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anti-Mouse (Murine) STAT5A antibody for Flow Cytometry

Recommended STAT5A Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 5A (STAT5A) Antibodies
  • AA959963
  • MGF
  • STAT5
  • Stat5
  • STAT5A
  • STAT5A/MGF
  • STATA5
Epitope
pTyr694
85
52
42
17
15
13
9
6
6
6
5
5
5
4
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
405
194
157
28
21
16
13
11
6
1
Host
Mouse
322
96
10
Clonality
Monoclonal
Conjugate
This STAT5A antibody is conjugated to FITC
12
10
10
8
6
5
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Intracellular Staining (ICS)
324
195
132
55
54
51
33
22
18
16
12
9
6
4
4
2
2
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN2680989
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3068567 ABIN2680993 FACS ICS APC Mouse IgG1 kappa pTyr694 Log in to see SRBCZX
0.3068567 ABIN2680995 FACS ICS PE-Cy7 Mouse IgG1 kappa pTyr694 Log in to see SRBCZX
0.3068567 ABIN2680991 FACS ICS PE Mouse IgG1 kappa pTyr694 Log in to see SRBCZX
0.3068567 ABIN2680997 FACS ICS PerCP-eFluor® 710 Mouse IgG1 kappa pTyr694 Log in to see SRBCZX
0.3068567 ABIN2680999 FACS ICS PE-eFluor® 610 Mouse IgG1 kappa pTyr694 Log in to see SRBCZX
0.0010666476 ABIN2666295 FACS Mouse IgG2a kappa Log in to see 9C8B50 5
0.0010666476 ABIN2367489 FACS ELISA WB Rabbit IgG pTyr694 Log in to see Polyclonal
0.0010666476 ABIN3060440 FACS IP IHC WB Rabbit Log in to see Polyclonal

Top referenced anti-STAT5A antibody for Flow Cytometry

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General

Antigen Signal Transducer and Activator of Transcription 5A (STAT5A) Antibodies
Epitope pTyr694
(85), (52), (42), (17), (15), (13), (9), (6), (6), (6), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(405), (194), (157), (28), (21), (16), (13), (11), (6), (1)
Host Mouse
(322), (96), (10)
Clonality (Clone)
Monoclonal   ( )
Conjugate This STAT5A antibody is conjugated to FITC
(12), (10), (10), (8), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Intracellular Staining (ICS)
(324), (195), (132), (55), (54), (51), (33), (22), (18), (16), (12), (9), (6), (4), (4), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-STAT5A Antibody

Target Details STAT5A Application Details Handling Images
Specificity This SRBCZX monoclonal antibody recognizes signal transducer and activator of transcription 5 (STAT5) when phosphorylated on tyrosine 694. STAT proteins are activated by ligand binding to receptors, such as cytokine receptors, that associate with Janus kinase (JAK) family members. Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. In response to cytokines that signal through the common gamma chain such as IL-2, IL-7, and IL-15, STAT5 is phosphorylated on tyrosine 694 by JAK1 and JAK3. Cytokines such as IL-3, IL-5, and GM-CSF that signal via the common beta chain induce STAT5 phosphorylation on tyrosine 694 by JAK 2. Phosphorylation of STAT5 on tyrosine 694 is essential for STAT5 dimer formation, nuclear translocation, and DNA binding activity.

Specificity of this SRBCZX clone was determined by ELISA and flow cytometry.
Characteristics This SRBCZX monoclonal antibody recognizes signal transducer and activator of transcription 5 (STAT5) when phosphorylated on tyrosine 694. STAT proteins are activated by ligand binding to receptors, such as cytokine receptors, that associate with Janus kinase (JAK) family members. Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. In response to cytokines that signal through the common gamma chain such as IL-2, IL-7, and IL-15, STAT5 is phosphorylated on tyrosine 694 by JAK1 and JAK3. Cytokines such as IL-3, IL-5, and GM- CSF that signal via the common beta chain induce STAT5 phosphorylation on tyrosine 694 by JAK 2. Phosphorylation of STAT5 on tyrosine 694 is essential for STAT5 dimer formation, nuclear translocation, and DNA binding activity. Specificity of this SRBCZX clone was determined by ELISA and flow cytometry.
Synonyms: Synonyms: signal transducer and activator of transcription 5, pSTAT5
Clone SRBCZX
Isotype IgG1 kappa

Target Details STAT5A

Product Details anti-STAT5A Antibody Application Details Handling Images back to top
Antigen
Alternative Name STAT5 (STAT5A Antibody Abstract)
Research Area Cancer, Transcription Factors, Signaling, Receptors, Inflammation
Pathways JAK-STAT Signaling, RTK Signaling

Application Details

Product Details anti-STAT5A Antibody Target Details STAT5A Handling Images back to top
Application Notes Applications reported: This SRBCZX antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications tested: This SRBCZX antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated U937 cells. This can be used at 5 μL (1 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10 5 to 10 8 cells/test. Staining Protocol: We recommend using Protocol C: Two-step protocol: Fixation/Methanol. Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins and Protocol B: One-step protocol: intracellular (nuclear) proteins cannot be used. All Protocols can be found in the Flow Cytometry Protocols: "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the Best Protocols Section under the Resources tab online.
Restrictions For Research Use only

Handling

Product Details anti-STAT5A Antibody Target Details STAT5A Application Details Images back to top
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Handling Advice Do not freeze. Light-sensitive material.
Storage 4 °C
Storage Comment Store at 2-8°C.

Images

Product Details anti-STAT5A Antibody Target Details STAT5A Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-STAT5A antibody (Signal Transducer and Activator of Transcription 5A) (pTyr694) (FITC) (ABIN2680989) anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (pTyr694) antibody (FITC)