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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||10 Antibodies||15 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||1 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies||1 Antibodies|
|Immunofluorescence (IF)||4 Antibodies||4 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||23 Antibodies||23 Antibodies|
|Immunohistochemistry (IHC)||31 Antibodies||37 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||7 Antibodies||11 Antibodies|
|Immunoprecipitation (IP)||9 Antibodies|
|Western Blotting (WB)||53 Antibodies|
|Flow Cytometry (FACS)||3 Antibodies|
|Gel Shift (GS)||2 Antibodies|
|Antigen||Signal Transducer and Activator of Transcription 4 (STAT4) Antibodies|
|Epitope||AA 730-749 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This STAT4 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
|8 references available|
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Product Details anti-STAT4 AntibodyTarget Details STAT4 Application Details Handling References for anti-STAT4 Antibody (ABIN117931) Images
|Sequence||Peptide sequence: R-E-N-L-S-P-T-T-I-E-T-A-M-N-S-P-Y-S-A-E Protein sequence: Mouse STAT4 protein is composed of 749 amino acids (85.9 kDa) 1 msqwnqvqql eikfleqvdq fyddnfpmei rhllaqwiet dwevasnne tmatillqnl 61 liqldeqlgr vskeknllli hnlkrirkvl qgkfhgnpmh vavvisnclr eerrilaaan 121 mpiqgpleks lqsssvserq rnvehkvsai knsvqmteqd tkyledlqde fdyryktiqt 181 mdqgdknsil vnqevltllq emlnsldfkr kealskmtqi vnetdllmns mlleelqdwk 241 krqqiacigg plhngldqlq ncftllaesl fqlrqqlekl qeqstkmtye gdpipaqrah 301 lleratfliy nlfknsfvve qpcmpthpq rpmvlktliq tvklrllik lpelnyqvkv 361 kasidknvst lsnrrfvlcg thvkamssee ssngslsvef rhlqpkemkc stgskgnegc 421 hmvteelhsi tfetqiclyg ltinletssl pvvmisnvsq lpnawasiiw ynvstndsqn 481 lvffnnppsv tlgqllevms wqfssyvgrg lnseqlnmla ekltvqsnyn dghltwakfc 541 kehlpgktft fwtwleaild likkhilplw idgyimgfvs kekerlllkd kmpgtfllrf 601 seshlggitf twvdqsenge vrfhsvepyn kgrlsalafa dilrdykvim aenipenplk 661 ylypdipkdk afgkhyssqp cevsrpterg dkgyvpsvfi pistirsdst epqspsdllp 721 mspsayavlr enlspttiet amnspysae|
|Specificity||This antibody was prepared from whole rabbit antiserum by delipidation and defibrination. Reactivity occurs against mouse STAT4 protein. Cross reactivity with closely related STAT family members has not been determined. Cross reactivity is expected against human STAT4 protein based on a 95 % homology of the amino acid sequence of the immunogen between human and mouse. A single amino acid change is reported (from N to K). Cross reactivity is expected against rat STAT4.|
|Purification||Delipidation and defibrination.|
|Immunogen||This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 730-749 of Mouse STAT -4. Swiss Prot accession number P42228.|
Target Details STAT4Product Details anti-STAT4 Antibody Application Details Handling References for anti-STAT4 Antibody (ABIN117931) Images back to top
|Alternative Name||STAT4 (STAT4 Antibody Abstract)|
|Background||Signal transducer and activator of transcription 4 (STAT4) carries out a dual function of signal transduction and activation of transcription. STAT4 forms a homodimer or a heterodimer with related transcription factor STAT family members. STAT4 has a cytoplasmic localization and is translocated into the nucleus in response to activation by JAK, which phosphorylates STAT4 at a tyrosine residue. Various ligands, including interferons and growth factors, induce activation of the JAK kinases, which induce STAT activation. IL-12 has also been identified as an activator of STAT4. Serine phosphorylation is also required for maximal transcriptional activity. The highest level of STAT4 expression is observed in testis and in myeloid cells.Synonyms: Signal transducer and activator of transcription 4|
|Research Area||Chromatin and Nuclear Signaling, Transcription Factors, Signaling, Receptors, Inflammation|
Application DetailsProduct Details anti-STAT4 Antibody Target Details STAT4 Handling References for anti-STAT4 Antibody (ABIN117931) Images back to top
This antibody has been tested for use in ELISA, Western blotting and Immunoprecipitation. Reactivity in other immunoassays is unknown. A HeLa whole cell lysate or mouse testislysate are suitable for use as a positive control. Recommended Dilutions: This product was assayed by western blotting afterImmunoprecipitation of mouse STAT4 from a lysate containing over expressed protein. A 1: 5,000 dilution shows reactivity with a band at 85 kDa corresponding to mouse STAT4. Use 5 μL for Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-STAT4 Antibody Target Details STAT4 Application Details References for anti-STAT4 Antibody (ABIN117931) Images back to top
|Concentration||85 mg/mL (by refractometry)|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01 % (w/v) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Dilute only prior to immediate use. Avoid cycles of freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid.|
References for anti-STAT4 Antibody (ABIN117931)Product Details anti-STAT4 Antibody Target Details STAT4 Application Details Handling Images back to top
OSullivan, Chang, Yu et al.: "STAT4 is required for interleukin-12-induced chromatin remodeling of the CD25 locus." in: The Journal of biological chemistry, Vol. 279, Issue 8, pp. 7339-45, 2004 (PubMed).
Baum, Trabanco, Montalban et al.: "Synthesis and reactions of aminoporphyrazines with annulated five- and seven-membered rings." in: The Journal of organic chemistry, Vol. 68, Issue 5, pp. 1665-70, 2003 (PubMed).
Freudenberg, Merlin, Kalis et al.: "Cutting edge: a murine, IL-12-independent pathway of IFN-gamma induction by gram-negative bacteria based on STAT4 activation by Type I IFN and IL-18 signaling." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 4, pp. 1665-8, 2002 (PubMed).
Poltorak, Merlin, Nielsen et al.: "A point mutation in the IL-12R beta 2 gene underlies the IL-12 unresponsiveness of Lps-defective C57BL/10ScCr mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 4, pp. 2106-11, 2001 (PubMed).
Schindler, Lutz, Röllinghoff et al.: "The production of IFN-gamma by IL-12/IL-18-activated macrophages requires STAT4 signaling and is inhibited by IL-4." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 5, pp. 3075-82, 2001 (PubMed).
Matikainen, Sareneva, Ronni et al.: "Interferon-alpha activates multiple STAT proteins and upregulates proliferation-associated IL-2Ralpha, c-myc, and pim-1 genes in human T cells." in: Blood, Vol. 93, Issue 6, pp. 1980-91, 1999 (PubMed).
Yamamoto, Quelle, Thierfelder et al.: "Stat4, a novel gamma interferon activation site-binding protein expressed in early myeloid differentiation." in: Molecular and cellular biology, Vol. 14, Issue 7, pp. 4342-9, 1994 (PubMed).
Zhong, Wen, Darnell: "Stat3 and Stat4: members of the family of signal transducers and activators of transcription." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 11, pp. 4806-10, 1994 (PubMed).
ImagesProduct Details anti-STAT4 Antibody Target Details STAT4 Application Details Handling References for anti-STAT4 Antibody (ABIN117931) back to top