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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||59 Antibodies||71 Antibodies|
|Enzyme Immunoassay (EIA)||5 Antibodies||5 Antibodies|
|Flow Cytometry (FACS)||20 Antibodies||17 Antibodies|
|Immunochromatography (IC)||1 Antibodies||1 Antibodies|
|Immunocytochemistry (ICC)||18 Antibodies|
|Immunofluorescence (IF)||31 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||26 Antibodies|
|Immunohistochemistry (IHC)||109 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||56 Antibodies|
|Immunoprecipitation (IP)||17 Antibodies|
|Intracellular Staining (ICS)||9 Antibodies|
|Western Blotting (WB)||159 Antibodies|
|Fluorescence Microscopy (FM)||1 Antibodies|
|Gel Shift (GS)||1 Antibodies|
|Antigen||Signal Transducer and Activator of Transcription 5A (STAT5A) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This STAT5A antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|4 references available|
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Product Details anti-STAT5A AntibodyTarget Details STAT5A Application Details Handling References for anti-STAT5A Antibody (ABIN400812) Images
|Specificity||This phospho-specific monoclonal antibody reacts with Stat5a pTyr694 and shows minimal reactivity by ELISA against the nonphosphorylated form of the immunizing peptide.|
Species reactivity (expected):Human and Rat (100 %).
Species reactivity (tested):Mouse.
|Characteristics||Synonyms: STAT-5, STAT-5A, Mammary gland factor, MGF, Signal transducer and activator oftranscription 5A|
|Purification||Protein A Chromatography from concentrated tissue culture supernate.|
|Immunogen||Synthetic peptide corresponding to residues surrounding Tyr694 of Mouse STAT5a protein.|
Target Details STAT5AProduct Details anti-STAT5A Antibody Application Details Handling References for anti-STAT5A Antibody (ABIN400812) Images back to top
|Alternative Name||STAT5 / STAT5A (STAT5A Antibody Abstract)|
|Background||Signal transducer and activator of transcription 5 (Stat5) belongs to a family of cytoplasmic transcription factors that can be activated (phosphorylated) by a cell surface receptor. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Stat5 has two isoforms, Stat5alpha and Stat5beta. Aberrant Stat5 activation has been implicated in the pathogenesis of chronic myelogenous leukemia, prostate and breast cancer and tumor metastasis. Stat5 is localized in the cytoplasm and upon phosphorylation at Y694 is translocated to the nucleus.Synonyms: MGF, Mammary gland factor, STAT-5, STAT-5A, Signal transducer and activator of transcription 5A|
|Pathways||JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity|
Application DetailsProduct Details anti-STAT5A Antibody Target Details STAT5A Handling References for anti-STAT5A Antibody (ABIN400812) Images back to top
ELISA: 1/20,000. Western blot: 1/500-1/2,000 (Expect a band approximately 91 kDa in size corresponding tophosphorylated Stat5α protein in the appropriate cell lysate or extract). Immunohistochemistry (20-40 μg/mL). Immunofluorescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-STAT5A Antibody Target Details STAT5A Application Details References for anti-STAT5A Antibody (ABIN400812) Images back to top
|Concentration||1.0 mg/mL (by UV absorbance at 280 nm)|
|Buffer||0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
Store vial at -20 °C or below prior to opening. For low volume of reagent (25 μl), to minimizeloss of volume dilute 1/10 by adding 225 μl of the buffer stated abovedirectly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at thebottom of the vial. Use this intermediate dilution when calculating final dilutions asrecommended below. Store the vial at -20 °C or below after dilution. Avoid cycles of freezing and thawing.
Shelf life: One year from despatch (3 months from date of opening).
|Expiry Date||12 months|
References for anti-STAT5A Antibody (ABIN400812)Product Details anti-STAT5A Antibody Target Details STAT5A Application Details Handling Images back to top
Engblom, Kornfeld, Schwake, Tronche, Reimann, Beug, Hennighausen, Moriggl, Schütz: "Direct glucocorticoid receptor-Stat5 interaction in hepatocytes controls body size and maturation-related gene expression." in: Genes & development, Vol. 21, Issue 10, pp. 1157-62, 2007
Baugh, Floyd, Stephens: "The modulation of STAT5A/GR complexes during fat cell differentiation and in mature adipocytes." in: Obesity (Silver Spring, Md.), Vol. 15, Issue 3, pp. 583-90, 2007
Laurence, Tato, Davidson, Kanno, Chen, Yao, Blank, Meylan, Siegel, Hennighausen, Shevach, Oshea: "Interleukin-2 signaling via STAT5 constrains T helper 17 cell generation." in: Immunity, Vol. 26, Issue 3, pp. 371-81, 2007
Burchill, Yang, Vogtenhuber, Blazar, Farrar: "IL-2 receptor beta-dependent STAT5 activation is required for the development of Foxp3+ regulatory T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 1, pp. 280-90, 2006
ImagesProduct Details anti-STAT5A Antibody Target Details STAT5A Application Details Handling References for anti-STAT5A Antibody (ABIN400812) back to top