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anti-Rat (Rattus) STAT5A antibody for Enzyme Immunoassay

Recommended STAT5A Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 5A (STAT5A) Antibodies
  • STAT5A
  • STAT5
  • MGF
  • AA959963
  • Stat5
  • STAT5A/MGF
  • STATA5
  • signal transducer and activator of transcription 5A
  • signal transducer and activator of transcription 5A-like
  • signal transducer and activator of transcription 5B
  • STAT5A
  • Stat5a
  • LOC100348518
  • STAT5B
Epitope
Tyr694
93
50
45
17
16
14
9
6
6
6
5
5
5
5
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
446
238
209
35
23
19
17
17
7
3
2
1
1
1
1
Host
Mouse
337
116
12
Clonality
Monoclonal
Conjugate
This STAT5A antibody is un-conjugated
12
12
10
8
6
6
5
5
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
364
220
152
84
76
69
37
31
26
18
11
8
7
6
5
4
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN400812
$ 522.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN401413 EIA IHC (p) WB Rabbit IgG Tyr694 Log in to see Polyclonal 4
1 ABIN271829 EIA IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN401412 EIA WB Rabbit IgG C-Term Log in to see Polyclonal 1
1 ABIN372786 EIA IHC (p) WB Rabbit IgG Tyr694 Log in to see Polyclonal

Top referenced anti-STAT5A antibody for Enzyme Immunoassay

Similar anti-STAT5A Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 59 Antibodies 71 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies 5 Antibodies
Flow Cytometry (FACS) 20 Antibodies 17 Antibodies
Immunochromatography (IC) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 18 Antibodies
Immunofluorescence (IF) 31 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 109 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 56 Antibodies
Immunoprecipitation (IP) 17 Antibodies
Intracellular Staining (ICS) 9 Antibodies
Western Blotting (WB) 159 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Gel Shift (GS) 1 Antibodies

General

Antigen Signal Transducer and Activator of Transcription 5A (STAT5A) Antibodies
Epitope Tyr694
(93), (50), (45), (17), (16), (14), (9), (6), (6), (6), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(446), (238), (209), (35), (23), (19), (17), (17), (7), (3), (2), (1), (1), (1), (1)
Host Mouse
(337), (116), (12)
Clonality (Clone)
Monoclonal   ( )
Conjugate This STAT5A antibody is un-conjugated
(12), (12), (10), (8), (6), (6), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(364), (220), (152), (84), (76), (69), (37), (31), (26), (18), (11), (8), (7), (6), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-STAT5A Antibody

Target Details STAT5A Application Details Handling References for anti-STAT5A Antibody (ABIN400812) Images
Specificity This phospho-specific monoclonal antibody reacts with Stat5a pTyr694 and shows minimal reactivity by ELISA against the nonphosphorylated form of the immunizing peptide.
Cross-Reactivity (Details) Species reactivity (expected):Human and Rat (100 %).
Species reactivity (tested):Mouse.
Characteristics Synonyms: STAT-5, STAT-5A, Mammary gland factor, MGF, Signal transducer and activator oftranscription 5A
Purification Protein A Chromatography from concentrated tissue culture supernate.
Immunogen Synthetic peptide corresponding to residues surrounding Tyr694 of Mouse STAT5a protein.
Clone 5F6-F1
Isotype IgG1

Target Details STAT5A

Product Details anti-STAT5A Antibody Application Details Handling References for anti-STAT5A Antibody (ABIN400812) Images back to top
Antigen
Alternative Name STAT5 / STAT5A (STAT5A Antibody Abstract)
Background Signal transducer and activator of transcription 5 (Stat5) belongs to a family of cytoplasmic transcription factors that can be activated (phosphorylated) by a cell surface receptor. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Stat5 has two isoforms, Stat5alpha and Stat5beta. Aberrant Stat5 activation has been implicated in the pathogenesis of chronic myelogenous leukemia, prostate and breast cancer and tumor metastasis. Stat5 is localized in the cytoplasm and upon phosphorylation at Y694 is translocated to the nucleus.Synonyms: MGF, Mammary gland factor, STAT-5, STAT-5A, Signal transducer and activator of transcription 5A
Gene ID 20850
UniProt P42230
Pathways JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity

Application Details

Product Details anti-STAT5A Antibody Target Details STAT5A Handling References for anti-STAT5A Antibody (ABIN400812) Images back to top
Application Notes ELISA: 1/20,000. Western blot: 1/500-1/2,000 (Expect a band approximately 91 kDa in size corresponding tophosphorylated Stat5α protein in the appropriate cell lysate or extract). Immunohistochemistry (20-40 μg/mL). Immunofluorescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-STAT5A Antibody Target Details STAT5A Application Details References for anti-STAT5A Antibody (ABIN400812) Images back to top
Concentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store vial at -20 °C or below prior to opening. For low volume of reagent (25 μl), to minimizeloss of volume dilute 1/10 by adding 225 μl of the buffer stated abovedirectly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at thebottom of the vial. Use this intermediate dilution when calculating final dilutions asrecommended below. Store the vial at -20 °C or below after dilution. Avoid cycles of freezing and thawing.
Shelf life: One year from despatch (3 months from date of opening).
Expiry Date 12 months

References for anti-STAT5A Antibody (ABIN400812)

Product Details anti-STAT5A Antibody Target Details STAT5A Application Details Handling Images back to top
Background publications

Engblom, Kornfeld, Schwake, Tronche, Reimann, Beug, Hennighausen, Moriggl, Schütz: "Direct glucocorticoid receptor-Stat5 interaction in hepatocytes controls body size and maturation-related gene expression." in: Genes & development, Vol. 21, Issue 10, pp. 1157-62, 2007

Baugh, Floyd, Stephens: "The modulation of STAT5A/GR complexes during fat cell differentiation and in mature adipocytes." in: Obesity (Silver Spring, Md.), Vol. 15, Issue 3, pp. 583-90, 2007

Laurence, Tato, Davidson, Kanno, Chen, Yao, Blank, Meylan, Siegel, Hennighausen, Shevach, Oshea: "Interleukin-2 signaling via STAT5 constrains T helper 17 cell generation." in: Immunity, Vol. 26, Issue 3, pp. 371-81, 2007

Burchill, Yang, Vogtenhuber, Blazar, Farrar: "IL-2 receptor beta-dependent STAT5 activation is required for the development of Foxp3+ regulatory T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 1, pp. 280-90, 2006

Images

Product Details anti-STAT5A Antibody Target Details STAT5A Application Details Handling References for anti-STAT5A Antibody (ABIN400812) back to top
Supplier Images
Western Blotting (WB) image for anti-STAT5A antibody (Signal Transducer and Activator of Transcription 5A) (Tyr694) (ABIN400812) anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (Tyr694) antibody
Immunohistochemistry (IHC) image for anti-STAT5A antibody (Signal Transducer and Activator of Transcription 5A) (Tyr694) (ABIN400812) anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (Tyr694) antibody (Image 2)
Immunofluorescence (IF) image for anti-STAT5A antibody (Signal Transducer and Activator of Transcription 5A) (Tyr694) (ABIN400812) anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (Tyr694) antibody (Image 3)