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Mouse (Murine) Thrombopoietin ELISA Kit

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Antigen
Thrombopoietin (THPO) ELISA Kits
  • LOC100231762
  • Mgdf
  • MGDF
  • MKCSF
  • Ml
  • ML
  • Mpllg
  • MPLLG
  • THCYT1
  • Tpo
  • TPO
  • tpo
  • Tpo1
  • Tpo2
  • Tpo3
  • Tpo4
Epitope
AA 22-356
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
17
15
9
6
5
4
3
3
2
2
1
1
Methode Type
Sandwich ELISA
Detection Range
31.2-2000 pg/mL
Minimum Detection Limit
31.2 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN411356
$ 340.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.3084228 ABIN415539 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.3084228 ABIN1117429 Sandwich ELISA Serum, Plasma, Biological Fluids 7.813-500 pg/mL 7.81 pg/mL Log in to see
0.3084228 ABIN2708607 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.001213088 ABIN367719 Sandwich ELISA Serum, Plasma, Tissue Homogenate 6.25-400 pg/mL 6.25 pg/mL Log in to see
0.001213088 ABIN628209 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 100-2500 pg/mL 100 pg/mL Log in to see
0.001213088 ABIN456568 Sandwich ELISA Serum, Plasma 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.001213088 ABIN625183 Sandwich ELISA Plasma, Serum, Cell Culture Supernatant, Cell and Tissue Culture Lysate, Body Fluids 30-20000 pg/mL 30 pg/mL Log in to see
0.001213088 ABIN510894 Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate Log in to see
0.001213088 ABIN1112691 Sandwich ELISA Serum, Body Fluids, Tissue Lysate, Cell Culture Supernatant 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.001213088 ABIN2116353 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.001213088 ABIN2685647 Sandwich ELISA Cell Lysate, Plasma, Serum Log in to see
0.001213088 ABIN2539525 Sandwich ELISA Serum, Plasma, Biological Fluids 7.81-500 pg/mL 7.81 pg/mL Log in to see
0.001213088 ABIN2107846 Sandwich ELISA Log in to see
0.001213088 ABIN2951790 7.813-500 pg/mL 7.813 pg/mL Log in to see
0.001213088 ABIN1720541 Log in to see

Top referenced Thrombopoietin ELISA Kit for ELISA

Similar Thrombopoietin ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 16 ELISA Kits

General

Antigen Thrombopoietin (THPO) ELISA Kits
Epitope AA 22-356
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(17), (15), (9), (6), (5), (4), (3), (3), (2), (2), (1), (1)
Methode Type Sandwich ELISA
Detection Range 31.2-2000 pg/mL
Minimum Detection Limit 31.2 pg/mL
Application ELISA
Pubmed 2 references available
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Product Details Thrombopoietin ELISA Kit

Target details Application Details Handling References for Thrombopoietin Kit (ABIN411356) Images
Purpose For quantitative detection of mouse TPO in cell culture supernates, serum and plasma( heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant mouse TPO
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <5pg/mL
Components
  • 96-well plate precoated with anti- mouse TPO antibod - 1
  • Lyophilized recombinant mouse TPO standar - 10ng/tubex2
  • Biotinylated anti- mouse TPO antibod - 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: S22-T356

Target details

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Antigen
Alternative Name Thrombopoietin (TPO) (THPO ELISA Kit Abstract)
Background Thrombopoietin(TPO) is also called megakaryocyte growth and development factor(MGDF). TPO, a lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from committed progenitor cells, is believed to be the major physiological regulator of circulating platelet levels. Human, dog, and mouse cDNAs for MGDF are highly conserved and encode open reading frames for proteins of 353, 352, and 356 amino acids, respectively, including predicted signal peptides. MGDF is a novel cytokine that regulates megakaryocyte development and is a ligand for the MPL receptor. The human MGDF gene has been mapped to chromosome 3q26.3. The standard product used in this kit is gene recombinant mouse TPO, consisting of 335 amino acids with the molecular mass of 35KDa. As a result of glycosylation, the molecular mass is 75KDa.
Gene ID 21832
UniProt P40226
Pathways JAK-STAT Signaling

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol mouse TPO ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TPO has been precoated onto 96-well plates. Standards(NSO, S22-T356) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TPO is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TPO amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse TPO standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse ell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TPO standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 296, Standard deviation: 11.84, CV(%): 4
  • Sample 2: n=16, Mean(pg/ml): 611, Standard deviation: 33, CV(%): 5.4
  • Sample 3: n=16, Mean(pg/ml): 1265, Standard deviation: 82.23, CV(%): 6.5,
  • Sample 1: n=24, Mean(pg/ml): 321, Standard deviation: 16.7, CV(%): 5.2
  • Sample 2: n=24, Mean(pg/ml): 746, Standard deviation: 47, CV(%): 6.3
  • Sample 3: n=24, Mean(pg/ml): 1464, Standard deviation: 105.4, CV(%): 7.2
Restrictions For Research Use only

Handling

Product Details Thrombopoietin ELISA Kit Target details Application Details References for Thrombopoietin Kit (ABIN411356) Images back to top
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for Thrombopoietin Kit (ABIN411356)

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Background publications

Chang, McNinch, Basu, Shutter, Hsu, Perkins, Mar, Suggs, Welcher, Li: "Cloning and characterization of the human megakaryocyte growth and development factor (MGDF) gene." in: The Journal of biological chemistry, Vol. 270, Issue 2, pp. 511-4, 1995

Bartley, Bogenberger, Hunt, Li, Lu, Martin, Chang, Samal, Nichol, Swift: "Identification and cloning of a megakaryocyte growth and development factor that is a ligand for the cytokine receptor Mpl." in: Cell, Vol. 77, Issue 7, pp. 1117-24, 1994

Images

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Supplier Images
ELISA image for Thrombopoietin ELISA Kit (THPO) (ABIN411356) Mouse TPO PicoKine ELISA Kit standard curve