody (ABIN117931) Images back to top
Antigen
Alternative Name STAT4 (STAT4 Antibody Abstract)
Background Signal transducer and activator of transcription 4 (STAT4) carries out a dual function of signal transduction and activation of transcription. STAT4 forms a homodimer or a heterodimer with related transcription factor STAT family members. STAT4 has a cytoplasmic localization and is translocated into the nucleus in response to activation by JAK, which phosphorylates STAT4 at a tyrosine residue. Various ligands, including interferons and growth factors, induce activation of the JAK kinases, which induce STAT activation. IL-12 has also been identified as an activator of STAT4. Serine phosphorylation is also required for maximal transcriptional activity. The highest level of STAT4 expression is observed in testis and in myeloid cells.Synonyms: Signal transducer and activator of transcription 4
Gene ID 6775
NCBI Accession NP_003142
UniProt Q14765
Research Area Chromatin and Nuclear Signaling, Transcription Factors, Signaling, Receptors, Inflammation
Pathways JAK-STAT Signaling

Application Details

Product Details anti-STAT4 Antibody Target Details STAT4 Handling References for anti-STAT4 Antibody (ABIN117931) Images back to top
Application Notes This antibody has been tested for use in ELISA, Western blotting and Immunoprecipitation. Reactivity in other immunoassays is unknown. A HeLa whole cell lysate or mouse testislysate are suitable for use as a positive control. Recommended Dilutions: This product was assayed by western blotting afterImmunoprecipitation of mouse STAT4 from a lysate containing over expressed protein. A 1: 5,000 dilution shows reactivity with a band at 85 kDa corresponding to mouse STAT4. Use 5 μL for Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-STAT4 Antibody Target Details STAT4 Application Details References for anti-STAT4 Antibody (ABIN117931) Images back to top
Format Liquid
Concentration 85 mg/mL (by refractometry)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01 % (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid.

References for anti-STAT4 Antibody (ABIN117931)

Product Details anti-STAT4 Antibody Target Details STAT4 Application Details Handling Images back to top
Background publications

OSullivan, Chang, Yu, Kaplan: "STAT4 is required for interleukin-12-induced chromatin remodeling of the CD25 locus." in: The Journal of biological chemistry, Vol. 279, Issue 8, pp. 7339-45, 2004

Baum, Trabanco, Montalban, Micallef, Zhong, Meunier, Suhling, Phillips, White, Williams, Barrett, Hoffman: "Synthesis and reactions of aminoporphyrazines with annulated five- and seven-membered rings." in: The Journal of organic chemistry, Vol. 68, Issue 5, pp. 1665-70, 2003

Freudenberg, Merlin, Kalis, Chvatchko, Stübig, Galanos: "Cutting edge: a murine, IL-12-independent pathway of IFN-gamma induction by gram-negative bacteria based on STAT4 activation by Type I IFN and IL-18 signaling." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 4, pp. 1665-8, 2002

Schindler, Lutz, Röllinghoff, Bogdan: "The production of IFN-gamma by IL-12/IL-18-activated macrophages requires STAT4 signaling and is inhibited by IL-4." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 5, pp. 3075-82, 2001

Poltorak, Merlin, Nielsen, Sandra, Smirnova, Schupp, Boehm, Galanos, Freudenberg: "A point mutation in the IL-12R beta 2 gene underlies the IL-12 unresponsiveness of Lps-defective C57BL/10ScCr mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 4, pp. 2106-11, 2001

Matikainen, Sareneva, Ronni, Lehtonen, Koskinen, Julkunen: "Interferon-alpha activates multiple STAT proteins and upregulates proliferation-associated IL-2Ralpha, c-myc, and pim-1 genes in human T cells." in: Blood, Vol. 93, Issue 6, pp. 1980-91, 1999

Yamamoto, Quelle, Thierfelder, Kreider, Gilbert, Jenkins, Copeland, Silvennoinen, Ihle: "Stat4, a novel gamma interferon activation site-binding protein expressed in early myeloid differentiation." in: Molecular and cellular biology, Vol. 14, Issue 7, pp. 4342-9, 1994

Zhong, Wen, Darnell: "Stat3 and Stat4: members of the family of signal transducers and activators of transcription." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 11, pp. 4806-10, 1994

Images

Product Details anti-STAT4 Antibody Target Details STAT4 Application Details Handling References for anti-STAT4 Antibody (ABIN117931) back to top
Supplier Images
Western Blotting (WB) image for anti-STAT4 antibody (Signal Transducer and Activator of Transcription 4) (AA 730-749) (ABIN117931) ",Western blot following immunoprecipitation of STAT4 using ROCKLAND Immunochemic...