IgG ELISA Kit
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- Target See all IgG ELISA Kits
- IgG
- Binding Specificity
- Heavy & Light Chain
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Reactivity
- Cow
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 10-2000 ng/mL
- Minimum Detection Limit
- 10 ng/mL
- Application
- ELISA
- Purpose
- Rapid quantification of bovine IgG in batches of purified antibodies, selection of low IgG sera
- Brand
- FastELISA®
- Sample Type
- Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- Specificity
- The method enables the detection of bovine IgG.
- Characteristics
- The bovine immunoglobulin quantification kit provides a rapid and easy method (one step ELISA) for the quantitative determination of bovine IgG in cell culture supernatants and serums.
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- Discover our best selling IgG ELISA Kit
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- Discover our top product IgG ELISA Kit
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IgG ELISA Kit
Reactivity: Rabbit Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA KitReactivity: Mouse Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA KitReactivity: Human Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA KitReactivity: Chicken Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA KitReactivity: Rat Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA KitReactivity: Pig Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA KitReactivity: Cow Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
Rabbit TrueBlot® Set (with IP beads)WB, IP Reactivity: Rabbit
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- Comment
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One Step ELISA 45 min total time
IgG contaminations of about 20 ppm - Assay Time
- < 1 h
- Plate
- Pre-coated
- Protocol
- A polyclonal antibody specific to bovine IgG is pre-coated onto microwells. Samples and standards are pipetted into microwells and bovine IgG present in the sample are bound by the capture antibody. Then, an HRP (horseradish peroxidase) conjugated anti-bovine IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of bovine IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
- Reagent Preparation
-
dilution
- Restrictions
- For Research Use only
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- Storage
- 4 °C
- Expiry Date
- 12 months
-
- Target See all IgG ELISA Kits
- IgG
- Alternative Name
- Immunoglobulin G (IgG Products)
- Target Type
- Antibody
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