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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||52 Antibodies||65 Antibodies|
|Enzyme Immunoassay (EIA)||5 Antibodies||5 Antibodies|
|Flow Cytometry (FACS)||5 Antibodies||9 Antibodies|
|Immunochromatography (IC)||1 Antibodies||1 Antibodies|
|Immunocytochemistry (ICC)||17 Antibodies||18 Antibodies|
|Immunofluorescence (IF)||28 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies|
|Immunohistochemistry (IHC)||105 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||45 Antibodies|
|Immunoprecipitation (IP)||13 Antibodies|
|Intracellular Staining (ICS)||9 Antibodies|
|Western Blotting (WB)||136 Antibodies|
|Fluorescence Microscopy (FM)||1 Antibodies|
|Gel Shift (GS)||1 Antibodies|
|Antigen||Signal Transducer and Activator of Transcription 5A (STAT5A) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This STAT5A antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-STAT5A AntibodyTarget Details STAT5A Application Details Handling References for anti-STAT5A Antibody (ABIN401413) Images
|Specificity||This antibody is specific for Stat5alpha protein phosphorylated at Y694.|
Species reactivity (expected):Rat, Human.
Species reactivity (tested):Mouse.
|Immunogen||synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5alpha protein|
Target Details STAT5AProduct Details anti-STAT5A Antibody Application Details Handling References for anti-STAT5A Antibody (ABIN401413) Images back to top
|Alternative Name||STAT5 / STAT5A (STAT5A Antibody Abstract)|
|Background||Signal trans-ducer and activator of transcription 5 (Stat5) belongs to a family of cytoplasmic trans-cription factors that can be activated (phosphorylated) by a cell surface receptor. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Stat5 has two isoforms, Stat5alpha and Stat5beta. Aberrant Stat5 activation has been implicated in the pathogenesis of chronic myelogenous leukemia, prostate and breast cancer and tumor metastasis. Stat5 is localized in the cytoplasm and upon phosphorylation at Y694 is translocated to the nucleus.Synonyms: MGF, Mammary gland factor, STAT-5, STAT-5A, Signal transducer and activator of transcription 5A|
|Pathways||JAK-STAT Signaling, RTK Signaling|
Application DetailsProduct Details anti-STAT5A Antibody Target Details STAT5A Handling References for anti-STAT5A Antibody (ABIN401413) Images back to top
ELISA: 1/25,000. Western Blot: 1/500 - 1/2,000. Immunohistochemistry: 20 - 40 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-STAT5A Antibody Target Details STAT5A Application Details References for anti-STAT5A Antibody (ABIN401413) Images back to top
|Concentration||1.1 mg/mL (by UV absorbance at 280 nm)|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
References for anti-STAT5A Antibody (ABIN401413)Product Details anti-STAT5A Antibody Target Details STAT5A Application Details Handling Images back to top
Baugh, Floyd, Stephens: "The modulation of STAT5A/GR complexes during fat cell differentiation and in mature adipocytes." in: Obesity (Silver Spring, Md.), Vol. 15, Issue 3, pp. 583-90, 2007
Laurence, Tato, Davidson, Kanno, Chen, Yao, Blank, Meylan, Siegel, Hennighausen, Shevach, Oshea: "Interleukin-2 signaling via STAT5 constrains T helper 17 cell generation." in: Immunity, Vol. 26, Issue 3, pp. 371-81, 2007
Engblom, Kornfeld, Schwake, Tronche, Reimann, Beug, Hennighausen, Moriggl, Schütz: "Direct glucocorticoid receptor-Stat5 interaction in hepatocytes controls body size and maturation-related gene expression." in: Genes & development, Vol. 21, Issue 10, pp. 1157-62, 2007
Burchill, Yang, Vogtenhuber, Blazar, Farrar: "IL-2 receptor beta-dependent STAT5 activation is required for the development of Foxp3+ regulatory T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 1, pp. 280-90, 2006
ImagesProduct Details anti-STAT5A Antibody Target Details STAT5A Application Details Handling References for anti-STAT5A Antibody (ABIN401413) back to top