anti-Human STAT5A antibody for Immunofluorescence

Recommended STAT5A Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 5A (STAT5A) Antibodies
  • STAT5A
  • STAT5
  • MGF
  • AA959963
  • Stat5
  • STAT5A/MGF
  • STATA5
  • signal transducer and activator of transcription 5A
  • signal transducer and activator of transcription 5A-like
  • signal transducer and activator of transcription 5B
  • STAT5A
  • Stat5a
  • LOC100348518
  • STAT5B
Epitope
AA 451-649
102
56
47
22
19
16
9
8
6
6
5
5
5
5
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
502
230
198
35
24
19
17
16
7
4
4
1
1
1
1
1
1
Host
Mouse
359
149
14
Clonality
Monoclonal
Conjugate
This STAT5A antibody is un-conjugated
13
13
11
9
9
9
5
5
5
5
5
5
5
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
397
233
163
96
95
88
47
43
26
17
11
9
7
7
6
4
3
2
2
1
1
1
1
1
1
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Catalog No. ABIN967811
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.14905 ABIN967810 IF IP IHC WB Mouse IgG2b AA 451-649 Log in to see 89-Stat5 5
12.14905 ABIN968670 IF WB Rabbit Ig pTyr694 Log in to see Polyclonal 3
12.14905 ABIN968669 IF WB Rabbit Ig pTyr694 Log in to see Polyclonal 3
12.14905 ABIN2705266 IC IF IP IHC WB Rabbit C-Term, pSer780 Log in to see Polyclonal
12.14905 ABIN2707112 IC IF WB Rabbit Center Log in to see Polyclonal
12.14905 ABIN520540 IF WB Mouse AA 1-794, full length Log in to see Polyclonal
12.14905 ABIN950127 IF WB Mouse AA 1-794, full length Log in to see Polyclonal
12.14905 ABIN4356401 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN389668 IF IHC (p) WB Rabbit Ig pTyr694 Log in to see Polyclonal 1
1 ABIN5550843 EIA IF IHC (p) WB Mouse IgG1 Tyr694 Log in to see 5F6-F1
1 ABIN3187089 ELISA IF WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN126900 EIA IHC (fro) IF WB Mouse IgG1 pTyr695, pTyr699 Log in to see 5G4
1 ABIN1104496 ELISA IF IHC (p) WB Mouse IgG pTyr694 Log in to see 5F6-F1
1 ABIN1804637 FACS IF IHC (p) WB Mouse IgG1 Log in to see 4E7
1 ABIN1501189 FACS IF WB Mouse IgG2b Log in to see 10H1
1 ABIN1501186 FACS IF IHC (p) WB Mouse IgG1 Log in to see 9A5
1 ABIN1501185 FACS IF IHC (p) WB Mouse IgG2b Log in to see 6D6
1 ABIN1501190 FACS IF WB Mouse IgG2b Log in to see 8H1
1 ABIN272189 IF WB Rabbit Log in to see Polyclonal
1 ABIN1491603 ELISA FACS ICC IF IHC (p) WB Mouse IgG1 Log in to see 6D4

General

Antigen Signal Transducer and Activator of Transcription 5A (STAT5A) Antibodies
Epitope AA 451-649
(102), (56), (47), (22), (19), (16), (9), (8), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(502), (230), (198), (35), (24), (19), (17), (16), (7), (4), (4), (1), (1), (1), (1), (1), (1)
Host Mouse
(359), (149), (14)
Clonality (Clone)
Monoclonal   ( )
Conjugate This STAT5A antibody is un-conjugated
(13), (13), (11), (9), (9), (9), (5), (5), (5), (5), (5), (5), (5), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(397), (233), (163), (96), (95), (88), (47), (43), (26), (17), (11), (9), (7), (7), (6), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-STAT5A Antibody

Target Details STAT5A Application Details Handling References for anti-STAT5A antibody (ABIN967811) Images
Cross-Reactivity Human, Dog (Canine), Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Sheep Stat5 aa. 451-649
Clone 89-Stat5
Isotype IgG2b

Target Details STAT5A

Product Details anti-STAT5A Antibody Application Details Handling References for anti-STAT5A antibody (ABIN967811) Images back to top
Antigen
Alternative Name Stat5 (STAT5A Antibody Abstract)
Background Stat5, also known as mammary gland factor (MGF) has been shown to play a critical role in the lactogenic hormone response and has been well characterized in mammary epithelial cells. The SH3 domain of MGF shows extensive homology with the SH2 domains of Stat1 and Stat2. This homology has led to its classification as a member of the Stat family. The Stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment and activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Stat5a has been shown to be involved in lactogenesis and mammary development, while Stat5b has been shown to be involved in growth hormone signaling and to play a role in liver gene expression. Stat5 activity is tightly regulated throughout gestation, lactation, and post-lactation.
Synonyms: MGF
Molecular Weight 92 kDa
Research Area Signaling, Receptors, Inflammation
Pathways JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation

Application Details

Product Details anti-STAT5A Antibody Target Details STAT5A Handling References for anti-STAT5A antibody (ABIN967811) Images back to top
Comment

Related Products: ABIN968536, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-STAT5A Antibody Target Details STAT5A Application Details References for anti-STAT5A antibody (ABIN967811) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-STAT5A antibody (ABIN967811)

Product Details anti-STAT5A Antibody Target Details STAT5A Application Details Handling Images back to top
Product cited in:

Buitenhuis, Baltus, Lammers, Coffer, Koenderman: "Signal transducer and activator of transcription 5a (STAT5a) is required for eosinophil differentiation of human cord blood-derived CD34+ cells." in: Blood, Vol. 101, Issue 1, pp. 134-42, 2002

Chughtai, Schimchowitsch, Lebrun, Ali: "Prolactin induces SHP-2 association with Stat5, nuclear translocation, and binding to the beta-casein gene promoter in mammary cells." in: The Journal of biological chemistry, Vol. 277, Issue 34, pp. 31107-14, 2002

Sadowski, Choi, Le, Wheeler, Wang, Sadowski: "Insulin Induction of SOCS-2 and SOCS-3 mRNA expression in C2C12 Skeletal Muscle Cells Is Mediated by Stat5*." in: The Journal of biological chemistry, Vol. 276, Issue 23, pp. 20703-10, 2001

Kirken, Malabarba, Xu, Liu, Farrar, Hennighausen, Larner, Grimley, Rui: "Prolactin stimulates serine/tyrosine phosphorylation and formation of heterocomplexes of multiple Stat5 isoforms in Nb2 lymphocytes." in: The Journal of biological chemistry, Vol. 272, Issue 22, pp. 14098-103, 1997

Gouilleux, Wakao, Mundt, Groner: "Prolactin induces phosphorylation of Tyr694 of Stat5 (MGF), a prerequisite for DNA binding and induction of transcription." in: The EMBO journal, Vol. 13, Issue 18, pp. 4361-9, 1994

Images

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Supplier Images
Western Blotting (WB) image for anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (AA 451-649) antibody (ABIN967811) Western blot analysis of Stat5 on human endothelial cell lysate. Lane 1: 1:250, lane ...
Immunofluorescence (IF) image for anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (AA 451-649) antibody (ABIN967811) Immunofluorescent staining of rabbit muscle with anti-Stat5 antibody.
 image for anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (AA 451-649) antibody (ABIN967811) anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (AA 451-649) antibody (Image 3)
Western Blotting (WB) image for anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (AA 451-649) antibody (ABIN967811) anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (AA 451-649) antibody (Image 4)