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anti-Human STAT5A antibody for Intracellular Staining

Recommended STAT5A Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 5A (STAT5A) Antibodies
  • AA959963
  • MGF
  • STAT5
  • Stat5
  • STAT5A
  • STAT5A/MGF
  • STATA5
Epitope
pTyr694
85
52
42
17
15
13
9
6
6
6
5
5
5
4
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
405
195
157
28
21
16
13
11
6
1
Host
Mouse
322
96
10
Clonality
Monoclonal
Conjugate
This STAT5A antibody is un-conjugated
12
11
10
8
6
5
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Intracellular Staining (ICS), Western Blotting (WB)
323
195
132
55
55
51
33
22
18
16
12
9
6
4
4
2
2
1
1
1
1
1
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Catalog No. ABIN968701
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008676689 ABIN1177247 ICS PE-Cy7 Mouse IgG1, kappa pTyr694 Log in to see 47-Stat5 4
0.0008676689 ABIN1177251 ICS Pacific Blue Mouse IgG1, kappa pTyr694 Log in to see 47-Stat5 4
0.0008676689 ABIN1177249 ICS Alexa Fluor 647 Mouse IgG1, kappa pTyr694 Log in to see 47-Stat5 4
0.0008676689 ABIN1177250 ICS Alexa Fluor 488 Mouse IgG1, kappa pTyr694 Log in to see 47-Stat5 4
0.0008676689 ABIN1177254 ICS Alexa Fluor 488 Mouse IgG1, kappa pTyr694 Log in to see 47-Stat5 8
0.0008676689 ABIN1177255 ICS PE-CF594 Mouse IgG1, kappa pTyr694 Log in to see 47-Stat5 4
0.0008676689 ABIN1177246 ICS PerCP-Cy5.5 Mouse IgG1, kappa pTyr694 Log in to see 47-Stat5 9
0.0008676689 ABIN1177248 ICS PE Mouse IgG1, kappa pTyr694 Log in to see 47-Stat5 4
0.0008676689 ABIN1177252 ICS PE Mouse IgG1, kappa pTyr694 Log in to see 47-Stat5
0.0008676689 ABIN1177253 ICS Alexa Fluor 647 Mouse IgG1, kappa pTyr694 Log in to see 47-Stat5
0.0008676689 ABIN2680989 FACS ICS FITC Mouse IgG1 kappa pTyr694 Log in to see SRBCZX
0.0008676689 ABIN2680993 FACS ICS APC Mouse IgG1 kappa pTyr694 Log in to see SRBCZX
0.0008676689 ABIN2680995 FACS ICS PE-Cy7 Mouse IgG1 kappa pTyr694 Log in to see SRBCZX
0.0008676689 ABIN2680991 FACS ICS PE Mouse IgG1 kappa pTyr694 Log in to see SRBCZX
0.0008676689 ABIN2680997 FACS ICS PerCP-eFluor® 710 Mouse IgG1 kappa pTyr694 Log in to see SRBCZX
0.0008676689 ABIN2680999 FACS ICS PE-eFluor® 610 Mouse IgG1 kappa pTyr694 Log in to see SRBCZX

Top referenced anti-STAT5A antibody for Intracellular Staining

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Application / Reactivity Human
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ELISA 119 Antibodies
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Flow Cytometry (FACS) 54 Antibodies
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General

Antigen Signal Transducer and Activator of Transcription 5A (STAT5A) Antibodies
Epitope pTyr694
(85), (52), (42), (17), (15), (13), (9), (6), (6), (6), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(405), (195), (157), (28), (21), (16), (13), (11), (6), (1)
Host Mouse
(322), (96), (10)
Clonality (Clone)
Monoclonal   ( )
Conjugate This STAT5A antibody is un-conjugated
(12), (11), (10), (8), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Intracellular Staining (ICS), Western Blotting (WB)
(323), (195), (132), (55), (55), (51), (33), (22), (18), (16), (12), (9), (6), (4), (4), (2), (2), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-STAT5A Antibody

Target Details STAT5A Application Details Handling References for anti-STAT5A Antibody (ABIN968701) Images
Cross-Reactivity Human
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Sheep Stat5 Peptide
Clone 47-Stat5
Isotype IgG1, kappa

