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anti-Human STAT5A antibody for Enzyme Immunoassay

Recommended STAT5A Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 5A (STAT5A) Antibodies
  • AA959963
  • MGF
  • STAT5
  • Stat5
  • STAT5A
  • STAT5A/MGF
  • STATA5
Epitope
pSer726
86
52
42
16
15
13
9
6
6
6
5
5
5
4
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
405
195
157
28
21
16
13
11
6
1
Host
Rabbit
321
97
10
Clonality
Polyclonal
Conjugate
This STAT5A antibody is un-conjugated
12
11
10
8
6
5
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
323
195
132
55
54
51
33
22
18
17
12
8
6
4
4
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN358237
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088294124 ABIN358238 EIA IHC (p) WB Rabbit Ig pTyr694 Log in to see Polyclonal 5
0.00088294124 ABIN401413 EIA IHC (p) WB Rabbit IgG Tyr694 Log in to see Polyclonal 4
0.00088294124 ABIN400812 EIA IF IHC (p) WB Mouse IgG1 Tyr694 Log in to see 5F6-F1 4
0.00088294124 ABIN271829 EIA IHC (p) WB Rabbit Log in to see Polyclonal
0.00088294124 ABIN401412 EIA WB Rabbit IgG C-Term Log in to see Polyclonal 1
0.00088294124 ABIN372786 EIA IHC (p) WB Rabbit IgG Tyr694 Log in to see Polyclonal
0.00088294124 ABIN126900 EIA IHC (fro) IF WB Mouse IgG1 pTyr695, pTyr699 Log in to see 5G4
0.00088294124 ABIN641226 CA DB EIA IA ISt ELISA WB Rabbit IgG pTyr694 Log in to see Polyclonal

Top referenced anti-STAT5A antibody for Enzyme Immunoassay

Similar anti-STAT5A Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 2 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 18 Antibodies
ELISA 119 Antibodies
Enzyme Immunoassay (EIA) 9 Antibodies
Flow Cytometry (FACS) 54 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 11 Antibodies
Immunofluorescence (IF) 48 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 186 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 55 Antibodies
Immunoprecipitation (IP) 29 Antibodies
Immunostaining (ISt) 1 Antibodies
Intracellular Staining (ICS) 17 Antibodies
Luminex Assay (LMNX) 6 Antibodies

General

Antigen Signal Transducer and Activator of Transcription 5A (STAT5A) Antibodies
Epitope pSer726
(86), (52), (42), (16), (15), (13), (9), (6), (6), (6), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(405), (195), (157), (28), (21), (16), (13), (11), (6), (1)
Host Rabbit
(321), (97), (10)
Clonality
Polyclonal
Conjugate This STAT5A antibody is un-conjugated
(12), (11), (10), (8), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(323), (195), (132), (55), (54), (51), (33), (22), (18), (17), (12), (8), (6), (4), (4), (2), (2), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-STAT5A Antibody

Target Details STAT5A Application Details Handling References for anti-STAT5A Antibody (ABIN358237) Images
Specificity This antibody detects STAT5a pSer726
Purification Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Ser726 of human STAT5a.
Isotype Ig

Target Details STAT5A

Product Details anti-STAT5A Antibody Application Details Handling References for anti-STAT5A Antibody (ABIN358237) Images back to top
Antigen
Alternative Name STAT5 / STAT5A (STAT5A Antibody Abstract)
Background STAT5a is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this protein is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this protein in cells.Synonyms: MGF, Mammary gland factor, STAT-5, STAT-5A, Signal transducer and activator of transcription 5A
Molecular Weight 90647 Da
Gene ID 6776, 9606
UniProt P42229
Pathways JAK-STAT Signaling, RTK Signaling

Application Details

Product Details anti-STAT5A Antibody Target Details STAT5A Handling References for anti-STAT5A Antibody (ABIN358237) Images back to top
Application Notes ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-STAT5A Antibody Target Details STAT5A Application Details References for anti-STAT5A Antibody (ABIN358237) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-STAT5A Antibody (ABIN358237)

Product Details anti-STAT5A Antibody Target Details STAT5A Application Details Handling Images back to top
Background publications

Martens, Uzan, Wery, Hooghe, Hooghe-Peters, Gertler: "Suppressor of cytokine signaling 7 inhibits prolactin, growth hormone, and leptin signaling by interacting with STAT5 or STAT3 and attenuating their nuclear translocation." in: The Journal of biological chemistry, Vol. 280, Issue 14, pp. 13817-23, 2005

Defilippi, Rosso, Dentelli, Calvi, Garbarino, Tarone, Pegoraro, Brizzi: "{beta}1 Integrin and IL-3R coordinately regulate STAT5 activation and anchorage-dependent proliferation." in: The Journal of cell biology, Vol. 168, Issue 7, pp. 1099-108, 2005

Sekine, Yamamoto, Yumioka, Sugiyama, Tsuji, Oritani, Shimoda, Minoguchi, Yoshimura, Matsuda: "Physical and functional interactions between STAP-2/BKS and STAT5." in: The Journal of biological chemistry, Vol. 280, Issue 9, pp. 8188-96, 2005

Sultan, Xie, LeBaron, Ealley, Nevalainen, Rui: "Stat5 promotes homotypic adhesion and inhibits invasive characteristics of human breast cancer cells." in: Oncogene, Vol. 24, Issue 5, pp. 746-60, 2005

Moriggl, Sexl, Kenner, Duntsch, Stangl, Gingras, Hoffmeyer, Bauer, Piekorz, Wang, Bunting, Wagner, Sonneck, Valent, Ihle, Beug: "Stat5 tetramer formation is associated with leukemogenesis." in: Cancer cell, Vol. 7, Issue 1, pp. 87-99, 2005

Images

Product Details anti-STAT5A Antibody Target Details STAT5A Application Details Handling References for anti-STAT5A Antibody (ABIN358237) back to top
Supplier Images
 image for anti-STAT5A antibody (Signal Transducer and Activator of Transcription 5A) (pSer726) (ABIN358237) anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (pSer726) antibody
 image for anti-STAT5A antibody (Signal Transducer and Activator of Transcription 5A) (pSer726) (ABIN358237) anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (pSer726) antibody (Image 2)