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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||50 Antibodies||61 Antibodies|
|Enzyme Immunoassay (EIA)||5 Antibodies||5 Antibodies|
|Flow Cytometry (FACS)||7 Antibodies||4 Antibodies|
|Gel Shift (GS)||1 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||18 Antibodies|
|Immunofluorescence (IF)||31 Antibodies|
|Immunofluorescence (Cultured Cells) (IF (cc))||1 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||26 Antibodies|
|Immunohistochemistry (IHC)||100 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||57 Antibodies|
|Immunoprecipitation (IP)||17 Antibodies|
|Intracellular Staining (ICS)||9 Antibodies|
|Western Blotting (WB)||149 Antibodies|
|Fluorescence Microscopy (FM)||1 Antibodies|
|Antigen||Signal Transducer and Activator of Transcription 5A (STAT5A) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This STAT5A antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-STAT5A AntibodyTarget Details STAT5A Application Details Handling Images
|Specificity||This antibody detects endogenous levels of STAT5A protein. (region surrounding Ala688)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen|
|Purity||> 95 % pure by SDS-PAGE|
Target Details STAT5AProduct Details anti-STAT5A Antibody Application Details Handling Images back to top
|Alternative Name||STAT5 / STAT5A (STAT5A Antibody Abstract)|
|Background||Stat 5a protein is a transcription factor activated by hormone and cytokines. Two highly related, but distinct Stat 5 genes (Stat 5a and Stat 5b) were identified in mouse. The amino acid sequences of Stat 5a and Stat 5b show 96 % sequence similarity, and both proteins are co expressed in most tissues of both virgin and lactating mice. However, differential accumulation of Stat 5a and Stat 5b mRNA has been reported for both muscle and mammary tissue. Stat 5a is critically involved in a variety of physiological functions, including reproduction, lactation, immune function and somatic growth.Synonyms: MGF, Mammary gland factor, STAT-5, STAT-5A, Signal transducer and activator of transcription 5A|
|Molecular Weight||approx. 90 kDa|
|Pathways||JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation|
Application DetailsProduct Details anti-STAT5A Antibody Target Details STAT5A Handling Images back to top
ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-STAT5A Antibody Target Details STAT5A Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||DO NOT FREEZE!|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|
ImagesProduct Details anti-STAT5A Antibody Target Details STAT5A Application Details Handling back to top