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RFP-Trap® M (coupled to magnetic beads)
Immunoprecipitation (IP), Mass Spectrometry (MS), Enzyme Activity Assay (EAA)
|5 references available|
|Price||254.10 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 4 to 5 Business Days|
|Description||Red fluorescent proteins (RFP) and variants thereof are widely used to study protein localization and dynamics. For biochemical analyses including mass spectroscopy and enzyme activity measurements these RFP fusion proteins and their interacting factors can be isolated fast and efficiently (one step) via immunoprecipitation using the RFP-Trap®. Since the interaction is mediated by a small RFP binding protein coupled to agarose beads the RFPTrap®_M enables purification of any protein of interest fused to RFP (monomeric derivates of DsRed, including mRFP1, mCherry, mPlum, mOrange).|
|Characteristics||particle size 0.5 - 1 µm|
|Specificity||RFP-Trap® efficiently pulls down most common monomeric red fluorescent proteins derived from DsRed, e.g. mRFP1, mCherry, mPlum and RFP fusion proteins. No cross-reaction to GFP derivates can be detected.|
|Principle||for biochemical analysis of RFP fusion proteins and their interacting partners.|
|Preservative||0.05% Sodium azide|
|Storage||Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 6 months. Do not freeze.|
|Restrictions||For Research Use only|
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