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|Antigen||Signal Transducer and Activator of Transcription 5B (STAT5B) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This STAT5B antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-STAT5B AntibodyTarget Details STAT5B Application Details Handling Images
|Specificity||This antibody detects endogenous levels of Stat5b protein.|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||Affinity chromatography using epitope-specific immunogen|
|Purity||> 95 % (by SDS-PAGE)|
Target Details STAT5BProduct Details anti-STAT5B Antibody Application Details Handling Images back to top
|Alternative Name||STAT5B (STAT5B Antibody Abstract)|
|Background||Stat5a and Stat5b (signal transducer and activator of transcription 5a and 5b, 96 % homology) undergo receptor tyrosine kinase or G protein-coupled receptor-dependent phosphorylation in response to cytokines or growth factors, and then form homo- or heterodimers that translocate to the nucleus where they initiate transcription. Activation of Stat5a via IL-2, IL-3, IL-7, GM-CSF, erythropoietin, thrombopoietin and growth hormones influences proliferation, differentiation and apoptosis in lymphohematopoietic cells. Phosphorylation of Stat5a at Ser 127/Ser 128 and Ser 779 are contigent on ErbB4-mediated activation of Stat5a. Activation of Stat5b via IL-2, IL-4, CSF1 and growth hormones influences TCR signaling, apoptosis, adult mammary gland development and sexual dimorphism of liver gene expression. Stat5b is the major liver-expressed Stat5 form that has been shown to fuse with retinoic acid receptor-α gene in acute promyelocytic leukemias (APLL). Stat5a/b null mice have severely impaired lymphoid development and differentiation.Synonyms: STAT-5B, Signal transducer and activator of transcription 5B|
|Molecular Weight||approx. 90 kDa|
|Pathways||JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation|
Application DetailsProduct Details anti-STAT5B Antibody Target Details STAT5B Handling Images back to top
Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-STAT5B Antibody Target Details STAT5B Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-STAT5B Antibody Target Details STAT5B Application Details Handling back to top