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anti-Mouse (Murine) STAT5A antibody for Enzyme Immunoassay

Recommended STAT5A Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 5A (STAT5A) Antibodies
  • STAT5A
  • STAT5
  • MGF
  • AA959963
  • Stat5
  • STAT5A/MGF
  • STATA5
  • signal transducer and activator of transcription 5A
  • signal transducer and activator of transcription 5A-like
  • signal transducer and activator of transcription 5B
  • STAT5A
  • Stat5a
  • LOC100348518
  • STAT5B
Epitope
Tyr694
94
53
45
17
16
14
9
6
6
6
5
5
5
5
4
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
443
217
185
31
21
16
14
11
6
1
1
Host
Rabbit
338
117
12
Clonality
Polyclonal
Conjugate
This STAT5A antibody is un-conjugated
12
12
10
8
6
6
5
5
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
350
223
148
76
76
69
35
32
26
18
11
8
6
4
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN401413
$ 522.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN400812 EIA IF IHC (p) WB Mouse IgG1 Tyr694 Log in to see 5F6-F1 4
1 ABIN271829 EIA IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN401412 EIA WB Rabbit IgG C-Term Log in to see Polyclonal 1
1 ABIN372786 EIA IHC (p) WB Rabbit IgG Tyr694 Log in to see Polyclonal

Top referenced anti-STAT5A antibody for Enzyme Immunoassay

Similar anti-STAT5A Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 51 Antibodies 64 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies 5 Antibodies
Flow Cytometry (FACS) 19 Antibodies 17 Antibodies
Immunochromatography (IC) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 16 Antibodies 17 Antibodies
Immunofluorescence (IF) 27 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 105 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 45 Antibodies
Immunoprecipitation (IP) 13 Antibodies
Intracellular Staining (ICS) 9 Antibodies
Western Blotting (WB) 136 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Gel Shift (GS) 1 Antibodies

General

Antigen Signal Transducer and Activator of Transcription 5A (STAT5A) Antibodies
Epitope Tyr694
(94), (53), (45), (17), (16), (14), (9), (6), (6), (6), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(443), (217), (185), (31), (21), (16), (14), (11), (6), (1), (1)
Host Rabbit
(338), (117), (12)
Clonality
Polyclonal
Conjugate This STAT5A antibody is un-conjugated
(12), (12), (10), (8), (6), (6), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(350), (223), (148), (76), (76), (69), (35), (32), (26), (18), (11), (8), (6), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-STAT5A Antibody

Target Details STAT5A Application Details Handling References for anti-STAT5A Antibody (ABIN401413) Images
Specificity This antibody is specific for Stat5alpha protein phosphorylated at Y694.
Cross-Reactivity (Details) Species reactivity (expected):Rat, Human.
Species reactivity (tested):Mouse.
Purification Affinity chromatography
Immunogen synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5alpha protein
Isotype IgG

Target Details STAT5A

Product Details anti-STAT5A Antibody Application Details Handling References for anti-STAT5A Antibody (ABIN401413) Images back to top
Antigen
Alternative Name STAT5 / STAT5A (STAT5A Antibody Abstract)
Background Signal trans-ducer and activator of transcription 5 (Stat5) belongs to a family of cytoplasmic trans-cription factors that can be activated (phosphorylated) by a cell surface receptor. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Stat5 has two isoforms, Stat5alpha and Stat5beta. Aberrant Stat5 activation has been implicated in the pathogenesis of chronic myelogenous leukemia, prostate and breast cancer and tumor metastasis. Stat5 is localized in the cytoplasm and upon phosphorylation at Y694 is translocated to the nucleus.Synonyms: MGF, Mammary gland factor, STAT-5, STAT-5A, Signal transducer and activator of transcription 5A
Gene ID 20850
NCBI Accession NP_035618
UniProt P42230
Pathways JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process

Application Details

Product Details anti-STAT5A Antibody Target Details STAT5A Handling References for anti-STAT5A Antibody (ABIN401413) Images back to top
Application Notes ELISA: 1/25,000. Western Blot: 1/500 - 1/2,000. Immunohistochemistry: 20 - 40 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-STAT5A Antibody Target Details STAT5A Application Details References for anti-STAT5A Antibody (ABIN401413) Images back to top
Concentration 1.1 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C

References for anti-STAT5A Antibody (ABIN401413)

Product Details anti-STAT5A Antibody Target Details STAT5A Application Details Handling Images back to top
Background publications

Baugh, Floyd, Stephens: "The modulation of STAT5A/GR complexes during fat cell differentiation and in mature adipocytes." in: Obesity (Silver Spring, Md.), Vol. 15, Issue 3, pp. 583-90, 2007

Laurence, Tato, Davidson, Kanno, Chen, Yao, Blank, Meylan, Siegel, Hennighausen, Shevach, Oshea: "Interleukin-2 signaling via STAT5 constrains T helper 17 cell generation." in: Immunity, Vol. 26, Issue 3, pp. 371-81, 2007

Engblom, Kornfeld, Schwake, Tronche, Reimann, Beug, Hennighausen, Moriggl, Schütz: "Direct glucocorticoid receptor-Stat5 interaction in hepatocytes controls body size and maturation-related gene expression." in: Genes & development, Vol. 21, Issue 10, pp. 1157-62, 2007

Burchill, Yang, Vogtenhuber, Blazar, Farrar: "IL-2 receptor beta-dependent STAT5 activation is required for the development of Foxp3+ regulatory T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 1, pp. 280-90, 2006

Images

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Supplier Images
 image for anti-STAT5A antibody (Signal Transducer and Activator of Transcription 5A) (Tyr694) (ABIN401413) anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (Tyr694) antibody
 image for anti-STAT5A antibody (Signal Transducer and Activator of Transcription 5A) (Tyr694) (ABIN401413) anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (Tyr694) antibody (Image 2)