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anti-Mouse (Murine) STAT5A antibody for Intracellular Staining

Recommended STAT5A Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 5A (STAT5A) Antibodies
  • AA959963
  • MGF
  • STAT5
  • Stat5
  • STAT5A
  • STAT5A/MGF
  • STATA5
Epitope
pTyr694
85
52
42
17
15
13
9
6
6
6
5
5
5
4
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)
405
194
156
28
20
16
13
11
6
1
Host
Mouse
322
96
10
Clonality
Monoclonal
Conjugate
This STAT5A antibody is conjugated to PE-Cy7
12
11
10
8
6
5
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Intracellular Staining (ICS)
324
195
132
55
55
51
33
22
18
16
12
9
6
4
4
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN1177247
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008708181 ABIN1177251 ICS Pacific Blue Mouse IgG1, kappa pTyr694 Log in to see 47-Stat5 4
0.0008708181 ABIN1177249 ICS Alexa Fluor 647 Mouse IgG1, kappa pTyr694 Log in to see 47-Stat5 4
0.0008708181 ABIN1177250 ICS Alexa Fluor 488 Mouse IgG1, kappa pTyr694 Log in to see 47-Stat5 4
0.0008708181 ABIN1177254 ICS Alexa Fluor 488 Mouse IgG1, kappa pTyr694 Log in to see 47-Stat5 8
0.0008708181 ABIN1177246 ICS PerCP-Cy5.5 Mouse IgG1, kappa pTyr694 Log in to see 47-Stat5 9
0.0008708181 ABIN1177248 ICS PE Mouse IgG1, kappa pTyr694 Log in to see 47-Stat5 4
0.0008708181 ABIN1177252 ICS PE Mouse IgG1, kappa pTyr694 Log in to see 47-Stat5
0.0008708181 ABIN1177253 ICS Alexa Fluor 647 Mouse IgG1, kappa pTyr694 Log in to see 47-Stat5
0.0008708181 ABIN2680989 FACS ICS FITC Mouse IgG1 kappa pTyr694 Log in to see SRBCZX
0.0008708181 ABIN2680993 FACS ICS APC Mouse IgG1 kappa pTyr694 Log in to see SRBCZX
0.0008708181 ABIN2680995 FACS ICS PE-Cy7 Mouse IgG1 kappa pTyr694 Log in to see SRBCZX
0.0008708181 ABIN2680991 FACS ICS PE Mouse IgG1 kappa pTyr694 Log in to see SRBCZX
0.0008708181 ABIN2680997 FACS ICS PerCP-eFluor® 710 Mouse IgG1 kappa pTyr694 Log in to see SRBCZX
0.0008708181 ABIN2680999 FACS ICS PE-eFluor® 610 Mouse IgG1 kappa pTyr694 Log in to see SRBCZX

Top referenced anti-STAT5A antibody for Intracellular Staining

Similar anti-STAT5A Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Sheep (Ovine)
Western Blotting (WB) 303 Antibodies 143 Antibodies 113 Antibodies 10 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Luminex Assay (LMNX) 6 Antibodies
Intracellular Staining (ICS) 17 Antibodies 15 Antibodies 9 Antibodies 9 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 29 Antibodies 17 Antibodies 12 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 55 Antibodies 26 Antibodies 23 Antibodies
Immunohistochemistry (IHC) 186 Antibodies 98 Antibodies

General

Antigen Signal Transducer and Activator of Transcription 5A (STAT5A) Antibodies
Epitope pTyr694
(85), (52), (42), (17), (15), (13), (9), (6), (6), (6), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)
(405), (194), (156), (28), (20), (16), (13), (11), (6), (1)
Host Mouse
(322), (96), (10)
Clonality (Clone)
Monoclonal   ( )
Conjugate This STAT5A antibody is conjugated to PE-Cy7
(12), (11), (10), (8), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Intracellular Staining (ICS)
(324), (195), (132), (55), (55), (51), (33), (22), (18), (16), (12), (9), (6), (4), (4), (2), (2), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-STAT5A Antibody

Target Details STAT5A Application Details Handling References for anti-STAT5A Antibody (ABIN1177247) Images
Brand BD Phosflow™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Sheep Stat5 Peptide
Clone 47-Stat5
Isotype IgG1, kappa

Target Details STAT5A

Product Details anti-STAT5A Antibody Application Details Handling References for anti-STAT5A Antibody (ABIN1177247) Images back to top
Antigen
Alternative Name Stat5 (STAT5A Antibody Abstract)
Background Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat5 has been characterized and shown to be encoded by two separate genes, Stat5a and Stat5b that share over 90% identity at the amino acid level. Stat5a has been shown to be involved in lactogenesis and mammary development, while Stat5b has been shown to be involved in growth hormone signaling and to play a role in liver gene expression. Both Stat5a and Stat5b share similarities, both are involved in IL-2 induced peripheral T cell proliferation. The peptide hormone, prolactin, binds to the prolactin receptor (PRLR) to initiate the lactogenic response. There are at least three forms of PRLR, however, only the long form is able to activate the 92-kDa Stat5 protein by inducing phosphorylation at Y694. Once phosphorylated, Stat5 becomes an essential transcription factor which binds to the beta-casein gene promoter. The presence of an SH2 domain within Stat5 suggests that it may directly interact with protein tyrosine kinases (PTKs) such as JAK2. The 47 monoclonal antibody recognizes the phosphorylated Y694 of Stat5a. The homologous phosphorylation site in Stat5b is Y699.
Research Area Inflammation
Pathways JAK-STAT Signaling, RTK Signaling

Application Details

Product Details anti-STAT5A Antibody Target Details STAT5A Handling References for anti-STAT5A Antibody (ABIN1177247) Images back to top
Application Notes This antibody conjugate is suitable for intracellular staining of human whole blood (using BD™ Phosflow Lyse/Fix Buffer) and peripheral blood mononuclear cells (using BD Cytofix™ Fixation Buffer or BD™ Phosflow Fix Buffer I).
Sample Volume 20 μL
Restrictions For Research Use only

Handling

Product Details anti-STAT5A Antibody Target Details STAT5A Application Details References for anti-STAT5A Antibody (ABIN1177247) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment The antibody was conjugated with PE-Cy7 under optimum conditions, and unconjugated antibody and free PE-Cy7 were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-STAT5A Antibody (ABIN1177247)

Product Details anti-STAT5A Antibody Target Details STAT5A Application Details Handling Images back to top
Product cited in:

Riou, Yassine-Diab, Van grevenynghe, Somogyi, Greller, Gagnon, Gimmig, Wilkinson, Shi, Cameron, Campos-Gonzalez, Balderas, Kelvin, Sekaly, Haddad: "Convergence of TCR and cytokine signaling leads to FOXO3a phosphorylation and drives the survival of CD4+ central memory T cells." in: The Journal of experimental medicine, Vol. 204, Issue 1, pp. 79-91, 2007

Bromberg, Darnell: "The role of STATs in transcriptional control and their impact on cellular function." in: Oncogene, Vol. 19, Issue 21, pp. 2468-73, 2000

Imada, Leonard: "The Jak-STAT pathway." in: Molecular immunology, Vol. 37, Issue 1-2, pp. 1-11, 2000

Gouilleux, Wakao, Mundt, Groner: "Prolactin induces phosphorylation of Tyr694 of Stat5 (MGF), a prerequisite for DNA binding and induction of transcription." in: The EMBO journal, Vol. 13, Issue 18, pp. 4361-9, 1994