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Mouse (Murine) TYK2 ELISA Kit

Recommended TYK2 Product (supplied by: Log in to see )

Antigen
Tyrosine Kinase 2 (TYK2) ELISA Kits
  • JTK1
  • tyrosine kinase 2
  • tyrosine kinase 2-like
  • TYK2
  • Tyk2
  • LOC100344622
Epitope
pTyr1054, total
Reactivity
Human, Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
12
2
1
1
1
1
Method Type
Sandwich ELISA
Application
ELISA
Options
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Catalog No. ABIN4886339
$ 628.10
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
1 ABIN4886336 Sandwich ELISA Cell and Tissue Culture Lysate Log in to see
1 ABIN1145271 Log in to see

Similar TYK2 ELISA Kits

Application / Reactivity Human Mouse (Murine)
ELISA 13 ELISA Kits 3 ELISA Kits

General

Antigen Tyrosine Kinase 2 (TYK2) ELISA Kits
Epitope pTyr1054, total
Reactivity Human, Mouse (Murine)
Kits with alternative reactivity to:
(12), (2), (1), (1), (1), (1)
Method Type Sandwich ELISA
Application ELISA
Supplier Log in to see

Product Details TYK2 ELISA Kit

Target details Application Details Handling Images
Purpose Human and Mouse Phospho-TYK2 (Tyr1054) and Total TYK2 ELISA Kit. This assay semi-quantitatively measures phophorylated TYK2 (Tyr1054) and Total TYK2 in lysate samples.
Sample Type Cell and Tissue Culture Lysate
Detection Method Colorimetric
Analytical Method Semi-Quantitative
Specificity This ELISA kit recognizes Human and Mouse TYK2 phosphorylated at site Tyrosine-1054 as well as total TYK2.
Characteristics
  • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
  • Screen numerous different cell lysates without performing a Western Blot analysis
  • Minimal hands-on time, convenient, and non-radioactive material
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Anti-Phospho Antibody
  • Anti-Pan Antibody
  • HRP-Conjugated Secondary Antibody
  • Streptavidin-Conjugated HRP
  • Assay Diluent
  • TMB One-Step Substrate
  • Stop Solution
  • Lysis Buffer
  • Positive Control Sample
Material not included
  • Distilled or deionized water
  • 100 mL and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 μL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm

Target details

Product Details TYK2 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name TYK2 (TYK2 ELISA Kit Abstract)
Gene ID 7297
UniProt P29597, Q9R117
Research Area Signaling, Tyrosine Kinases
Pathways JAK-STAT Signaling, Hepatitis C

Application Details

Product Details TYK2 ELISA Kit Target details Handling Images back to top
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 μL of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only

Handling

Product Details TYK2 ELISA Kit Target details Application Details Images back to top
Storage -20 °C
Storage Comment Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Expiry Date 6 months