Target Details STAT5A

Product Details anti-STAT5A Antibody Application Details Handling References for anti-STAT5A Antibody (ABIN968701) Images back to top
Antigen
Alternative Name Stat5 (STAT5A Antibody Abstract)
Background The Stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Seven mammalian STATs have been identified: Stat 1-4, Stat5a, 5b, and Stat6. The SH3 domain of Stat5 shows extensive homology with the SH2 domains of Stat1 and Stat2. This homology leads to speculation that it may have a broader physiological role than originally anticipated and has led to its classification as a member of the Stat family. Stat5 has been further characterized and shown to be encoded by two separate genes, Stat5a and Stat5b that share over 90% identity at the amino acid level. Further insight into the exact biological role of Stat5a and Stat5b was determined with the generation of Stat5a and Stat5b deficient mice. Stat5a was shown to be involved in lactogenesis and mammary development, while Stat5b was shown to be involved in growth hormone signaling and to play a role in liver gene expression. Both Stat5a and Stat5b share similarities, both are involved in IL-2 induced peripheral T cell proliferation. Stat5 (MGF) mRNA expression is highest in mammary gland tissue. Lower levels are found in ovary, thymus, spleen, kidney, lung, muscle, and the adrenal gland. The peptide hormone, prolactin, binds to the prolactin receptor (PRLR) to initiate the lactogenic response. There are at least three forms of PRLR, however, only the long form is able to activate the 92 kDa Stat5 protein by inducing phosphorylation at Tyr694. Once phosphorylated, Stat5 becomes an essential transcription factor which binds to the beta-casein gene promoter. Stat5 activity is tightly regulated throughout gestation, lactation, and post-lactation. Treatment of activated Stat5 with a protein tyrosine phosphatase results in the loss of DNA binding activity. The presence of an SH2 domain within Stat5 suggests that it may directly interact with protein tyrosine kinases (PTKs) such as JAK2. This antibody is routinely tested by Western blot analysis.
Molecular Weight 92 kDa
Research Area Signaling, Receptors, Inflammation
Pathways JAK-STAT Signaling, RTK Signaling

Application Details

Product Details anti-STAT5A Antibody Target Details STAT5A Handling References for anti-STAT5A Antibody (ABIN968701) Images back to top
Application Notes For use in flow cytometry the conjugated formats of Anti-Human Stat5 (pY694) are recommended (PE-labeled, Alexa Fluor 488® and Alexa Fluor 647®).
Comment

Related Products: ABIN968534

Restrictions For Research Use only

Handling

Product Details anti-STAT5A Antibody Target Details STAT5A Application Details References for anti-STAT5A Antibody (ABIN968701) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-STAT5A Antibody (ABIN968701)

Product Details anti-STAT5A Antibody Target Details STAT5A Application Details Handling Images back to top
Product cited in:

Williams, Cheung, Park, Razani, Cohen, Muller, Di Vizio, Chopra, Pestell, Lisanti: "Loss of caveolin-1 gene expression accelerates the development of dysplastic mammary lesions in tumor-prone transgenic mice." in: Molecular biology of the cell, Vol. 14, Issue 3, pp. 1027-42, 2003

Park, Lee, Frank, Razani, Nguyen, Parlow, Russell, Hulit, Pestell, Lisanti: "Caveolin-1-deficient mice show accelerated mammary gland development during pregnancy, premature lactation, and hyperactivation of the Jak-2/STAT5a signaling cascade." in: Molecular biology of the cell, Vol. 13, Issue 10, pp. 3416-30, 2002

Gouilleux, Pallard, Dusanter-Fourt, Wakao, Haldosen, Norstedt, Levy, Groner: "Prolactin, growth hormone, erythropoietin and granulocyte-macrophage colony stimulating factor induce MGF-Stat5 DNA binding activity." in: The EMBO journal, Vol. 14, Issue 9, pp. 2005-13, 1995

Gouilleux, Wakao, Mundt, Groner: "Prolactin induces phosphorylation of Tyr694 of Stat5 (MGF), a prerequisite for DNA binding and induction of transcription." in: The EMBO journal, Vol. 13, Issue 18, pp. 4361-9, 1994

Wakao, Gouilleux, Groner: "Mammary gland factor (MGF) is a novel member of the cytokine regulated transcription factor gene family and confers the prolactin response." in: The EMBO journal, Vol. 13, Issue 9, pp. 2182-91, 1994

Images

Product Details anti-STAT5A Antibody Target Details STAT5A Application Details Handling References for anti-STAT5A Antibody (ABIN968701) back to top
Supplier Images
 image for anti-STAT5A antibody (Signal Transducer and Activator of Transcription 5A) (pTyr694) (ABIN968701) A431 cells were either left untreated (-) or treated (+) with 100 ng/ml EGF for 5 min...