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anti-Human STAT5A antibody for Flow Cytometry

Recommended STAT5A Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 5A (STAT5A) Antibodies
  • STAT5A
  • STAT5
  • MGF
  • AA959963
  • Stat5
  • STAT5A/MGF
  • STATA5
  • signal transducer and activator of transcription 5A
  • signal transducer and activator of transcription 5A-like
  • signal transducer and activator of transcription 5B
  • STAT5A
  • Stat5a
  • LOC100348518
  • STAT5B
Epitope
AA 583-794
94
53
45
17
16
15
9
6
6
6
5
5
5
5
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
444
218
186
31
21
16
14
11
6
1
1
Host
Mouse
340
116
12
Clonality
Monoclonal
Conjugate
This STAT5A antibody is un-conjugated
12
12
10
8
6
6
5
5
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
351
222
147
77
75
69
36
31
26
18
11
9
6
4
2
2
1
1
1
1
1
1
1
Options
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Catalog No. ABIN1098129
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.102535 ABIN4898875 FACS PE Mouse IgG1 Log in to see 251610 1
13.102535 ABIN4898874 FACS Fluorescein Mouse IgG1 Log in to see 251610
13.102535 ABIN4900852 FACS ICC Mouse IgG1 Log in to see 251610
13.102535 ABIN1845625 ICC FACS IF IHC ELISA WB Mouse IgG1 AA 583-794 Log in to see
1 ABIN738276 FACS IF (p) IHC (p) WB Rabbit IgG AA 70-120 Log in to see Polyclonal 1
1 ABIN1804637 FACS IF IHC (p) WB Mouse IgG1 Log in to see 4E7
1 ABIN2666295 FACS Mouse IgG2a kappa Log in to see 9C8B50 5
1 ABIN1501193 FACS WB Mouse IgG1 Log in to see 1H9
1 ABIN1501189 FACS IF WB Mouse IgG2b Log in to see 10H1
1 ABIN1501190 FACS IF WB Mouse IgG2b Log in to see 8H1
1 ABIN1501185 FACS IF IHC (p) WB Mouse IgG2b Log in to see 6D6
1 ABIN1501186 FACS IF IHC (p) WB Mouse IgG1 Log in to see 9A5
1 ABIN1501192 FACS WB Mouse IgG1 Log in to see 3D2
1 ABIN1501191 FACS IHC (p) WB Mouse IgG2a Log in to see 11A9
1 ABIN1491603 ICC FACS IF IHC ELISA WB Mouse IgG1 Log in to see 6D4
1 ABIN340193 FACS CF Mouse IgG1 AA 774-794 Log in to see
1 ABIN4881074 ELISA FACS ICC IHC WB Mouse IgG1 Log in to see 6D4
1 ABIN4356419 FACS Alexa Fluor 405 Mouse IgG1 Log in to see 251610
1 ABIN4356418 FACS Alexa Fluor 350 Mouse IgG1 Log in to see 251610
1 ABIN4356414 FACS Alexa Fluor 700 Mouse IgG1 Log in to see 251610

Top referenced anti-STAT5A antibody for Flow Cytometry

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Application / Reactivity Human
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Cellular Assay (CA) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 1 Antibodies
Dot Blot (DB) 18 Antibodies
ELISA 135 Antibodies
Enzyme Immunoassay (EIA) 9 Antibodies
Flow Cytometry (FACS) 75 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 31 Antibodies
Immunofluorescence (IF) 66 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 213 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
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Immunoprecipitation (IP) 32 Antibodies
Immunostaining (ISt) 1 Antibodies
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General

Antigen Signal Transducer and Activator of Transcription 5A (STAT5A) Antibodies
Epitope AA 583-794
(94), (53), (45), (17), (16), (15), (9), (6), (6), (6), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(444), (218), (186), (31), (21), (16), (14), (11), (6), (1), (1)
Host Mouse
(340), (116), (12)
Clonality (Clone)
Monoclonal   ( )
Conjugate This STAT5A antibody is un-conjugated
(12), (12), (10), (8), (6), (6), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(351), (222), (147), (77), (75), (69), (36), (31), (26), (18), (11), (9), (6), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-STAT5A Antibody

Target Details STAT5A Application Details Handling References for anti-STAT5A Antibody (ABIN1098129) Images
Purification Purified
Immunogen Purified recombinant fragment of human STAT5A (AA 583-794 ) expressed in E. coli.
Clone 6D4
Isotype IgG1

Target Details STAT5A

Product Details anti-STAT5A Antibody Application Details Handling References for anti-STAT5A Antibody (ABIN1098129) Images back to top
Antigen
Alternative Name STAT5A (STAT5A Antibody Abstract)
Background The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. , ,
Molecular Weight 90.6 kDa
Gene ID 6776
HGNC 6776
Pathways JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus

Application Details

Product Details anti-STAT5A Antibody Target Details STAT5A Handling References for anti-STAT5A Antibody (ABIN1098129) Images back to top
Application Notes Recommended Dilution:
ELISA: Propose dilution 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/50, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-STAT5A Antibody Target Details STAT5A Application Details References for anti-STAT5A Antibody (ABIN1098129) Images back to top
Format Liquid
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-STAT5A Antibody (ABIN1098129)

Product Details anti-STAT5A Antibody Target Details STAT5A Application Details Handling Images back to top
Product cited in:

Cerliani, Guillardoy, Giulianelli, Vaque, Gutkind, Vanzulli, Martins, Zeitlin, Lamb, Lanari: "Interaction between FGFR-2, STAT5, and progesterone receptors in breast cancer." in: Cancer research, Vol. 71, Issue 10, pp. 3720-31, 2011

Warsch, Kollmann, Eckelhart, Fajmann, Cerny-Reiterer, Hölbl, Gleixner, Dworzak, Mayerhofer, Hoermann, Herrmann, Sillaber, Egger, Valent, Moriggl, Sexl: "High STAT5 levels mediate imatinib resistance and indicate disease progression in chronic myeloid leukemia." in: Blood, Vol. 117, Issue 12, pp. 3409-20, 2011

Images

Product Details anti-STAT5A Antibody Target Details STAT5A Application Details Handling References for anti-STAT5A Antibody (ABIN1098129) back to top
Supplier Images
ELISA image for anti-STAT5A antibody (Signal Transducer and Activator of Transcription 5A) (AA 583-794) (ABIN1098129) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-STAT5A antibody (Signal Transducer and Activator of Transcription 5A) (AA 583-794) (ABIN1098129) Figure 2: Flow cytometric analysis of K562 cells using STAT5A mouse mAb (green) and n...
Immunocytochemistry (ICC) image for anti-STAT5A antibody (Signal Transducer and Activator of Transcription 5A) (AA 583-794) (ABIN1098129) Figure 3: Immunofluorescence analysis of Hela cells using STAT5A mouse mAb (green) . ...
Immunohistochemistry (IHC) image for anti-STAT5A antibody (Signal Transducer and Activator of Transcription 5A) (AA 583-794) (ABIN1098129) Figure 4: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues us...
Immunohistochemistry (IHC) image for anti-STAT5A antibody (Signal Transducer and Activator of Transcription 5A) (AA 583-794) (ABIN1098129) Figure 5: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues usi...
Western Blotting (WB) image for anti-STAT5A antibody (Signal Transducer and Activator of Transcription 5A) (AA 583-794) (ABIN1098129) Figure 6: Western blot analysis using STAT5A mouse mAb against K562 (1), MOLT4 (2), H...
Western Blotting (WB) image for anti-STAT5A antibody (Signal Transducer and Activator of Transcription 5A) (AA 583-794) (ABIN1098129) Figure 7: Western blot analysis using STAT5A mAb against human STAT5A recombinant pro...